2020, Pascual, Javier, Hahnke, Sarah, Abendroth, Christian, Langer, Thomas, Ramm, Patrice, Klocke, Michael, Luschnig, Olaf, Porcar, Manuel

Here, we present the genome sequence and annotation of the novel bacterial strain HV4-5-C5C, which may represent a new genus within the family Oscillospiraceae (order Eubacteriales). This strain is a potential keystone species in the hydrolysis of complex polymers during anaerobic digestion of biomass. © 2020 Pascual et al.

2023, Küchler, Jan, Willenbücher, Katharina, Reiß, Elisabeth, Nuß, Lea, Conrady, Marius, Ramm, Patrice, Schimpf, Ulrike, Reichl, Udo, Szewzyk, Ulrich, Benndorf, Dirk

The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of Lentinula edodes revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates.

2020, Hahnke, Sarah, Abendroth, Christian, Pascual, Javier, Langer, Thomas, Ramm, Patrice, Klocke, Michael, Luschnig, Olaf, Porcar, Manuel

Here, we present the genome sequence and annotation of the bacterial strain HV4-5-A1G, a potentially new Clostridium species. Based on its genomic data, this strain may act as a keystone microorganism in the hydrolysis of complex polymers, as well as in the different acidogenesis and acetogenesis steps during anaerobic digestion. © 2020 Hahnke et al.

2020, Hahnke, Sarah, Abendroth, Christian, Pascual, Javier, Langer, Thomas, Codoñer, Francisco M., Ramm, Patrice, Klocke, Michael, Luschnig, Olaf, Porcare, Manuel

Here, we present the genome sequence and annotation of HV4-6-C5C, a bacterial strain isolated from a mesophilic two-stage laboratory-scale leach bed biogas reactor system. Strain HV4-6-C5C may represent a new genus of the family Bacteroidaceae and may have a key role in acidogenesis and acetogenesis steps during anaerobic biomass digestion. © 2019 Hahnke et al.

2018, Hahnke, Sarah, Abendroth, Christian, Langer, Thomas, Codoñer, Francisco M., Ramm, Patrice, Porcar, Manuel, Luschnig, Olaf, Klocke, Michael

A new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratoryscale leach bed biogas system. The draft annotated genome sequence presented in this study and 16S rRNA gene sequence analysis indicated the affiliation of HV4-5- B5C with the family Ruminococcaceae outside recently described genera. © 2018 Hahnke et al.

2010, Linke, Bernd, Mumme, Jan, Ramm, Patrice

[no abstract available]