CC BY 4.0 UnportedGandhirajan, Rajesh K.Endlich, NicoleBekeschus, Sander2021-12-072021-12-072021https://oa.tib.eu/renate/handle/123456789/7660https://doi.org/10.34657/6707Plasma technology has emerged as a promising tool in medicine that, however, requires not only efficacy but also toxicological assessments. Traditional cell culture systems are fast and economical, but they lack in vivo relevance; however, rodent models are highly complex and necessitate extended facilities. Zebrafish larvae bridge this gap, and many larvae can be analyzed in well plates in a single run, giving results in 1–2 days. Using the kINPen, we found plasma exposure to reduce hedging rates and viability in a dose-dependent manner, accompanied with an increase in reactive oxygen species and a decrease of glutathione in plasma-treated fish. Modest growth alterations were also observed. Altogether, zebrafish larvae constitute a fast, reliable, and relevant model for testing the toxicity of plasma sources.enghttps://creativecommons.org/licenses/by/4.0/530540glutathionekINPenreactive oxygen speciesROSArticle