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    Uncertainty in the measurement of indoor temperature and humidity in naturally ventilated dairy buildings as influenced by measurement technique and data variability
    (Amsterdam : Elsevier, 2017) Hempel, Sabrina; König, Marcel; Menz, Christoph; Janke, David; Amon, Barbara; Banhazi, Thomas M.; Estellés, Fernando; Amon, Thomas
    The microclimatic conditions in dairy buildings affect animal welfare and gaseous emissions. Measurements are highly variable due to the inhomogeneous distribution of heat and humidity sources (related to farm management) and the turbulent inflow (associated with meteorologic boundary conditions). The selection of the measurement strategy (number and position of the sensors) and the analysis methodology adds to the uncertainty of the applied measurement technique. To assess the suitability of different sensor positions, in situations where monitoring in the direct vicinity of the animals is not possible, we collected long-term data in two naturally ventilated dairy barns in Germany between March 2015 and April 2016 (horizontal and vertical profiles with 10 to 5 min temporal resolution). Uncertainties related to the measurement setup were assessed by comparing the device outputs under lab conditions after the on-farm experiments. We found out that the uncertainty in measurements of relative humidity is of particular importance when assessing heat stress risk and resulting economic losses in terms of temperature-humidity index. Measurements at a height of approximately 3 m–3.5 m turned out to be a good approximation for the microclimatic conditions in the animal occupied zone (including the air volume close to the emission active zone). However, further investigation along this cross-section is required to reduce uncertainties related to the inhomogeneous distribution of humidity. In addition, a regular sound cleaning (and if possible recalibration after few months) of the measurement devices is crucial to reduce the instrumentation uncertainty in long-term monitoring of relative humidity in dairy barns. © 2017 The Authors
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    Effect of Fans’ Placement on the Indoor Thermal Environment of Typical Tunnel-Ventilated Multi-Floor Pig Buildings Using Numerical Simulation
    (Basel : MDPI AG, 2022) Wang, Xiaoshuai; Cao, Mengbing; Hu, Feiyue; Yi, Qianying; Amon, Thomas; Janke, David; Xie, Tian; Zhang, Guoqiang; Wang, Kaiying
    An increasing number of large pig farms are being built in multi-floor pig buildings (MFPBs) in China. Currently, the ventilation system of MFPB varies greatly and lacks common standards. This work aims to compare the ventilation performance of three popular MFPB types with different placement of fans using the Computational Fluid Dynamics (CFD) technique. After being validated with field-measured data, the CFD models were extended to simulate the air velocity, air temperature, humidity, and effective temperature of the three MFPBs. The simulation results showed that the ventilation rate of the building with outflowing openings in the endwall and fans installed on the top of the shaft was approximately 25% less than the two buildings with fans installed on each floor. The ventilation rate of each floor increased from the first to the top floor for both buildings with a shaft, while no significant difference was observed in the building without a shaft. Increasing the shaft’s width could mitigate the variation in the ventilation rate of each floor. The effective temperature distribution at the animal level was consistent with the air velocity distribution. Therefore, in terms of the indoor environmental condition, the fans were recommended to be installed separately on each floor.
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    A Parametric Model for Local Air Exchange Rate of Naturally Ventilated Barns
    (Basel : MDPI AG, 2021) Doumbia, E. Moustapha; Janke, David; Yi, Qianying; Prinz, Alexander; Amon, Thomas; Kriegel, Martin; Hempel, Sabrina
    With an increasing number of naturally ventilated dairy barns (NVDBs), the emission of ammonia and greenhouse gases into the surrounding environment is expected to increase as well. It is very challenging to accurately determine the amount of gases released from a NVDB on-farm. Moreover, control options for the micro-climate to increase animal welfare are limited in an NVDB at present. Both issues are due to the complexity of the NVDB micro-environment, which is subject to temporal (such as wind direction and temperature) and spatial (such as openings and animals acting as airflow obstacles) fluctuations. The air exchange rate (AER) is one of the most valuable evaluation entities, since it is directly related to the gas emission rate and animal welfare. In this context, our study determined the general and local AERs of NVDBs of different shapes under diverse airflow conditions. Previous works identified main influencing parameters for the general AER and mathematically linked them together to predict the AER of the barn as a whole. The present research study is a continuation and extension of previous studies about the determination of AER. It provides new insights into the influence of convection flow regimes. In addition, it goes further in precision by determining the local AERs, depending on the position of the considered volume inside the barn. After running several computational fluid dynamics (CFD) simulations, we used the statistical tool of general linear modeling in order to identify quantitative relationships between the AER and the following five influencing parameters, the length/width ratio of the barn, the side opening configuration, the airflow temperature, magnitude and incoming direction. The work succeeded in taking the temperature into account as a further influencing parameter in the model and, thus, for the first time, in analysing the effect of the different types of flow convection in this context. The resulting equations predict the barn AER with an R2 equals 0.98 and the local AER with a mean R2 equals around 0.87. The results go a step further in the precise determination of the AER of NVDB and, therefore, are of fundamental importance for a better and deeper understanding of the interaction between the driving forces of AER in NVDB.
