ACS AuthorChoiceXi, W.Schmidt, C.K.Sanchez, S.Gracias, D.H.Carazo-Salas, R.E.Jackson, S.P.Schmidt, O.G.2020-11-122020-11-122014https://doi.org/10.34657/4523https://oa.tib.eu/renate/handle/123456789/5894We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.enghttps://pubs.acs.org/page/policy/authorchoice_termsofuse.html6203D cell culture scaffoldchromosome segregation errorsmitosisNanomembranesrolled-up nanotechnologyspatial confinementCell cultureChromosomesNanostructures3-D cell cultureChromosome segregationmitosisNanomembranesSpatial confinementScaffolds (biology)artificial membranenanomaterialartificial membranechemistryHeLa cell linehumanmetaphaseHeLa CellsHumansMembranes, ArtificialMetaphaseNanostructuresArticle