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search.filters.applied.f.access: openAccess × Author: Amon, Thomas × Type: Text × WGL Institute: TROPOS ×

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    Airborne bacterial emission fluxes from manure-fertilized agricultural soil
    (Oxford : Wiley-Blackwell, 2020) Thiel, Nadine; Münch, Steffen; Behrens, Wiebke; Junker, Vera; Faust, Matthias; Biniasch, Oliver; Kabelitz, Tina; Siller, Paul; Boedeker, Christian; Schumann, Peter; Roesler, Uwe; Amon, Thomas; Schepanski, Kerstin; Funk, Roger; Nübel, Ulrich
    This is the first study to quantify the dependence on wind velocity of airborne bacterial emission fluxes from soil. It demonstrates that manure bacteria get aerosolized from fertilized soil more easily than soil bacteria, and it applies bacterial genomic sequencing for the first time to trace environmental faecal contamination back to its source in the chicken barn. We report quantitative, airborne emission fluxes of bacteria during and following the fertilization of agricultural soil with manure from broiler chickens. During the fertilization process, the concentration of airborne bacteria culturable on blood agar medium increased more than 600 000-fold, and 1 m3 of air carried 2.9 × 105 viable enterococci, i.e. indicators of faecal contamination which had been undetectable in background air samples. Trajectory modelling suggested that atmospheric residence times and dispersion pathways were dependent on the time of day at which fertilization was performed. Measurements in a wind tunnel indicated that airborne bacterial emission fluxes from freshly fertilized soil under local climatic conditions on average were 100-fold higher than a previous estimate of average emissions from land. Faecal bacteria collected from soil and dust up to seven weeks after fertilization could be traced to their origins in the poultry barn by genomic sequencing. Comparative analyses of 16S rRNA gene sequences from manure, soil and dust showed that manure bacteria got aerosolized preferably, likely due to their attachment to low-density manure particles. Our data show that fertilization with manure may cause substantial increases of bacterial emissions from agricultural land. After mechanical incorporation of manure into soil, however, the associated risk of airborne infection is low.
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    Agricultural fertilization with poultry manure results in persistent environmental contamination with the pathogen Clostridioides difficile
    (Oxford [u.a.] : Blackwell, 2021) Frentrup, Martinique; Thiel, Nadine; Junker, Vera; Behrens, Wiebke; Münch, Steffen; Siller, Paul; Kabelitz, Tina; Faust, Matthias; Indra, Alexander; Baumgartner, Stefanie; Schepanski, Kerstin; Amon, Thomas; Roesler, Uwe; Funk, Roger; Nübel, Ulrich
    During a field experiment applying broiler manure for fertilization of agricultural land, we detected viable Clostridioides (also known as Clostridium) difficile in broiler faeces, manure, dust and fertilized soil. A large diversity of toxigenic C. difficile isolates was recovered, including PCR ribotypes common from human disease. Genomic relatedness of C. difficile isolates from dust and from soil, recovered more than 2 years after fertilization, traced their origins to the specific chicken farm that had delivered the manure. We present evidence of long-term contamination of agricultural soil with manure-derived C. difficile and demonstrate the potential for airborne dispersal of C. difficile through dust emissions during manure application. Clostridioides genome sequences virtually identical to those from manure had been recovered from chicken meat and from human infections in previous studies, suggesting broiler-associated C. difficile are capable of zoonotic transmission.
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    On the feasibility of using open source solvers for the simulation of a turbulent air flow in a dairy barn
    (Amsterdam [u.a.] : Elsevier, 2020) Janke, David; Caiazzo, Alfonso; Ahmed, Naveed; Alia, Najib; Knoth, Oswald; Moreau, Baptiste; Wilbrandt, Ulrich; Willink, Dilya; Amon, Thomas; John, Volker
    Two transient open source solvers, OpenFOAM and ParMooN, and the commercial solver Ansys Fluent are assessed with respect to the simulation of the turbulent air flow inside and around a dairy barn. For this purpose, data were obtained in an experimental campaign at a 1:100 scaled wind tunnel model. All solvers used different meshes, discretization schemes, and turbulence models. The experimental data and numerical results agree well for time-averaged stream-wise and vertical-wise velocities. In particular, the air exchange was predicted with high accuracy by both open source solvers with relative differences less than 4% and by the commercial solver with a relative difference of 9% compared to the experimental results. With respect to the turbulent quantities, good agreements at the second (downwind) half of the barn inside and especially outside the barn could be achieved, where all codes accurately predicted the flow separation and, in many cases, the root-mean-square velocities. Deviations between simulations and experimental results regarding turbulent quantities could be observed in the first part of the barn. These deviations can be attributed to the utilization of roughness elements between inlet and barn in the experiment that were not modeled in the numerical simulations. Both open source solvers proved to be promising tools for the accurate prediction of time-dependent phenomena in an agricultural context, e.g., like the transport of particulate matter or pathogen-laden aerosols in and around agricultural buildings. © 2020 The Authors