(Washington D.C. : American Chemical Society, 2015) Koch, Britta; Meyer, Anne K.; Helbig, Linda; Harazim, Stefan M.; Storch, Alexander; Sanchez, Samuel; Schmidt, Oliver G.
We employ glass microtube structures fabricated by rolledup
nanotechnology to infer the influence of scaffold dimensionality and cell
confinement on neural stem cell (NSC) migration. Thereby, we observe a
pronounced morphology change that marks a reversible mesenchymal to
amoeboid migration mode transition. Space restrictions preset by the
diameter of nanomembrane topography modify the cell shape toward
characteristics found in living tissue. We demonstrate the importance of
substrate dimensionality for the migration mode of NSCs and thereby
define rolled-up nanomembranes as the ultimate tool for single-cell
migration studies.
