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- ItemRaman-spectroscopy based cell identification on a microhole array chip(Basel : MDPI AG, 2014) Neugebauer, U.; Kurz, C.; Bocklitz, T.; Berger, T.; Velten, T.; Clement, J.H.; Krafft, C.; Popp, J.Circulating tumor cells (CTCs) from blood of cancer patients are valuable prognostic markers and enable monitoring responses to therapy. The extremely low number of CTCs makes their isolation and characterization a major technological challenge. For label-free cell identification a novel combination of Raman spectroscopy with a microhole array platform is described that is expected to support high-throughput and multiplex analyses. Raman spectra were registered from regularly arranged cells on the chip with low background noise from the silicon nitride chip membrane. A classification model was trained to distinguish leukocytes from myeloblasts (OCI-AML3) and breast cancer cells (MCF-7 and BT-20). The model was validated by Raman spectra of a mixed cell population. The high spectral quality, low destructivity and high classification accuracy suggests that this approach is promising for Raman activated cell sorting.
- ItemStudy of high temperature and high density plasmoids in axially symmetrical magnetic fields : final report on the joint research project(Dresden : Forschungszentrum Dresden-Rossendorf, 2009) Anikeev, A.V.; Bagryansky, P.A.; Prikhodko, V.V.; Soldatkina, E.I.; Tsidulko, Yu.A.; Kolesnikov, E.Yu.; Lizunov, A.A.; Noack, K.; Konheiser, J.; Berger, T.[no abstract available]