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    Heat stress risk in European dairy cattle husbandry under different climate change scenarios – uncertainties and potential impacts
    (Göttingen : Copernicus, 2019) Hempel, Sabrina; Menz, Christoph; Pinto, Severino; Galán, Elena; Janke, David; Estellés, Fernando; Müschner-Siemens, Theresa; Wang, Xiaoshuai; Heinicke, Julia; Zhang, Guoqiang; Amon, Barbara; del Prado, Agustín; Amon, Thomas
    In the last decades, a global warming trend was observed. Along with the temperature increase, modifications in the humidity and wind regime amplify the regional and local impacts on livestock husbandry. Direct impacts include the occurrence of climatic stress conditions. In Europe, cows are economically highly relevant and are mainly kept in naturally ventilated buildings that are most susceptible to climate change. The high-yielding cows are particularly vulnerable to heat stress. Modifications in housing management are the main measures taken to improve the ability of livestock to cope with these conditions. Measures are typically taken in direct reaction to uncomfortable conditions instead of in anticipation of a long-term risk for climatic stress. Measures that balance welfare, environmental and economic issues are barely investigated in the context of climate change and are thus almost not available for commercial farms. Quantitative analysis of the climate change impacts on animal welfare and linked economic and environmental factors is rare. Therefore, we used a numerical modeling approach to estimate the future heat stress risk in such dairy cattle husbandry systems. The indoor climate was monitored inside three reference barns in central Europe and the Mediterranean regions. An artificial neuronal network (ANN) was trained to relate the outdoor weather conditions provided by official meteorological weather stations to the measured indoor microclimate. Subsequently, this ANN model was driven by an ensemble of regional climate model projections with three different greenhouse gas concentration scenarios. For the evaluation of the heat stress risk, we considered the number and duration of heat stress events. Based on the changes in the heat stress events, various economic and environmental impacts were estimated. The impacts of the projected increase in heat stress risk varied among the barns due to different locations and designs as well as the anticipated climate change (considering different climate models and future greenhouse gas concentrations). There was an overall increasing trend in number and duration of heat stress events. At the end of the century, the number of annual stress events can be expected to increase by up to 2000, while the average duration of the events increases by up to 22 h compared to the end of the last century. This implies strong impacts on economics, environment and animal welfare and an urgent need for mid-term adaptation strategies. We anticipated that up to one-tenth of all hours of a year, correspondingly one-third of all days, will be classified as critical heat stress conditions. Due to heat stress, milk yield may decrease by about 2.8 % relative to the present European milk yield, and farmers may expect financial losses in the summer season of about 5.4 % of their monthly income. In addition, an increasing demand for emission reduction measures must be expected, as an emission increase of about 16 Gg of ammonia and 0.1 Gg of methane per year can be expected under the anticipated heat stress conditions. The cattle respiration rate increases by up to 60 %, and the standing time may be prolonged by 1 h. This causes health issues and increases the probability of medical treatments. The various impacts imply feedback loops in the climate system which are presently underexplored. Hence, future in-depth studies on the different impacts and adaptation options at different stress levels are highly recommended.
