Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

dc.bibliographicCitation.firstPage3084
dc.bibliographicCitation.issue12
dc.bibliographicCitation.journalTitleBiomedicines : open access journaleng
dc.bibliographicCitation.volume10
dc.contributor.authorWolff, Christina M.
dc.contributor.authorKolb, Juergen F.
dc.contributor.authorBekeschus, Sander
dc.date.accessioned2023-02-21T06:32:55Z
dc.date.available2023-02-21T06:32:55Z
dc.date.issued2022
dc.description.abstractIn modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/11464
dc.identifier.urihttp://dx.doi.org/10.34657/10498
dc.language.isoeng
dc.publisherBasel : MDPI
dc.relation.doihttps://doi.org/10.3390/biomedicines10123084
dc.relation.essn2227-9059
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subject.ddc610
dc.subject.ddc570
dc.subject.otherapoptosiseng
dc.subject.otherkINPeneng
dc.subject.otherleukemiaeng
dc.subject.otherplasma medicineeng
dc.subject.otherreactive oxygen specieseng
dc.subject.otherROSeng
dc.titleCombined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fieldseng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccess
wgl.contributorINP
wgl.subjectMedizin, Gesundheitger
wgl.subjectBiowissenschaften/Biologieger
wgl.typeZeitschriftenartikelger
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