Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

dc.bibliographicCitation.firstPage2291eng
dc.bibliographicCitation.issue7eng
dc.bibliographicCitation.volume21eng
dc.contributor.authorHaralambiev, Lyubomir
dc.contributor.authorNitsch, Andreas
dc.contributor.authorJacoby, Josephine M.
dc.contributor.authorStrakeljahn, Silas
dc.contributor.authorBekeschus, Sander
dc.contributor.authorMustea, Alexander
dc.contributor.authorEkkernkamp, Axel
dc.contributor.authorStope, Matthias B.
dc.date.accessioned2021-11-03T07:24:01Z
dc.date.available2021-11-03T07:24:01Z
dc.date.issued2020
dc.description.abstractChondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G-and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/7166
dc.identifier.urihttps://doi.org/10.34657/6213
dc.language.isoengeng
dc.publisherBasel : Molecular Diversity Preservation Internationaleng
dc.relation.doihttps://doi.org/10.3390/ijms21072291
dc.relation.essn1422-0067
dc.relation.ispartofseriesInternational journal of molecular sciences 21 (2020), Nr. 7eng
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subjectChondrosarcomaeng
dc.subjectCold atmospheric plasmaeng
dc.subjectGrowth inhibitioneng
dc.subjectMembrane functionalityeng
dc.subject.ddc570eng
dc.subject.ddc540eng
dc.titleCold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeabilityeng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleInternational journal of molecular scienceseng
tib.accessRightsopenAccesseng
wgl.contributorINPeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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