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    Airborne bacterial emission fluxes from manure-fertilized agricultural soil
    (Oxford : Wiley-Blackwell, 2020) Thiel, Nadine; Münch, Steffen; Behrens, Wiebke; Junker, Vera; Faust, Matthias; Biniasch, Oliver; Kabelitz, Tina; Siller, Paul; Boedeker, Christian; Schumann, Peter; Roesler, Uwe; Amon, Thomas; Schepanski, Kerstin; Funk, Roger; Nübel, Ulrich
    This is the first study to quantify the dependence on wind velocity of airborne bacterial emission fluxes from soil. It demonstrates that manure bacteria get aerosolized from fertilized soil more easily than soil bacteria, and it applies bacterial genomic sequencing for the first time to trace environmental faecal contamination back to its source in the chicken barn. We report quantitative, airborne emission fluxes of bacteria during and following the fertilization of agricultural soil with manure from broiler chickens. During the fertilization process, the concentration of airborne bacteria culturable on blood agar medium increased more than 600 000-fold, and 1 m3 of air carried 2.9 × 105 viable enterococci, i.e. indicators of faecal contamination which had been undetectable in background air samples. Trajectory modelling suggested that atmospheric residence times and dispersion pathways were dependent on the time of day at which fertilization was performed. Measurements in a wind tunnel indicated that airborne bacterial emission fluxes from freshly fertilized soil under local climatic conditions on average were 100-fold higher than a previous estimate of average emissions from land. Faecal bacteria collected from soil and dust up to seven weeks after fertilization could be traced to their origins in the poultry barn by genomic sequencing. Comparative analyses of 16S rRNA gene sequences from manure, soil and dust showed that manure bacteria got aerosolized preferably, likely due to their attachment to low-density manure particles. Our data show that fertilization with manure may cause substantial increases of bacterial emissions from agricultural land. After mechanical incorporation of manure into soil, however, the associated risk of airborne infection is low.
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    The Role of Streptococcus spp. in Bovine Mastitis
    (Basel : MDPI, 2021) Kabelitz, Tina; Aubry, Etienne; van Vorst, Kira; Amon, Thomas; Fulde, Marcus
    The Streptococcus genus belongs to one of the major pathogen groups inducing bovine mastitis. In the dairy industry, mastitis is the most common and costly disease. It not only negatively impacts economic profit due to milk losses and therapy costs, but it is an important animal health and welfare issue as well. This review describes a classification, reservoirs, and frequencies of the most relevant Streptococcus species inducing bovine mastitis (S. agalactiae, S. dysgalactiae and S. uberis). Host and environmental factors influencing mastitis susceptibility and infection rates will be discussed, because it has been indicated that Streptococcus herd prevalence is much higher than mastitis rates. After infection, we report the sequence of cow immune reactions and differences in virulence factors of the main Streptococcus species. Different mastitis detection techniques together with possible conventional and alternative therapies are described. The standard approach treating streptococcal mastitis is the application of ß-lactam antibiotics. In streptococci, increased antimicrobial resistance rates were identified against enrofloxacin, tetracycline, and erythromycin. At the end, control and prevention measures will be considered, including vaccination, hygiene plan, and further interventions. It is the aim of this review to estimate the contribution and to provide detailed knowledge about the role of the Streptococcus genus in bovine mastitis.
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    Effect of two cooling frequencies on respiration rate in lactating dairy cows under hot and humid climate conditions
    (Warsaw : De Gruyter Open, 2019) Pinto, Severino; Hoffmann, Gundula; Ammon, Christian; Heuwieser, Wolfgang; Levit, Harel; Halachmi, Ilan; Amon, Thomas
    The aim of this study was to evaluate the effects of evaporative cooling at two different frequen-cies per day on the respiration rate (rr) of lactating dairy cows, considering cow-related factors. twenty multiparous israeli holstein dairy cows housed in a naturally ventilated cowshed were di-vided randomly into two treatment groups. the cows of both groups were exposed to 3 or 8 cooling sessions per day (3xcool vs. 8xcool, respectively). the rr was observed hourly, with a maximum of 12 measurements per day. Body posture (standing vs. lying) was simultaneously documented. milk yield was recorded daily. coat color was determined from a digital photograph. the rr of standing and lying cows was lower in the 8xcool group (60.2 and 51.6 breaths per min (bpm), re-spectively) than in the 3xcool group (73.1 and 65.6 bpm, respectively). For each increment of five kilograms of milk produced, rr increased by one bpm, and the rr of cows in early days in milk (dim) was 12.3 bpm higher than that of cows in late dim. in conclusion, eight cooling sessions per day instead of three lead to a rr abatement in heat-stressed cows under hot conditions, and cow-related factors directly impact the rr during heat stress assessment
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    Airflow Characteristics Downwind a Naturally Ventilated Pig Building with a Roofed Outdoor Exercise Yard and Implications on Pollutant Distribution
    (Basel : MDPI AG, 2020) Yi, Qianying; Janke, David; Thormann, Lars; Zhang, Guoqiang; Amon, Barbara; Hempel, Sabrina; Nosek, Štěpán; Hartung, Eberhard; Amon, Thomas
    The application of naturally ventilated pig buildings (NVPBs) with outdoor exercise yards is on the rise mainly due to animal welfare considerations, while the issue of emissions from the buildings to the surrounding environment is important. Since air pollutants are mainly transported by airflow, the knowledge on the airflow characteristics downwind the building is required. The objective of this research was to investigate airflow properties downwind of a NVPB with a roofed outdoor exercise yard for roof slopes of 5°, 15°, and 25°. Air velocities downwind a 1:50 scaled NVPB model were measured using a Laser Doppler Anemometer in a large boundary layer wind tunnel. A region with reduced mean air velocities was found along the downwind side of the building with a distance up to 0.5 m (i.e., 3.8 times building height), in which the emission concentration might be high. Additional air pollutant treatment technologies applied in this region might contribute to emission mitigation effectively. Furthermore, a wake zone with air recirculation was observed in this area. A smaller roof slope (i.e., 5° slope) resulted in a higher and shorter wake zone and thus a shorter air pollutant dispersion distance.
