In search of a phosphorus dendrimer-based carrier of rose bengal: Tyramine linker limits fluorescent and phototoxic properties of a photosensitizer

dc.bibliographicCitation.firstPage4456eng
dc.bibliographicCitation.issue12eng
dc.bibliographicCitation.volume21eng
dc.contributor.authorSztandera, Krzysztof
dc.contributor.authorMarcinkowska, Monika
dc.contributor.authorGorzkiewicz, Michał
dc.contributor.authorJanaszewska, Anna
dc.contributor.authorLaurent, Regis
dc.contributor.authorZabłocka, Maria
dc.contributor.authorMignani, Serge
dc.contributor.authorMajoral, Jean Pierre
dc.contributor.authorKlajnert-Maculewicz, Barbara
dc.date.accessioned2021-11-05T09:32:18Z
dc.date.available2021-11-05T09:32:18Z
dc.date.issued2020
dc.description.abstractPhotodynamic therapy (PDT) is a skin cancer treatment alternative to chemotherapy and radiotherapy. This method exploits three elements: a phototoxic compound (photosensitizer), light source and oxygen. Upon irradiation by light of a specific wavelength, the photosensitizer generates reactive oxygen species triggering the cascade of reactions leading to cell death. The positive therapeutic effect of PDT may be limited due to low solubility, low tumor specificity and inefficient cellular uptake of photosensitizers. A promising approach to overcome these obstacles involves the use of nanocarrier systems. The aim of this initial study was to determine the potential of the application of phosphorus dendrimers as carriers of a photosensitizer—rose bengal (RB). The primary goal involved the synthesis and in vitro studies of covalent drug–dendrimer conjugates. Our approach allowed us to obtain RB–dendrimer conjugates with the use of tyramine as an aromatic linker between the carrier and the drug. The compounds were characterized by FT-IR,1H NMR,13C NMR,31P NMR, size and zeta potential measurements and spectrofluorimetric analysis. The dialysis to check the drug release from the conjugate, flow cytometry to specify intracellular uptake, and singlet oxygen generation assay were also applied. Finally, we used MTT assay to determine the biological activity of the tested compounds. The results of our experiments indicate that the conjugation of RB to phosphorus dendrimers via the tyramine linker decreases photodynamic activity of RB. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/7188
dc.identifier.urihttps://doi.org/10.34657/6235
dc.language.isoengeng
dc.publisherBasel : Molecular Diversity Preservation Internationaleng
dc.relation.doihttps://doi.org/10.3390/ijms21124456
dc.relation.essn1422-0067
dc.relation.ispartofseriesInternational journal of molecular sciences 21 (2020), Nr. 12eng
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subjectPhosphorus dendrimerseng
dc.subjectPhotodynamic therapyeng
dc.subjectRose bengal (RB)eng
dc.subjectSkin cancereng
dc.subject.ddc570eng
dc.subject.ddc540eng
dc.titleIn search of a phosphorus dendrimer-based carrier of rose bengal: Tyramine linker limits fluorescent and phototoxic properties of a photosensitizereng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleInternational journal of molecular scienceseng
tib.accessRightsopenAccesseng
wgl.contributorIPFeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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