CC BY 4.0 UnportedHalder, ParthoChen, Yi-chunBrauckhoff, JanineHofbauer, AloisDabauvalle, Marie-ChristineLewandrowski, UrsWinkler, ChristianeSickmann, AlbertBuchner, Erich2018-02-212019-06-282011https://doi.org/10.34657/1746https://oa.tib.eu/renate/handle/123456789/3690The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.application/pdfenghttps://creativecommons.org/licenses/by/4.0/610AntibodiesMonoclonalAntigensDrosophila ProteinsEps15 proteinDrosophilaNerve Tissue ProteinsArticle