A smart polymer for sequence-selective binding, pulldown, and release of DNA targets

dc.bibliographicCitation.firstPage369eng
dc.bibliographicCitation.issue1eng
dc.bibliographicCitation.journalTitleCommunications biologyeng
dc.bibliographicCitation.volume3eng
dc.contributor.authorKrieg, Elisha
dc.contributor.authorGupta, Krishna
dc.contributor.authorDahl, Andreas
dc.contributor.authorLesche, Mathias
dc.contributor.authorBoye, Susanne
dc.contributor.authorLederer, Albena
dc.contributor.authorShih, William M.
dc.date.accessioned2021-09-09T11:05:58Z
dc.date.available2021-09-09T11:05:58Z
dc.date.issued2020
dc.description.abstractSelective isolation of DNA is crucial for applications in biology, bionanotechnology, clinical diagnostics and forensics. We herein report a smart methanol-responsive polymer (MeRPy) that can be programmed to bind and separate single- as well as double-stranded DNA targets. Captured targets are quickly isolated and released back into solution by denaturation (sequence-agnostic) or toehold-mediated strand displacement (sequence-selective). The latter mode allows 99.8% efficient removal of unwanted sequences and 79% recovery of highly pure target sequences. We applied MeRPy for the depletion of insulin, glucagon, and transthyretin cDNA from clinical next-generation sequencing (NGS) libraries. This step improved the data quality for low-abundance transcripts in expression profiles of pancreatic tissues. Its low cost, scalability, high stability and ease of use make MeRPy suitable for diverse applications in research and clinical laboratories, including enhancement of NGS libraries, extraction of DNA from biological samples, preparative-scale DNA isolations, and sorting of DNA-labeled non-nucleic acid targets. © 2020, The Author(s).eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6768
dc.identifier.urihttps://doi.org/10.34657/5815
dc.language.isoengeng
dc.publisherLondon : Springer Natureeng
dc.relation.doihttps://doi.org/10.1038/s42003-020-1082-2
dc.relation.essn2399-3642
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc570eng
dc.subject.otherDNAeng
dc.subject.othermethanol-responsive polymer (MeRPy)eng
dc.subject.otherdouble-stranded DNA targetseng
dc.subject.othersingle-stranded DNA targetseng
dc.titleA smart polymer for sequence-selective binding, pulldown, and release of DNA targetseng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPFeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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