Poly (hexamethylene biguanide), adsorbed onto Ti-Al-V alloys, kills slime-producing Staphylococci and Pseudomonas aeruginosa without inhibiting SaOs-2 cell differentiation
dc.bibliographicCitation.firstPage | 1801 | eng |
dc.bibliographicCitation.issue | 5 | eng |
dc.bibliographicCitation.journalTitle | Journal of biomedical materials research : Part B, Applied biomaterials | eng |
dc.bibliographicCitation.lastPage | 1813 | eng |
dc.bibliographicCitation.volume | 108 | eng |
dc.contributor.author | Hornschuh, Melanie | |
dc.contributor.author | Zwicker, Paula | |
dc.contributor.author | Schmidt, Thomas | |
dc.contributor.author | Finke, Birgit | |
dc.contributor.author | Kramer, Axel | |
dc.contributor.author | Müller, Gerald | |
dc.date.accessioned | 2021-11-03T08:10:52Z | |
dc.date.available | 2021-11-03T08:10:52Z | |
dc.date.issued | 2020 | |
dc.description.abstract | Antimicrobial coating of implant material with poly(hexamethylene biguanide) hydrochloride (PHMB) may be an eligible method for preventing implant-associated infections. In the present study, an antibacterial effective amount of PHMB is adsorbed on the surface of titanium alloy after simple chemical pretreatment. Either oxidation with 5% H2O2 for 24 hr or processing for 2 hr in 5 M NaOH provides the base for the subsequent formation of a relatively stable self-assembled PHMB layer. Compared with an untreated control group, adsorbed PHMB produces no adverse effects on SaOs-2 cells within 48 hr cell culture, but promotes the initial attachment and spreading of the osteoblasts within 15 min. Specimens were inoculated with slime-producing bacteria to simulate a perioperative infection. Adsorbed PHMB reacts bactericidally against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa after surface contact. Adhered SaOs-2 cells differentiate and produce alkaline phosphatase and deposit calcium within 4 days in a mineralization medium on PHMB-coated Ti6Al4V surfaces, which have been precontaminated with S. epidermidis. The presented procedures provide a simple method for generating biocompatibly and antimicrobially effective implant surfaces that may be clinically important. © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/7169 | |
dc.identifier.uri | https://doi.org/10.34657/6216 | |
dc.language.iso | eng | eng |
dc.publisher | Hoboken, NJ : Wiley | eng |
dc.relation.doi | https://doi.org/10.1002/jbm.b.34522 | |
dc.relation.essn | 1552-4981 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 570 | eng |
dc.subject.other | contact killing surface | eng |
dc.subject.other | PHMB adsorption | eng |
dc.subject.other | SaOs-2 cell differentiation | eng |
dc.subject.other | surface modification | eng |
dc.subject.other | titanium alloys | eng |
dc.title | Poly (hexamethylene biguanide), adsorbed onto Ti-Al-V alloys, kills slime-producing Staphylococci and Pseudomonas aeruginosa without inhibiting SaOs-2 cell differentiation | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | INP | eng |
wgl.subject | Biowissensschaften/Biologie | eng |
wgl.type | Zeitschriftenartikel | eng |
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