Model-based characterisation of growth performance and l-lactic acid production with high optical purity by thermophilic Bacillus coagulans in a lignin-supplemented mixed substrate medium

dc.bibliographicCitation.firstPage180
dc.bibliographicCitation.journalTitleNew biotechnologyeng
dc.bibliographicCitation.lastPage193
dc.bibliographicCitation.volume37
dc.contributor.authorGlaser, Robert
dc.contributor.authorVenus, Joachim
dc.date.accessioned2022-12-19T11:49:58Z
dc.date.available2022-12-19T11:49:58Z
dc.date.issued2017
dc.description.abstractThree Bacillus coagulans strains were characterised in terms of their ability to grow in lignin-containing fermentation media and to consume the lignocellulose-related sugars glucose, xylose, and arabinose. An optical-density high-throughput screening was used for precharacterisation by means of different mathematical models for comparison (Logistic, Gompertz, Baranyi, Richards & Stannard, and Schnute). The growth response was characterised by the maximum growth rate and lag time. For a comparison of the screening and fermentation results, an unstructured mathematical model was proposed to characterise the lactate production, bacterial growth and substrate consumption. The growth model was then applied to fermentation procedures using wheat straw hydrolysates. The results indicated that the unstructured growth model can be used to evaluate lactate producing fermentation. Under the experimental fermentation conditions, one strain showed the ability to tolerate a high lignin concentration (2.5 g/L) but lacked the capacity for sufficient pentose uptake. The lactate yield of the strains that were able to consume all sugar fractions of glucose, xylose and arabinose was ∼83.4%. A photometric measurement at 280 nm revealed a dynamic change in alkali-lignin concentrations during lactate producing fermentation. A test of decolourisation of vanillin, ferulic acid, and alkali-lignin samples also showed the decolourisation performance of the B. coagulans strains under study. © 2017 The Author(s)eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/10666
dc.identifier.urihttp://dx.doi.org/10.34657/9702
dc.language.isoeng
dc.publisherNew York, NY [u.a.] : Elsevier
dc.relation.doihttps://doi.org/10.1016/j.nbt.2016.12.006
dc.relation.essn1876-4347
dc.rights.licenseCC BY-NC-ND 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc570
dc.subject.ddc540
dc.subject.otherArabinoseeng
dc.subject.otherCofermentationeng
dc.subject.otherGlucoseeng
dc.subject.otherL-Lactateeng
dc.subject.otherLignineng
dc.subject.otherUnstructured growth modeleng
dc.subject.otherXyloseeng
dc.titleModel-based characterisation of growth performance and l-lactic acid production with high optical purity by thermophilic Bacillus coagulans in a lignin-supplemented mixed substrate mediumeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorATB
wgl.subjectBiowissenschaften/Biologieger
wgl.subjectChemieger
wgl.typeZeitschriftenartikelger
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