More Favorable Palmitic Acid Over Palmitoleic Acid Modification of Wnt3 Ensures Its Localization and Activity in Plasma Membrane Domains

dc.bibliographicCitation.firstPage281eng
dc.bibliographicCitation.volume7eng
dc.contributor.authorAzbazdar, Yagmur
dc.contributor.authorOzalp, Ozgun
dc.contributor.authorSezgin, Erdinc
dc.contributor.authorVeerapathiran, Sapthaswaran
dc.contributor.authorDuncan, Anna L.
dc.contributor.authorSansom, Mark S.P.
dc.contributor.authorEggeling, Christian
dc.contributor.authorWohland, Thorsten
dc.contributor.authorKaraca, Ezgi
dc.contributor.authorOzhan, Gunes
dc.date.accessioned2021-10-28T13:26:47Z
dc.date.available2021-10-28T13:26:47Z
dc.date.issued2019
dc.description.abstractWhile the lateral organization of plasma membrane components has been shown to control binding of Wnt ligands to their receptors preferentially in the ordered membrane domains, the role of posttranslational lipid modification of Wnt on this selective binding is unknown. Here, we identify that the canonical Wnt is presumably acylated by palmitic acid, a saturated 16-carbon fatty acid, at a conserved serine residue. Acylation of Wnt3 is dispensable for its secretion and binding to Fz8 while it is essential for Wnt3's proper binding and domain-like diffusion in the ordered membrane domains. We further unravel that non-palmitoylated Wnt3 is unable to activate Wnt/β-catenin signaling either in zebrafish embryos or in mammalian cells. Based on these results, we propose that the lipidation of canonical Wnt, presumably by a saturated fatty acid, determines its competence in interacting with the receptors in the appropriate domains of the plasma membrane, ultimately keeping the signaling activity under control. © Copyright © 2019 Azbazdar, Ozalp, Sezgin, Veerapathiran, Duncan, Sansom, Eggeling, Wohland, Karaca and Ozhan.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/7136
dc.identifier.urihttps://doi.org/10.34657/6183
dc.language.isoengeng
dc.publisherLausanne : Frontiers Mediaeng
dc.relation.doihttps://doi.org/10.3389/fcell.2019.00281
dc.relation.essn2296-634X
dc.relation.ispartofseriesFrontiers in Cell and Developmental Biology 7 (2019)eng
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subjectacylationeng
dc.subjectlipid rafteng
dc.subjectordered plasma membrane domaineng
dc.subjectpalmitoylationeng
dc.subjectstructural modelingeng
dc.subjectWnt/β-catenin pathwayeng
dc.subject.ddc570eng
dc.titleMore Favorable Palmitic Acid Over Palmitoleic Acid Modification of Wnt3 Ensures Its Localization and Activity in Plasma Membrane Domainseng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleFrontiers in Cell and Developmental Biologyeng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectBiowissensschaften/Biologieeng
wgl.typeZeitschriftenartikeleng
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