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- ItemAufbau einer Ex-situ-Sammlung von methanbildenden Archaea aus Biogasanlagen im ländlichen Raum (Methanogenic Archaea Culture Collection, MACC) : Schlussbericht zum Projekt ; Schlussbericht im Rahmen des Förderprogramms "Nachwachsende Rohstoffe" ; Berichtszeitraum: 01.09.2010 bis 31.08.2012(Hannover : Technische Informationsbibliothek, 2012) Klocke, Michael; Hahnke, Sarah[no abstract available]
- ItemMonitoring der methanbildenden Mikroflora in Praxis-Biogasanlagen im ländlichen Raum : Analyse des Ist-Zustandes und Entwicklung eines quantitativen Nachweissystems(Hannover : Technische Informationsbibliothek, 2009) Klocke, Michael; Nettmann, Edith; Bergmann, IngoDie Produktion von Biogas aus landwirtschaftlichen Primärprodukten oder Reststoffen stellt einen wesentlichen Beitrag zur Reduktion des Co2-Ausstoßes sowie zur Entwicklung einer nachhaltigen Landbewirtschaftung dar. Im Rahmen dieses Projektes soll daher die Artenzusammensetzung der methanogenen Mikroflora in ausgewählten Praxis-Biogasanlagen anhand ihrer 16S rDNA analysisert werden.
- ItemDevelopment of a flow-fluorescence in situhybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor(London : BioMed Central, 2013) Nettmann, Edith; Fröhling, Antje; Heeg, Kathrin; Klocke, Michael; Schlüter, Oliver; Mumme, JanBackground: The production of bio-methane from renewable raw material is of high interest because of the increasing scarcity of fossil fuels. The process of biomethanation is based on the inter- and intraspecific metabolic activity of a highly diverse and dynamic microbial community. The community structure of the microbial biocenosis varies between different biogas reactors and the knowledge about these microbial communities is still fragmentary. However, up to now no approaches are available allowing a fast and reliable access to the microbial community structure. Hence, the aim of this study was to originate a Flow-FISH protocol, namely a combination of flow cytometry and fluorescence in situ hybridization, for the analysis of the metabolically active microorganisms in biogas reactor samples. With respect to the heterogenic texture of biogas reactor samples and to collect all cells including those of cell aggregates and biofilms the development of a preceding purification procedure was indispensable. Results: Six different purification procedures with in total 29 modifications were tested. The optimized purification procedure combines the use of the detergent sodium hexametaphosphate with ultrasonic treatment and a final filtration step. By this treatment, the detachment of microbial cells from particles as well as the disbandment of cell aggregates was obtained at minimized cell loss. A Flow-FISH protocol was developed avoiding dehydration and minimizing centrifugation steps. In the exemplary application of this protocol on pure cultures as well as biogas reactor samples high hybridization rates were achieved for commonly established domain specific oligonucleotide probes enabling the specific detection of metabolically active bacteria and archaea. Cross hybridization and autofluorescence effects could be excluded by the use of a nonsense probe and negative controls, respectively. Conclusions: The approach described in this study enables for the first time the analysis of the metabolically active fraction of the microbial communities within biogas reactors by Flow-FISH.
- ItemCharakterisierung der mikrobiellen Diversität in Biogasreaktoren bei semikontinuierlicher Beschickung mit pflanzlicher Biomasse und Gülle in unterschiedlichen Belastungsstufen : Schlussbericht zum Projekt(Hannover : Technische Informationsbibliothek (TIB), 2009) Klocke, Michael; Sontag, Mario; Bergmann, Ingo; Mähnert, Pia[no abstract available]
- ItemGrundlagen der Biogasgewinnung aus pflanzlicher Biomasse: Systemanalyse der mikrobiologischen Stoffumwandlung unter besonderer Berücksichtigung des Einflusses der Substratbereitstellung, der Intermediatbildung sowie der Prozessführung, Teilprojekt 03SF0317M: Erfassung der mikrobiellen Biozönosen in Monovergärungen von Getreide-Ganzpflanzensilage in mehrstufigen Fermentationen (bioleaching-Verfahren) : BCN - Biogas-Crops-Network(Hannover : Technische Informationsbibliothek, 2009) Klocke, Michael[no abstract available]
- ItemVerbundvorhaben Bioraffinerie-Modul zum gerichtet-fermentativen Aufschluss von Biomasse für eine kombinierte energetische und stoffliche Verwertung - FABES-Modul, Teilprojekt 4: Mikrobiologische Prozessanalyse ; Forschungsvorhaben im Rahmen des BMBF-Förderprogramms BioEnergie 2021 - Forschung für die Nutzung von Biomasse ; Laufzeit des Vorhabens/Berichtszeitraum: 01.04.2009 bis 31.03.2012(Hannover : Technische Informationsbibliothek, 2013) Klocke, Michael; Hanreich, Angelika[no abstract available]
