Effect of Cereal α-Amylase/Trypsin Inhibitors on Developmental Characteristics and Abundance of Digestive Enzymes of Mealworm Larvae (Tenebrio molitor L.)

dc.bibliographicCitation.firstPage454eng
dc.bibliographicCitation.issue5eng
dc.bibliographicCitation.journalTitleInsects : open access journaleng
dc.bibliographicCitation.volume12eng
dc.contributor.authorSagu, Sorel Tchewonpi
dc.contributor.authorLandgräber, Eva
dc.contributor.authorHenkel, Ina M
dc.contributor.authorHuschek, Gerd
dc.contributor.authorHomann, Thomas
dc.contributor.authorBußler, Sara
dc.contributor.authorSchlüter, Oliver K.
dc.contributor.authorRawel, Harshadrai
dc.date.accessioned2022-03-03T13:28:45Z
dc.date.available2022-03-03T13:28:45Z
dc.date.issued2021
dc.description.abstractThe objective of this work was to investigate the potential effect of cereal α-amylase/trypsin inhibitors (ATIs) on growth parameters and selective digestive enzymes of Tenebrio molitor L. larvae. The approach consisted of feeding the larvae with wheat, sorghum and rice meals containing different levels and composition of α-amylase/trypsin inhibitors. The developmental and biochemical characteristics of the larvae were assessed over feeding periods of 5 h, 5 days and 10 days, and the relative abundance of α-amylase and selected proteases in larvae were determined using liquid chromatography tandem mass spectrometry. Overall, weight gains ranged from 21% to 42% after five days of feeding. The larval death rate significantly increased in all groups after 10 days of feeding (p < 0.05), whereas the pupation rate was about 25% among larvae fed with rice (Oryza sativa L.) and Siyazan/Esperya wheat meals, and only 8% and 14% among those fed with Damougari and S35 sorghum meals. As determined using the Lowry method, the protein contents of the sodium phosphate extracts ranged from 7.80 ± 0.09 to 9.42 ± 0.19 mg/mL and those of the ammonium bicarbonate/urea reached 19.78 ± 0.16 to 37.47 ± 1.38 mg/mL. The total protein contents of the larvae according to the Kjeldahl method ranged from 44.0 and 49.9 g/100 g. The relative abundance of α-amylase, CLIP domain-containing serine protease, modular serine protease zymogen and C1 family cathepsin significantly decreased in the larvae, whereas dipeptidylpeptidase I and chymotrypsin increased within the first hours after feeding (p < 0.05). Trypsin content was found to be constant independently of time or feed material. Finally, based on the results we obtained, it was difficult to substantively draw conclusions on the likely effects of meal ATI composition on larval developmental characteristics, but their effects on the digestive enzyme expression remain relevant.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/8136
dc.identifier.urihttps://doi.org/10.34657/7176
dc.language.isoengeng
dc.publisherBasel : MDPIeng
dc.relation.doihttps://doi.org/10.3390/insects12050454
dc.relation.essn2075-4450
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc570eng
dc.subject.otherCereal mealseng
dc.subject.otherDigestive enzymes quantificationeng
dc.subject.otherFeedingeng
dc.subject.otherGrowth behavioreng
dc.subject.otherLC-MS/MSeng
dc.subject.otherTenebrio molitor larvaeeng
dc.subject.otherα-amylase/trypsin inhibitorseng
dc.titleEffect of Cereal α-Amylase/Trypsin Inhibitors on Developmental Characteristics and Abundance of Digestive Enzymes of Mealworm Larvae (Tenebrio molitor L.)eng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorATBeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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