simpleISM—A straight forward guide to upgrade from confocal to ISM

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dc.bibliographicCitation.firstPagee0279378
dc.bibliographicCitation.issue12
dc.bibliographicCitation.journalTitlePLOS ONEeng
dc.bibliographicCitation.volume17
dc.contributor.authorGoswami, Monalisa
dc.contributor.authorLachmann, René
dc.contributor.authorKretschmer, Robert
dc.contributor.authorHeintzmann, Rainer
dc.date.accessioned2023-02-22T06:09:19Z
dc.date.available2023-02-22T06:09:19Z
dc.date.issued2022
dc.description.abstractResolution in a confocal laser scanning microscopes (CLSM) can be improved if the pinhole is closed. But closing the pinhole will deteriorate the signal to noise ratio (SNR). A simple technique to improve the SNR while keeping the resolution same by upgrading the system to an image scanning microscope. In this paper, we explain in detail, based on an Olympus Fluoview 300 system, how a scanning microscope can be upgraded into an image scanning microscope (ISM) using a simple camera-based detector and an Arduino Due providing a galvo driving and camera synchronization signals. We could confirm a resolution improvement as well as superconcentration and made the interesting observation of a reduced influence of laser fluctuations.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/11485
dc.identifier.urihttp://dx.doi.org/10.34657/10518
dc.language.isoeng
dc.publisherSan Francisco, California, US : PLOS
dc.relation.doihttps://doi.org/10.1371/journal.pone.0279378
dc.relation.essn1932-6203
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subject.ddc500
dc.subject.ddc610
dc.subject.otheranimal celleng
dc.subject.otherBPAEC cell lineeng
dc.subject.othercalibrationeng
dc.subject.otherconfocal laser scanning microscopyeng
dc.subject.othercontrolled studyeng
dc.subject.otherimage reconstructioneng
dc.subject.othernonhumaneng
dc.subject.otheroptical resolutioneng
dc.subject.otherpoint spread functioneng
dc.subject.otherscanning electron microscopyeng
dc.subject.othersignal noise ratioeng
dc.subject.otherconfocal microscopyeng
dc.subject.otherlasereng
dc.subject.otherprocedureseng
dc.titlesimpleISM—A straight forward guide to upgrade from confocal to ISMeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccess
wgl.contributorIPHT
wgl.subjectMedizin, Gesundheitger
wgl.typeZeitschriftenartikelger
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