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Author: Reuter, Cornelia ×

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    2-LED-μspectrophotometer for rapid on-site detection of pathogens using noble-metal nanoparticle-based colorimetric assays
    (Basel : MDPI, 2020) Reuter, Cornelia; Urban, Matthias; Arnold, Manuel; Stranik, Ondrej; Csáki, Andrea; Fritzsche, Wolfgang
    Novel point-of-care compatible methods such as colorimetric assays have become increasingly important in the field of early pathogen detection. A simple and hand-held prototype device for carrying out DNA-amplification assay based on plasmonic nanoparticles in the colorimetric detection is presented. The low-cost device with two channels (sample and reference) consists of two spectrally different light emitting diodes (LEDs) for detection of the plasmon shift. The color change of the gold-nanoparticle-DNA conjugates caused by a salt-induced aggregation test is examined in particular. A specific and sensitive detection of the waterborne human pathogen Legionella pneumophila is demonstrated. This colorimetric assay, with a simple assay design and simple readout device requirements, can be monitored in real-time on-site. © 2020 by the authors.
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    Chip-based duplex real-time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.
    (Oxford [u.a.] : Wiley-Blackwell, 2020) Reuter, Cornelia; Hentschel, Stefanie; Breitenstein, Antje; Heinrich, Eileen; Aehlig, Oliver; Henkel, Thomas; Csáki, Andrea; Fritzsche, Wolfgang
    Based on biomolecular methods, rapid and selective identification of human pathogenic water organisms becomes an important issue. Legionella spp., are pathogenic water bacteria with worldwide significance. Prevalent detection methods for these microorganisms are time and/or cost intensive. We describe a detection setup and relating DNA assay. A miniaturized real-time polymerase chain reaction (real-time PCR) for direct on-line discrimination of Legionella pneumophila and Legionella spp. was established and integrated into a real-time PCR-chip-system. The PCR-chip device combines a temperature controlling unit and a fluorescence intensity measurement. It was designed to achieve rapid amplification, using an approach of real-time fluorescence read out with the intercalating dye EvaGreen® and melting curve analysis, without requiring multiple probes. The presented results exhibit reproducibility and good sensitivity, showing that the setup is suitable for robust, rapid and cost-efficient detection and monitoring of a variety of Legionella spp.in urban water samples.