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Author: Eggeling, Christian × End date: 2024 × Start date: 2020 × DDC: 570 × Has files: Yes × Publisher: Basel : MDPI ×

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    Comparison of Multiscale Imaging Methods for Brain Research
    (Basel : MDPI, 2020) Tröger, Jessica; Hoischen, Christian; Perner, Birgit; Monajembashi, Shamci; Barbotin, Aurélien; Löschberger, Anna; Eggeling, Christian; Kessels, Michael M.; Qualmann, Britta; Hemmerich, Peter
    A major challenge in neuroscience is how to study structural alterations in the brain. Even small changes in synaptic composition could have severe outcomes for body functions. Many neuropathological diseases are attributable to disorganization of particular synaptic proteins. Yet, to detect and comprehensively describe and evaluate such often rather subtle deviations from the normal physiological status in a detailed and quantitative manner is very challenging. Here, we have compared side-by-side several commercially available light microscopes for their suitability in visualizing synaptic components in larger parts of the brain at low resolution, at extended resolution as well as at super-resolution. Microscopic technologies included stereo, widefield, deconvolution, confocal, and super-resolution set-ups. We also analyzed the impact of adaptive optics, a motorized objective correction collar and CUDA graphics card technology on imaging quality and acquisition speed. Our observations evaluate a basic set of techniques, which allow for multi-color brain imaging from centimeter to nanometer scales. The comparative multi-modal strategy we established can be used as a guide for researchers to select the most appropriate light microscopy method in addressing specific questions in brain research, and we also give insights into recent developments such as optical aberration corrections.
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    Creating supported plasma membrane bilayers using acoustic pressure
    (Basel : MDPI, 2020) Sezgin, Erdinc; Carugo, Dario; Levental, Ilya; Stride, Eleanor; Eggeling, Christian
    Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.