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Author: Hassa, Julia × End date: 2024 × Start date: 2020 × DDC: 570 × Has files: Yes × Publisher: Basel : MDPI AG ×

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    The role of petrimonas mucosa ING2-E5at in mesophilic biogas reactor systems as deduced from multiomics analyses
    (Basel : MDPI AG, 2020) Maus, Irena; Tubbesing, Tom; Wibberg, Daniel; Heyer, Robert; Hassa, Julia; Tomazetto, Geizecler; Huang, Liren; Bunk, Boyke; Spröer, Cathrin; Benndorf, Dirk; Zverlov, Vladimir; Pühler, Alfred; Klocke, Michael; Sczyrba, Alexander; Schlüter, Andreas
    Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Microbiome diversity and community-level change points within manure-based small biogas plants
    (Basel : MDPI AG, 2020) Theuerl, Susanne; Klang, Johanna; Hülsemann, Benedikt; Mächtig, Torsten; Hassa, Julia
    Efforts to integrate biogas plants into bioeconomy concepts will lead to an expansion of manure-based (small) biogas plants, while their operation is challenging due to critical characteristics of some types of livestock manure. For a better process understanding, in this study, three manure-based small biogas plants were investigated with emphasis on microbiome diversity. Due to varying digester types, feedstocks, and process conditions, 16S rRNA gene amplicon sequencing showed differences in the taxonomic composition. Dynamic variations of each investigated biogas plant microbiome over time were analyzed by terminal restriction fragment length polymorphism (TRFLP), whereby nonmetric multidimensional scaling (NMDS) revealed two well-running systems, one of them with a high share of chicken manure, and one unstable system. By using Threshold Indicator Taxa Analysis (TITAN), community-level change points at ammonium and ammonia concentrations of 2.25 g L-1 and 193 mg L-1 or volatile fatty acid concentrations of 0.75 g L-1were reliably identified which are lower than the commonly reported thresholds for critical process stages based on chemical parameters. Although a change in the microbiome structure does not necessarily indicate an upcoming critical process stage, the recorded community-level change points might be a first indication to carefully observe the process. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.