CC BY-NC 3.0 UnportedCcorahua, RobertMoreno, SilviaGumz, HannesSahre, KarinVoit, BrigitteAppelhans, Dietmar2023-04-272023-04-272018https://oa.tib.eu/renate/handle/123456789/12120http://dx.doi.org/10.34657/11154Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (-20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor.enghttps://creativecommons.org/licenses/by-nc/3.0540Biological experimentsCryogenic freezingDispersing processFreeze-drying processFunctionalizedPH-responsivePolymersomesTherapeutic agentsFunctional polymersArticle