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Author: Sicher, Claudia ×

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    Detecting Bacteria on Wounds with Hyperspectral Imaging in Fluorescence Mode
    (Berlin : De Gruyter, 2020) Hornberger, Christoph.; Herrmann, Bert. H.; Daeschlein, Georg; Podewils, Sebastian von; Sicher, Claudia; Kuhn, Jana; Masur, Kai; Meister, Mareike; Wahl, Philip
    Chronic non-healing wounds represent an increasing problem. In order to enable physicians and nurses to make evidence based decisions on wound treatment, the professional societies call for supporting tools to be offered to physicians. Oxygen supply, bacteria colonization and other parameters influence the healing process. So far, these parameters cannot be monitored in an objective and routinely manner. Existing methods like the microbiological analysis of wound swabs, mean a great deal of effort and partly a long delay. In this paper 42 fluorescence images from 42 patients with diabetic foot ulcer, recorded with a hyperspectral imaging system (TIVITA®), converted for fluorescence imaging, were analysed. Beside the fluorescence images, information about the bacterial colonization is available from microbiological analysis of wound swabs. After preprocessing, principal component analysis, PCA, is used for data analysis with a 405 nm excitation wavelength, the emission wavelength range 510 - 745 nm is used for analysis. After dividing the data into a training and a test dataset it could be shown, that bacteria are detectable in the wound area. A quantification in bacterial colonization counts (BCC) was not in the focus of the research in this study stage.
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    Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
    ([London] : Macmillan Publishers Limited, part of Springer Nature, 2021) Glaab, Johannes; Lobo-Ploch, Neysha; Cho, Hyun Kyong; Filler, Thomas; Gundlach, Heiko; Guttmann, Martin; Hagedorn, Sylvia; Lohan, Silke B.; Mehnke, Frank; Schleusener, Johannes; Sicher, Claudia; Sulmoni, Luca; Wernicke, Tim; Wittenbecher, Lucas; Woggon, Ulrike; Zwicker, Paula; Kramer, Axel; Meinke, Martina C.; Kneissl, Michael; Weyers, Markus; Winterwerber, Ulrike; Einfeldt, Sven
    Multiresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) cause serious postoperative infections. A skin tolerant far-UVC (< 240 nm) irradiation system for their inactivation is presented here. It uses UVC LEDs in combination with a spectral filter and provides a peak wavelength of 233 nm, with a full width at half maximum of 12 nm, and an irradiance of 44 µW/cm2. MRSA bacteria in different concentrations on blood agar plates were inactivated with irradiation doses in the range of 15–40 mJ/cm2. Porcine skin irradiated with a dose of 40 mJ/cm2 at 233 nm showed only 3.7% CPD and 2.3% 6-4PP DNA damage. Corresponding irradiation at 254 nm caused 11–14 times higher damage. Thus, the skin damage caused by the disinfectant doses is so small that it can be expected to be compensated by the skin's natural repair mechanisms. LED-based far-UVC lamps could therefore soon be used in everyday clinical practice to eradicate multiresistant pathogens directly on humans.