Isolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopy

dc.bibliographicCitation.firstPage5193eng
dc.bibliographicCitation.issue20eng
dc.bibliographicCitation.journalTitleAnalytical and bioanalytical chemistryeng
dc.bibliographicCitation.lastPage5200eng
dc.bibliographicCitation.volume413eng
dc.contributor.authorWichmann, Christina
dc.contributor.authorRösch, Petra
dc.contributor.authorPopp, Jürgen
dc.date.accessioned2022-01-25T14:13:02Z
dc.date.available2022-01-25T14:13:02Z
dc.date.issued2021
dc.description.abstractRaman spectroscopy is an analytical method to identify medical samples of bacteria. Because Raman spectroscopy detects the biochemical properties of a cell, there are many factors that can influence and modify the Raman spectra of bacteria. One possible influence is a proper method for isolation of the bacteria. Medical samples in particular never occur in purified form, so a Raman-compatible isolation method is needed which does not affect the bacteria and thus the resulting spectra. In this study, we present a Raman-compatible method for isolation of bacteria from bronchoalveolar lavage (BAL) fluid using density gradient centrifugation. In addition to measuring the bacteria from a patient sample, the yield and the spectral influence of the isolation on the bacteria were investigated. Bacteria isolated from BAL fluid show additional peaks in comparison to pure culture bacteria, which can be attributed to components in the BAL sample. The isolation gradient itself has no effect on the spectra, and with a yield of 63% and 78%, the method is suitable for isolation of low concentrations of bacteria from a complex matrix. Graphical abstract.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/7931
dc.identifier.urihttps://doi.org/10.34657/6972
dc.language.isoengeng
dc.publisherBerlin [u.a.] : Springereng
dc.relation.doihttps://doi.org/10.1007/s00216-021-03488-0
dc.relation.essn1618-2650
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc540eng
dc.subject.otherBacteriaeng
dc.subject.otherIsolationeng
dc.subject.otherRaman spectroscopyeng
dc.titleIsolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopyeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
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