CC BY 4.0 UnportedBarbotin, AurélienGaliani, SilviaUrbančič, IztokEggeling, ChristianBooth, Martin J.2020-01-032020-01-032019https://doi.org/10.34657/69https://oa.tib.eu/renate/handle/123456789/4798Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (z) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.enghttps://creativecommons.org/licenses/by/4.0/620STED-FCSmolecular diffusioncellsArticle