Filters

20152

More filters

2019 - 201912020 - 20221
Reset filters

Settings

Author: Koch, Britta × Author: Sanchez, Samuel × Version: publishedVersion × WGL Institute: IFWD ×

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    A size dependent evaluation of the cytotoxicity and uptake of nanographene oxide
    (London [u.a.] : RSC, 2015) Mendes, Rafael Gregorio; Koch, Britta; Bachmatiuk, Alicja; Ma, Xing; Sanchez, Samuel; Damm, Christine; Schmidt, Oliver G.; Gemming, Thomas; Eckert, Jürgen; Rümmeli, Mark H.
    Graphene oxide (GO) has attracted great interest due to its extraordinary potential for biomedical application. Although it is clear that the naturally occurring morphology of biological structures is crucial to their precise interactions and correct functioning, the geometrical aspects of nanoparticles are often ignored in the design of nanoparticles for biological applications. A few in vitro and in vivo studies have evaluated the cytotoxicity and biodistribution of GO, however very little is known about the influence of flake size and cytotoxicity. Herein, we aim at presenting an initial cytotoxicity evaluation of different nano-sized GO flakes for two different cell lines (HeLa (Kyoto) and macrophage (J7742)) when they are exposed to samples containing different sized nanographene oxide (NGO) flakes (mean diameter of 89 and 277 nm). The obtained data suggests that the larger NGO flakes reduce cell viability as compared to smaller flakes. In addition, the viability reduction correlates with the time and the concentration of the NGO nanoparticles to which the cells are exposed. Uptake studies were also conducted and the data suggests that both cell lines internalize the GO nanoparticles during the incubation periods studied.
  • Thumbnail Image
    Item
    Dimensionality of rolled-up nanomembranes controls neural stem cell migration mechanism
    (Washington D.C. : American Chemical Society, 2015) Koch, Britta; Meyer, Anne K.; Helbig, Linda; Harazim, Stefan M.; Storch, Alexander; Sanchez, Samuel; Schmidt, Oliver G.
    We employ glass microtube structures fabricated by rolledup nanotechnology to infer the influence of scaffold dimensionality and cell confinement on neural stem cell (NSC) migration. Thereby, we observe a pronounced morphology change that marks a reversible mesenchymal to amoeboid migration mode transition. Space restrictions preset by the diameter of nanomembrane topography modify the cell shape toward characteristics found in living tissue. We demonstrate the importance of substrate dimensionality for the migration mode of NSCs and thereby define rolled-up nanomembranes as the ultimate tool for single-cell migration studies.