Argon Humidification Exacerbates Antimicrobial and Anti-MRSA kINPen Plasma Activity

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Date
2023
Volume
13
Issue
2
Journal Title
Title
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Publisher
Basel : MDPI
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Abstract

Gas plasma is a medical technology with antimicrobial properties. Its main mode of action is oxidative damage via reactive species production. The clinical efficacy of gas plasma-reduced bacterial burden has been shown to be hampered in some cases. Since the reactive species profile produced by gas plasma jets, such as the kINPen used in this study, are thought to determine antimicrobial efficacy, we screened an array of feed gas settings in different types of bacteria. Antimicrobial analysis was performed by single-cell analysis using flow cytometry. We identified humidified feed gas to mediate significantly greater toxicity compared to dry argon and many other gas plasma conditions. The results were confirmed by inhibition zone analysis on gas-plasma-treated microbial lawns grown on agar plates. Our results may have vital implications for clinical wound management and potentially enhance antimicrobial efficacy of medical gas plasma therapy in patient treatment.

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Keywords
Candida albicans, Escherichia coli, gas plasma technology, kINPen, plasma medicine, Pseudomonas aeruginosa, Pseudomonasspp, reactive oxygen species, ROS, Staphylococcus aureus, Staphylococcus epidermidis
Citation
Clemen, R., Singer, D., Skowski, H., & Bekeschus, S. (2023). Argon Humidification Exacerbates Antimicrobial and Anti-MRSA kINPen Plasma Activity. 13(2). https://doi.org//10.3390/life13020257
License
CC BY 4.0 Unported