Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes-in-Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation
dc.bibliographicCitation.firstPage | 2005749 | eng |
dc.bibliographicCitation.issue | 7 | eng |
dc.bibliographicCitation.volume | 17 | eng |
dc.contributor.author | Wen, Ping | |
dc.contributor.author | Wang, Xueyi | |
dc.contributor.author | Moreno, Silvia | |
dc.contributor.author | Boye, Susanne | |
dc.contributor.author | Voigt, Dagmar | |
dc.contributor.author | Voit, Brigitte | |
dc.contributor.author | Huang, Xin | |
dc.contributor.author | Appelhans, Dietmar | |
dc.date.accessioned | 2021-12-15T08:32:44Z | |
dc.date.available | 2021-12-15T08:32:44Z | |
dc.date.issued | 2021 | |
dc.description.abstract | The eukaryotic cell is a smart compartment containing an outer permeable membrane, a cytoskeleton, and functional organelles, presenting part structures for life. The integration of membrane-containing artificial organelles (=polymersomes) into a large microcompartment is a key step towards the establishment of exquisite cellular biomimetics with different membrane properties. Herein, an efficient way to construct a hierarchical multicompartment composed of a hydrogel-filled proteinosome hybrid structure with an outer homogeneous membrane, a smart cytoskeleton-like scaffold, and polymersomes is designed. Specially, this hybrid structure creates a micro-environment for pH-responsive polymersomes to execute a desired substance transport upon response to biological stimuli. Within the dynamic pH-stable skeleton of the protein hydrogels, polymersomes with loaded PEGylated insulin biomacromolecules demonstrate a pH-responsive reversible swelling-deswelling and a desirable, on-demand cargo release which is induced by the enzymatic oxidation of glucose to gluconic acid. This stimulus responsive behavior is realized by tunable on/off states through protonation of the polymersomes membrane under the enzymatic reaction of glucose oxidase, integrated in the skeleton of protein hydrogels. The integration of polymersomes-based hybrid structure into the proteinosome compartment and the stimuli-response on enzyme reactions fulfills the requirements of eukaryotic cell biomimetics in complex architectures and allows mimicking cellular transportation processes. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/7755 | |
dc.identifier.uri | https://doi.org/10.34657/6802 | |
dc.language.iso | eng | eng |
dc.publisher | Weinheim : Wiley-VCH | eng |
dc.relation.doi | https://doi.org/10.1002/smll.202005749 | |
dc.relation.essn | 1613-6829 | |
dc.relation.ispartofseries | Small : nano micro 17 (2021), Nr. 7 | eng |
dc.rights.license | CC BY-NC-ND 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | eng |
dc.subject | biological responses | eng |
dc.subject | cell engineering | eng |
dc.subject | multicompartments | eng |
dc.subject | polymersomes | eng |
dc.subject | proteinosomes | eng |
dc.subject.ddc | 570 | eng |
dc.subject.ddc | 620 | eng |
dc.title | Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes-in-Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation | eng |
dc.type | article | eng |
dc.type | Text | eng |
dcterms.bibliographicCitation.journalTitle | Small : nano micro | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPF | eng |
wgl.subject | Biowissensschaften/Biologie | eng |
wgl.type | Zeitschriftenartikel | eng |
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