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Author: Carazo-Salas, R.E. × Publisher: Washington, DC : American Chemical Society × Subject: chemistry × Subject: human ×

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    Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies
    (Washington, DC : American Chemical Society, 2014) Xi, W.; Schmidt, C.K.; Sanchez, S.; Gracias, D.H.; Carazo-Salas, R.E.; Jackson, S.P.; Schmidt, O.G.
    We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.