Browsing by Author "Frawley, Andrew T."
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- ItemSuper-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad(Cambridge : RSC, 2020) Frawley, Andrew T.; Wycisk, Virginia; Xiong, Yaoyao; Galiani, Silvia; Sezgin, Erdinc; Urbančič, Iztok; Vargas Jentzsch, Andreas; Leslie, Kathryn G.; Eggeling, Christian; Anderson, Harry L.Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm). This journal is © The Royal Society of Chemistry.