Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation
dc.bibliographicCitation.firstPage | 799 | eng |
dc.bibliographicCitation.issue | 2 | eng |
dc.bibliographicCitation.journalTitle | International Journal of Molecular Sciences | eng |
dc.bibliographicCitation.volume | 22 | eng |
dc.contributor.author | Peckys, Diana B. | |
dc.contributor.author | Gaa, Daniel | |
dc.contributor.author | Alansary, Dalia | |
dc.contributor.author | Niemeyer, Barbara A. | |
dc.contributor.author | de Jonge, Niels | |
dc.date.accessioned | 2021-07-02T09:10:44Z | |
dc.date.available | 2021-07-02T09:10:44Z | |
dc.date.issued | 2021 | |
dc.description.abstract | The Ca2+ selective channel ORAI1 and endoplasmic reticulum (ER)-resident STIM proteins form the core of the channel complex mediating store operated Ca2+ entry (SOCE). Using liquid phase electron microscopy (LPEM), the distribution of ORAI1 proteins was examined at rest and after SOCE-activation at nanoscale resolution. The analysis of over seven hundred thousand ORAI1 positions revealed a number of ORAI1 channels had formed STIM-independent distinct supra-molecular clusters. Upon SOCE activation and in the presence of STIM proteins, a fraction of ORAI1 assembled in micron-sized two-dimensional structures, such as the known puncta at the ER plasma membrane contact zones, but also in divergent structures such as strands, and ring-like shapes. Our results thus question the hypothesis that stochastically migrating single ORAI1 channels are trapped at regions containing activated STIM, and we propose instead that supra-molecular ORAI1 clusters fulfill an amplifying function for creating dense ORAI1 accumulations upon SOCE-activation. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/6206 | |
dc.identifier.uri | https://doi.org/10.34657/5253 | |
dc.language.iso | eng | eng |
dc.publisher | Basel : Molecular Diversity Preservation International | eng |
dc.relation.doi | https://doi.org/10.3390/ijms22020799 | |
dc.relation.essn | 1422-0067 | |
dc.relation.issn | 1661-6596 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 540 | eng |
dc.subject.other | calcium channel | eng |
dc.subject.other | protein clusters | eng |
dc.subject.other | membrane protein | eng |
dc.subject.other | liquid phase electron microscopy | eng |
dc.subject.other | single molecule | eng |
dc.subject.other | correlative microscopy | eng |
dc.title | Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | INM | eng |
wgl.subject | Chemie | eng |
wgl.type | Zeitschriftenartikel | eng |
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