T cell stiffness is enhanced upon formation of immunological synapse
dc.bibliographicCitation.firstPage | e66643 | |
dc.bibliographicCitation.volume | 10 | |
dc.contributor.author | Jung, Philipp | |
dc.contributor.author | Zhou, Xiangda | |
dc.contributor.author | Iden, Sandra | |
dc.contributor.author | Bischoff, Markus | |
dc.contributor.author | Qu, Bin | |
dc.date.accessioned | 2022-03-10T12:41:27Z | |
dc.date.available | 2022-03-10T12:41:27Z | |
dc.date.issued | 2021 | |
dc.description.abstract | T cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~60 nm. Using primary human CD4+ T cells, we show that when T cells form IS with stimulating antibody-coated surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is enhanced both at the lamellipodia and on the cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell-body-stiffening, suggesting different regulatory mechanisms of IS-induced stiffening at the lamellipodia and the cell body. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/8217 | |
dc.identifier.uri | https://doi.org/10.34657/7255 | |
dc.language.iso | eng | eng |
dc.publisher | Cambridge : eLife Sciences Publications | |
dc.relation.doi | https://doi.org/10.7554/eLife.66643 | |
dc.relation.essn | 2050-084X | |
dc.relation.ispartofseries | eLife 10 (2021) | |
dc.rights.license | CC BY 4.0 Unported | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | T cells | eng |
dc.subject | stiffness | eng |
dc.subject | immunological synapse | eng |
dc.subject | lamellipodia | eng |
dc.subject | calcium | eng |
dc.subject | primary CD4+ T cells | eng |
dc.subject | Jurkat T-cells | eng |
dc.subject.ddc | 500 | |
dc.subject.ddc | 600 | |
dc.title | T cell stiffness is enhanced upon formation of immunological synapse | eng |
dc.type | article | eng] |
dc.type | Text | eng] |
dcterms.bibliographicCitation.journalTitle | eLife | |
tib.accessRights | openAccess | eng |
wgl.contributor | INM | ger |
wgl.subject | Medizin, Gesundheit | ger |
wgl.subject | Physik | ger |
wgl.type | Zeitschriftenartikel | ger |
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