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    Supervised Machine Learning to Assess Methane Emissions of a Dairy Building with Natural Ventilation
    (Basel : MDPI, 2020) Hempel, Sabrina; Adolphs, Julian; Landwehr, Niels; Willink, Dilya; Janke, David; Amon, Thomas
    A reliable quantification of greenhouse gas emissions is a basis for the development of adequate mitigation measures. Protocols for emission measurements and data analysis approaches to extrapolate to accurate annual emission values are a substantial prerequisite in this context. We systematically analyzed the benefit of supervised machine learning methods to project methane emissions from a naturally ventilated cattle building with a concrete solid floor and manure scraper located in Northern Germany. We took into account approximately 40 weeks of hourly emission measurements and compared model predictions using eight regression approaches, 27 different sampling scenarios and four measures of model accuracy. Data normalization was applied based on median and quartile range. A correlation analysis was performed to evaluate the influence of individual features. This indicated only a very weak linear relation between the methane emission and features that are typically used to predict methane emission values of naturally ventilated barns. It further highlighted the added value of including day-time and squared ambient temperature as features. The error of the predicted emission values was in general below 10%. The results from Gaussian processes, ordinary multilinear regression and neural networks were least robust. More robust results were obtained with multilinear regression with regularization, support vector machines and particularly the ensemble methods gradient boosting and random forest. The latter had the added value to be rather insensitive against the normalization procedure. In the case of multilinear regression, also the removal of not significantly linearly related variables (i.e., keeping only the day-time component) led to robust modeling results. We concluded that measurement protocols with 7 days and six measurement periods can be considered sufficient to model methane emissions from the dairy barn with solid floor with manure scraper, particularly when periods are distributed over the year with a preference for transition periods. Features should be normalized according to median and quartile range and must be carefully selected depending on the modeling approach.
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    Opening Size E ects on Airflow Pattern and Airflow Rate of a Naturally Ventilated Dairy Building : A CFD Study
    (Basel : MDPI, 2020) Saha, Chayan Kumer; Yi, Qianying; Janke, David; Hempel, Sabrina; Amon, Barbara; Amon, Thomas
    Airflow inside naturally ventilated dairy (NVD) buildings is highly variable and difficult to understand due to the lack of precious measuring techniques with the existing methods. Computational fluid dynamics (CFD) was applied to investigate the effect of different seasonal opening combinations of an NVD building on airflow patterns and airflow rate inside the NVD building as an alternative to full scale and scale model experiments. ANSYS 2019R2 was used for creating model geometry, meshing, and simulation. Eight ventilation opening combinations and 10 different reference air velocities were used for the series of simulation. The data measured in a large boundary layer wind tunnel using a 1:100 scale model of the NVD building was used for CFD model validation. The results show that CFD using standard k-ε turbulence model was capable of simulating airflow in and outside of the NVD building. Airflow patterns were different for different opening scenarios at the same external wind speed, which may affect cow comfort and gaseous emissions. Guiding inlet air by controlling openings may ensure animal comfort and minimize emissions. Non-isothermal and transient simulations of NVD buildings should be carried out for better understanding of airflow patterns.