Browsing by Author "Linxweiler, Johannes"

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    Proteomic insights into non-small cell lung cancer: New ideas for cancer diagnosis and therapy from a functional viewpoint
    (Amsterdam : Elsevier, 2014) Linxweiler, Johannes; Kollipara, Laxmikanth; Zahedi, René P.; Lampel, Pavel; Zimmermann, Richard; Greiner, Markus
    We recently characterized SEC62 as an oncogene in non-small-cell lung cancer (NSCLC). Here we aimed to gain further insight into the molecular mechanisms of the cancer-related functions of this oncogene. We performed 2D-DIGE proteome analysis of tumor material from patients with NSCLC and of HEK293 cells stably overexpressing plasmid-encoded SEC62, combined with investigation of the Sec62 interactome. Furthermore, we analyzed the proteomic effects of siRNA-mediated depletion of the Sec62-interacting protein Sec63. We identified a comprehensive list of differentially regulated proteins, providing new insights into the molecular mechanisms of the cancer-related functions of Sec62 in cell migration, drug resistance, and Ca2+-homeostasis.