CC BY 4.0 UnportedReuter, CorneliaUrban, MatthiasArnold, ManuelStranik, OndrejCsáki, AndreaFritzsche, Wolfgang2022-12-082022-12-082020https://oa.tib.eu/renate/handle/123456789/10521http://dx.doi.org/10.34657/9557Novel point-of-care compatible methods such as colorimetric assays have become increasingly important in the field of early pathogen detection. A simple and hand-held prototype device for carrying out DNA-amplification assay based on plasmonic nanoparticles in the colorimetric detection is presented. The low-cost device with two channels (sample and reference) consists of two spectrally different light emitting diodes (LEDs) for detection of the plasmon shift. The color change of the gold-nanoparticle-DNA conjugates caused by a salt-induced aggregation test is examined in particular. A specific and sensitive detection of the waterborne human pathogen Legionella pneumophila is demonstrated. This colorimetric assay, with a simple assay design and simple readout device requirements, can be monitored in real-time on-site. © 2020 by the authors.enghttps://creativecommons.org/licenses/by/4.0/600Colorimetric assayDNANoble-metal nanoparticlesNon-crosslinkingSensingArticle