Search Results
Thermo-Responsive Ultrafiltration Block Copolymer Membranes Based on Polystyrene-block-poly(diethyl acrylamide)
2023, Frieß, Florian V., Hartmann, Frank, Gemmer, Lea, Pieschel, Jens, Niebuur, Bart‐Jan, Faust, Matthias, Kraus, Tobias, Presser, Volker, Gallei, Markus
Within the present work, a thermo-responsive ultrafiltration membrane is manufactured based on a polystyrene-block-poly(diethyl acrylamide) block copolymer (BCP). The poly(diethyl acrylamide) block segment features a lower critical solution temperature (LCST) in water, similar to the well-known poly(N-isopropylacrylamide), but having increased biocompatibility and without exhibiting a hysteresis of the thermally induced switching behavior. The BCP is synthesized via sequential “living” anionic polymerization protocols and analyzed by 1H-NMR spectroscopy, size exclusion chromatography, and differential scanning calorimetry. The resulting morphology in the bulk state is investigated by transmission electron microscopy (TEM) and small-angle X-ray scattering (SAXS) revealing the intended hexagonal cylindrical morphology. The BCPs form micelles in a binary mixture of tetrahydrofuran and dimethylformamide, where BCP composition and solvent affinities are discussed in light of the expected structure of these micelles and the resulting BCP membrane formation. The membranes are manufactured using the non-solvent induced phase separation (NIPS) process and are characterized via scanning electron microscopy (SEM) and water permeation measurements. The latter are carried out at room temperature and at 50 °C revealing up to a 23-fold increase of the permeance, when crossing the LCST of the poly(diethyl acrylamide) block segment in water.
A new Lagrangian in-time particle simulation module (Itpas v1) for atmospheric particle dispersion
2021, Faust, Matthias, Wolke, Ralf, Münch, Steffen, Funk, Roger, Schepanski, Kerstin
Trajectory models are intuitive tools for airflow studies. But in general, they are limited to non-turbulent, i.e. laminar flow, conditions. Therefore, trajectory models are not particularly suitable for investigating airflow within the turbulent atmospheric boundary layer. To overcome this, a common approach is handling the turbulent uncertainty as a random deviation from a mean path in order to create a statistic of possible solutions which envelops the mean path. This is well known as the Lagrangian particle dispersion model (LPDM). However, the decisive factor is the representation of turbulence in the model, for which widely used models such as FLEXPART and HYSPLIT use an approximation. A conceivable improvement could be the use of a turbulence parameterisation approach based on the turbulent kinetic energy (TKE) at high temporal resolution. Here, we elaborated this approach and developed the LPDM Itpas, which is coupled online to the German Weather Service's mesoscale weather forecast model COSMO. It benefits from the prognostically calculated TKE as well as from the high-frequency wind information. We demonstrate the model's applicability for a case study on agricultural particle emission in eastern Germany. The results obtained are discussed with regard to the model's ability to describe particle transport within a turbulent boundary layer. Ultimately, the simulations performed suggest that the newly introduced method based on prognostic TKE sufficiently represents the particle transport.
Airborne bacterial emission fluxes from manure-fertilized agricultural soil
2020, Thiel, Nadine, Münch, Steffen, Behrens, Wiebke, Junker, Vera, Faust, Matthias, Biniasch, Oliver, Kabelitz, Tina, Siller, Paul, Boedeker, Christian, Schumann, Peter, Roesler, Uwe, Amon, Thomas, Schepanski, Kerstin, Funk, Roger, Nübel, Ulrich
This is the first study to quantify the dependence on wind velocity of airborne bacterial emission fluxes from soil. It demonstrates that manure bacteria get aerosolized from fertilized soil more easily than soil bacteria, and it applies bacterial genomic sequencing for the first time to trace environmental faecal contamination back to its source in the chicken barn. We report quantitative, airborne emission fluxes of bacteria during and following the fertilization of agricultural soil with manure from broiler chickens. During the fertilization process, the concentration of airborne bacteria culturable on blood agar medium increased more than 600 000-fold, and 1 m3 of air carried 2.9 × 105 viable enterococci, i.e. indicators of faecal contamination which had been undetectable in background air samples. Trajectory modelling suggested that atmospheric residence times and dispersion pathways were dependent on the time of day at which fertilization was performed. Measurements in a wind tunnel indicated that airborne bacterial emission fluxes from freshly fertilized soil under local climatic conditions on average were 100-fold higher than a previous estimate of average emissions from land. Faecal bacteria collected from soil and dust up to seven weeks after fertilization could be traced to their origins in the poultry barn by genomic sequencing. Comparative analyses of 16S rRNA gene sequences from manure, soil and dust showed that manure bacteria got aerosolized preferably, likely due to their attachment to low-density manure particles. Our data show that fertilization with manure may cause substantial increases of bacterial emissions from agricultural land. After mechanical incorporation of manure into soil, however, the associated risk of airborne infection is low.
Agricultural fertilization with poultry manure results in persistent environmental contamination with the pathogen Clostridioides difficile
2021, Frentrup, Martinique, Thiel, Nadine, Junker, Vera, Behrens, Wiebke, Münch, Steffen, Siller, Paul, Kabelitz, Tina, Faust, Matthias, Indra, Alexander, Baumgartner, Stefanie, Schepanski, Kerstin, Amon, Thomas, Roesler, Uwe, Funk, Roger, Nübel, Ulrich
During a field experiment applying broiler manure for fertilization of agricultural land, we detected viable Clostridioides (also known as Clostridium) difficile in broiler faeces, manure, dust and fertilized soil. A large diversity of toxigenic C. difficile isolates was recovered, including PCR ribotypes common from human disease. Genomic relatedness of C. difficile isolates from dust and from soil, recovered more than 2 years after fertilization, traced their origins to the specific chicken farm that had delivered the manure. We present evidence of long-term contamination of agricultural soil with manure-derived C. difficile and demonstrate the potential for airborne dispersal of C. difficile through dust emissions during manure application. Clostridioides genome sequences virtually identical to those from manure had been recovered from chicken meat and from human infections in previous studies, suggesting broiler-associated C. difficile are capable of zoonotic transmission.