A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits

dc.bibliographicCitation.firstPage119537eng
dc.bibliographicCitation.volume225eng
dc.contributor.authorBellmann, Jessica
dc.contributor.authorGoswami, Ruchi Y.
dc.contributor.authorGirardo, Salvatore
dc.contributor.authorRein, Nelly
dc.contributor.authorHosseinzadeh, Zohreh
dc.contributor.authorHicks, Michael R.
dc.contributor.authorBusskamp, Volker
dc.contributor.authorPyle, April D.
dc.contributor.authorWerner, Carsten
dc.contributor.authorSterneckert, Jared
dc.date.accessioned2021-09-09T08:37:03Z
dc.date.available2021-09-09T08:37:03Z
dc.date.issued2019
dc.description.abstractNeuromuscular circuits (NMCs) are vital for voluntary movement, and effective models of NMCs are needed to understand the pathogenesis of, as well as to identify effective treatments for, multiple diseases, including Duchenne's muscular dystrophy and amyotrophic lateral sclerosis. Microfluidics are ideal for recapitulating the central and peripheral compartments of NMCs, but myotubes often detach before functional NMCs are formed. In addition, microfluidic systems are often limited to a single experimental unit, which significantly limits their application in disease modeling and drug discovery. Here, we developed a microfluidic platform (MFP) containing over 100 experimental units, making it suitable for medium-throughput applications. To overcome detachment, we incorporated a reactive polymer surface allowing customization of the environment to culture different cell types. Using this approach, we identified conditions that enable long-term co-culture of human motor neurons and myotubes differentiated from human induced pluripotent stem cells inside our MFP. Optogenetics demonstrated the formation of functional NMCs. Furthermore, we developed a novel application of the rabies tracing assay to efficiently identify NMCs in our MFP. Therefore, our MFP enables large-scale generation and quantification of functional NMCs for disease modeling and pharmacological drug targeting. © 2019 The Authorseng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6765
dc.identifier.urihttps://doi.org/10.34657/5812
dc.language.isoengeng
dc.publisherAmsterdam [u.a.] : Elsevier Scienceeng
dc.relation.doihttps://doi.org/10.1016/j.biomaterials.2019.119537
dc.relation.essn1878-5905
dc.relation.ispartofseriesBiomaterials : biomaterials reviews online 225 (2019)eng
dc.relation.issn0142-9612
dc.rights.licenseCC BY-NC-ND 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectMicrofluidicseng
dc.subjectMotor uniteng
dc.subjectNeuromuscular circuiteng
dc.subjectpoly(ethylene-alt-maleic anhydride)eng
dc.subjectRabies viral tracingeng
dc.subjectSkeletal muscleeng
dc.subject.ddc570eng
dc.titleA customizable microfluidic platform for medium-throughput modeling of neuromuscular circuitseng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleBiomaterials : biomaterials reviews onlineeng
tib.accessRightsopenAccesseng
wgl.contributorIPFeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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