5 results
Search Results
Now showing 1 - 5 of 5
- ItemDistinction of nucleobases - A tip-enhanced Raman approach(Frankfurt, M. : Beilstein-Institut zur Förderung der Chemischen Wissenschaften, 2011) Treffer, R.; Lin, X.; Bailo, E.; Deckert-Gaudig, T.; Deckert, V.The development of novel DNA sequencing methods is one of the ongoing challenges in various fields of research seeking to address the demand for sequence information. However, many of these techniques rely on some kind of labeling or amplification steps. Here we investigate the intrinsic properties of tip-enhanced Raman scattering (TERS) towards the development of a novel, label-free, direct sequencing method. It is known that TERS allows the acquisition of spectral information with high lateral resolution and single-molecule sensitivity. In the presented experiments, single stranded adenine and uracil homopolymers were immobilized on different kinds of substrates (mica and gold nanoplates) and TERS experiments were conducted, which demonstrated the reproducibility of the technique. To elucidate the signal contributions from the specific nucleobases, TERS spectra were collected on single stranded calf thymus DNA with arbitrary sequence. The results show that, while the Raman signals with respect to the four nucleobases differ remarkably, specific markers can be determined for each respective base. The combination of sensitivity and reproducibility shows that the crucial demands for a sequencing procedure are met.
- ItemHorizontally resolved structures of radar backscatter from polar mesospheric layers(Göttingen : Copernicus, 2012) Latteck, R.; Singer, W.; Rapp, M.; Renkwitz, T.; Stober, G.The Leibniz-Institute of Atmospheric Physics in Kühlungsborn, Germany (IAP) installed a new powerful VHF radar on the North-Norwegian island Andøya (69.30 N, 16.04 E) from 2009 to 2011. The new Middle Atmosphere Alomar Radar System (MAARSY) replaces the existing ALWIN radar which has been in continuous operation on Andøya for more than 10 yr. MAARSY is a monostatic radar operated at 53.5 MHz with an active phased array antenna consisting of 433 Yagi antennas each connected to its own transceiver with independent control of frequency, phase and power of the transmitted signal. This arrangement provides a very high flexibility of beam forming and beam steering. It allows classical beam swinging operation as well as experiments with simultaneous multiple beams and the use of modern interferometric applications for improved studies of the Arctic atmosphere from the troposphere up to the lower thermosphere with high spatial-temporal resolution. The installation of the antenna was completed in August 2009. An initial expansion stage of 196 transceiver modules was installed in spring 2010, upgraded to 343 transceiver modules in December 2010 and the installation of the radar was completed in spring 2011. Beside standard observations of tropospheric winds and Polar Mesosphere Summer Echoes, multi-beam experiments using up to 91 beams quasi-simultaneously in the mesosphere have been carried out using the different expansion stages of the system during campaigns in 2010 and 2011. These results provided a first insight into the horizontal variability of Polar Mesosphere Summer and Winter Echoes in an area of about 80 km by 80 km with time resolutions between 3 and 9 min.
- ItemNew experiments to validate the radiation pattern of the Middle Atmosphere Alomar Radar System (MAARSY)(Göttingen : Copernicus, 2013) Renkwitz, T.; Stober, G.; Latteck, R.; Singer, W.; Rapp, M.The Middle Atmosphere Alomar Radar System (MAARSY) is a monostatic radar with an active phased array antenna designed for studies of phenomena in the mesosphere and lower thermosphere. Its design in particular the flexible beam forming and steering capability makes it to a powerful instrument to perform observations with high angular and temporal resolution. The knowledge of the actual radiation pattern is crucial to configure and analyze experiments carried out with the radar. The simulated radiation pattern is evaluated by the observation of cosmic radio emissions which are compared to a Global Sky temperature Maps model consisting of the most recent, thorough and accurate radio astronomy surveys. Additionally to these passive receive-only experiments active two-way experiments are presented, which corroborate the findings of the passive experiments.
- ItemDynamical studies on the generation of periodic surface structures by femtosecond laser pulses(Les Ulis : EDP Sciences, 2013) Rosenfeld, A.; Höhm, S.; Bonse, J.; Krüger, J.The dynamics of the formation of laser-induced periodic surface structures (LIPSS) on fused silica upon irradiation with linearly polarized fs-laser pulses (50 fs pulse duration, 800 nm center wavelength) is studied experimentally using a double pulse experiment with cross polarized pulse sequences and a trans illumination femtosecond time-resolved (0.1 ps - 1 ns) pump-probe diffraction approach. The results in both experiments confirm the importance of the ultrafast energy deposition and the laser-induced free-electron plasma in the conduction band of the solids for the formation of LIPSS.
- ItemCytoskeletal transition in patterned cells correlates with interfacial energy model(London [u.a.] : Royal Society of Chemistry, 2014) Müller, A.; Meyer, J.; Paumer, T.; Pompe, T.A cell's morphology is intricately regulated by microenvironmental cues and intracellular feedback signals. Besides biochemical factors, cell fate can be influenced by the mechanics and geometry of the surrounding matrix. The latter point was addressed herein, by studying cell adhesion on two-dimensional micropatterns. Endothelial cells were grown on maleic acid copolymer surfaces structured with stripes of fibronectin by microcontact printing. Experiments showed a biphasic behaviour of actin stress fibre spacing in dependence on the stripe width with a critical size of approx. 15 μm. In a concurrent modelling effort, cells on stripes were simulated as droplet-like structures, including variations of interfacial energy, total volume and dimensions of the nucleus. A biphasic behaviour with regard to cell morphology and area was found, triggered by the minimum of interfacial energy, with the phase transition occurring at a critical stripe width close to the critical stripe width found in the cell experiment. The correlation of experiment and simulation suggests a possible mechanism of the cytoskeletal rearrangements based on interfacial energy arguments.