2020, Langer, Judith, de Aberasturi, Dorleta Jimenez, Aizpurua, Javier, Alvarez-Puebla, Ramon A., Auguié, Baptiste, Baumberg, Jeremy J., Bazan, Guillermo C., Bell, Steven E.J., Boisen, Anja, Brolo, Alexandre G., Choo, Jaebum, Cialla-May, Dana, Deckert, Volker, Fabris, Laura, Faulds, Karen, de Abajo, F. Javier García, Goodacre, Royston, Graham, Duncan, Haes, Amanda J., Haynes, Christy L., Huck, Christian, Itoh, Tamitake, Käll, Mikael, Kneipp, Janina, Kotov, Nicholas A., Kuang, Hua, Le Ru, Eric C., Lee, Hiang Kwee, Li, Jian-Feng, Ling, Xing Yi, Maier, Stefan A., Mayerhöfer, Thomas, Moskovits, Martin, Murakoshi, Kei, Nam, Jwa-Min, Nie, Shuming, Ozaki, Yukihiro, Pastoriza-Santos, Isabel, Perez-Juste, Jorge, Popp, Juergen, Pucci, Annemarie, Reich, Stephanie, Ren, Bin, Schatz, George C., Shegai, Timur, Schlücker, Sebastian, Tay, Li-Lin, Thomas, K. George, Tian, Zhong-Qun, Van Duyne, Richard P., Vo-Dinh, Tuan, Wang, Yue, Willets, Katherine A., Xu, Chuanlai, Xu, Hongxing, Xu, Yikai, Yamamoto, Yuko S., Zhao, Bing, Liz-Marzán, Luis M.

The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article.

2019, Alhogail, Sahar, Suaifan, Ghadeer A.R.Y, Bikker, Floris J., Kaman, Wendy E., Weber, Karina, Cialla-May, Dana, Popp, Jürgen, Zourob, Mohammed M.

A rapid, sensitive, and specific colorimetric biosensor based on the use of magnetic nanoparticles (MNPs) was designed for the detection of Pseudomonas aeruginosa in clinical samples. The biosensing platform was based on the measurement of P. aeruginosa proteolytic activity using a specific protease substrate. At the N-terminus, this substrate was covalently bound to MNPs and was linked to a gold sensor surface via cystine at the C-terminus of the substrates. The golden sensor appears black to naked eyes because of the coverage of the MNPs. However, upon proteolysis, the cleaved peptide–MNP moieties will be attracted by an external magnet, revealing the golden color of the sensor surface, which can be observed by the naked eye. In vitro, the biosensor was able to detect specifically and quantitatively the presence of P. aeruginosa with a detection limit of 102 cfu/mL in less than 1 min. The colorimetric biosensor was used to test its ability to detect in situ P. aeruginosa in clinical isolates from patients. This biochip is anticipated to be useful as a rapid point-of-care device for the diagnosis of P. aeruginosa-related infections.