- ItemDynamic variation of the microbial community structure during the long-time mono-fermentation of maize and sugar beet silage(Milton Park : Taylor & Francis, 2015) Klang, Johanna; Theuerl, Susanne; Szewzyk, Ulrich; Huth, Markus; Tölle, Rainer; Klocke, MichaelThis study investigated the development of the microbial community during a long-term (337 days) anaerobic digestion of maize and sugar beet silage, two feedstocks that significantly differ in their chemical composition. For the characterization of the microbial dynamics, the community profiling method terminal restriction fragment length polymorphism (TRFLP) in combination with a cloning-sequencing approach was applied. Our results revealed a specific adaptation of the microbial community to the supplied feedstocks. Based on the high amount of complex compounds, the anaerobic conversion rate of maize silage was slightly lower compared with the sugar beet silage. It was demonstrated that members from the phylum Bacteroidetes are mainly involved in the degradation of low molecular weight substances such as sugar, ethanol and acetate, the main compounds of the sugar beet silage. It was further shown that species of the genus Methanosaeta are highly sensitive against sudden stress situations such as a strong decrease in the ammonium nitrogen (NH4 +-N) concentration or a drop of the pH value. In both cases, a functional compensation by members of the genera Methanoculleus and/or Methanosarcina was detected. However, the overall biomass conversion of both feedstocks proceeded efficiently as a steady state between acid production and consumption was recorded, which further resulted in an equal biogas yield.
- ItemImpact of surface structure and feed gas composition on Bacillus subtilis endospore inactivation during direct plasma treatment(Lausanne : Frontiers Media, 2015) Hertwig, Christian; Steins, Veronika; Reineke, Kai; Rademacher, Antje; Klocke, Michael; Rauh, Cornelia; Schlüter, OliverThis study investigated the inactivation efficiency of cold atmospheric pressure plasma treatment on Bacillus subtilis endospores dependent on the used feed gas composition and on the surface, the endospores were attached on. Glass petri-dishes, glass beads, and peppercorns were inoculated with the same endospore density and treated with a radio frequency plasma jet. Generated reactive species were detected using optical emission spectroscopy. A quantitative polymerase chain reaction (qPCR) based ratio detection system was established to monitor the DNA damage during the plasma treatment. Argon + 0.135% vol. oxygen + 0.2% vol. nitrogen as feed gas emitted the highest amounts of UV-C photons and considerable amount of reactive oxygen and nitrogen species. Plasma generated with argon + 0.135% vol. oxygen was characterized by the highest emission of reactive oxygen species (ROS), whereas the UV-C emission was negligible. The use of pure argon showed a negligible emission of UV photons and atomic oxygen, however, the emission of vacuum (V)UV photons was assumed. Similar maximum inactivation results were achieved for the three feed gas compositions. The surface structure had a significant impact on the inactivation efficiency of the plasma treatment. The maximum inactivation achieved was between 2.4 and 2.8 log10 on glass petri-dishes and 3.9 to 4.6 log10 on glass beads. The treatment of peppercorns resulted in an inactivation lower than 1.0 log10. qPCR results showed a significant DNA damage for all gas compositions. Pure argon showed the highest results for the DNA damage ratio values, followed by argon + 0.135% vol. oxygen + 0.2% vol. nitrogen. In case of argon + 0.135% vol. oxygen the inactivation seems to be dominated by the action of ROS. These findings indicate the significant role of VUV and UV photons in the inactivation process of B. subtilis endospores.
- ItemBiofilme in Biogasanlagen : Struktur, Einfluss auf die Biogasausbeute und Optimierung technischer Systeme zur Rückhaltung der mikrobiellen Biomasse : Schlussbericht zum Forschungsbverbund BIOGAS-BIOFILM(Hannover : Technische Informationsbibliothek, 2015) Bergmann, Ingo; Klocke, Michael[no abstract available]
- ItemEtablierung eines core-Mikrobioms für Biogasanlagen : Genom-Sequenzierung von Isolaten aus Biogasanlagen und Mapping von Metagenom-Datensätzen : Schlussbericht zum Forschungsverbund Biogas-Core(Hannover : Technische Informationsbibliothek, 2017) Klocke, Michael[no abstract available]