Search Results
Surface enhanced Raman spectroscopy-based evaluation of the membrane protein composition of the organohalide-respiring Sulfurospirillum multivorans
2021, Cialla-May, Dana, Gadkari, Jennifer, Winterfeld, Andreea, Hübner, Uwe, Weber, Karina, Diekert, Gabriele, Schubert, Torsten, Goris, Tobias, Popp, Jürgen
Bacteria often employ different respiratory chains that comprise membrane proteins equipped with various cofactors. Monitoring the protein inventory that is present in the cells under a given cultivation condition is often difficult and time-consuming. One example of a metabolically versatile bacterium is the microaerophilic organohalide-respiring Sulfurospirillum multivorans. Here, we used surface enhanced Raman spectroscopy (SERS) to quickly identify the cofactors involved in the respiration of S. multivorans. We cultured the organism with either tetrachloroethene (perchloroethylene, PCE), fumarate, nitrate, or oxygen as electron acceptors. Because the corresponding terminal reductases of the four different respiratory chains harbor different cofactors, specific fingerprint signals in SERS were expected. Silver nanostructures fabricated by means of electron beam lithography were coated with the membrane fractions extracted from the four S. multivorans cultivations, and SERS spectra were recorded. In the case of S. multivorans cultivated with PCE, the recorded SERS spectra were dominated by Raman peaks specific for Vitamin B12. This is attributed to the high abundance of the PCE reductive dehalogenase (PceA), the key enzyme in PCE respiration. After cultivation with oxygen, fumarate, or nitrate, no Raman spectral features of B12 were found. © 2020 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd
Using Raman spectroscopy in infection research
2022, Cialla-May, Dana, Rösch, Petra, Popp, Jürgen
Raman spectroscopy allows to analyze bacteria and other microorganisms label and destruction free. With different Raman techniques either colonies on agar plates or small structures like single bacterial cells can be analyzed allowing for their identification as well as enabling 2D and 3D information of intracellular bacteria or biofilms. Using surface enhanced Raman spectroscopy (SERS) allows detecting and identifying viruses as well as antibiotics relevant in the treatment of infections.