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- ItemKaskadennutzung von Lignocellulose : LX-Verfahren trifft auf B. coagulans(Heidelberg : Spektrum, 2020) Schroedter, Linda; Streffer, Friedrich; Streffer, Katrin; Unger, Peter; Venus, JoachimInvestigating alternatives for petrobased substrates, lignocellulose is an interesting yet complex feedstock that offers various possibilities for the design of new and sustainable chemical routes. The novel energy-saving LX-pretreatment was combined with thermophilic Bacillus coagulans. By this, corn straw was used in an innovative cascade obtaining biogas, lignin as well as polymerisable L-(+)-lactic acid of over 99 percents optical purity. © 2020, Die Autoren.
- ItemA brief dataset on the model-based evaluation of the growth performance of Bacillus coagulans and l-lactic acid production in a lignin-supplemented medium(Amsterdam : Elsevier, 2017) Glaser, Robert; Venus, JoachimThe data presented in this article are related to the research article entitled “Model-based characterization of growth performance and l-lactic acid production with high optical purity by thermophilic Bacillus coagulans in a lignin-supplemented mixed substrate medium (R. Glaser and J. Venus, 2016) [1]”. This data survey provides the information on characterization of three Bacillus coagulans strains. Information on cofermentation of lignocellulose-related sugars in lignin-containing media is given. Basic characterization data are supported by optical-density high-throughput screening and parameter adjustment to logistic growth models. Lab scale fermentation procedures are examined by model adjustment of a Monod kinetics-based growth model. Lignin consumption is analyzed using the data on decolorization of a lignin-supplemented minimal medium.
- ItemLimited life cycle andcost assessment for the bioconversion of lignin‐derived aromatics into adipic acid(New York, NY [u.a.] : Wiley, 2020) van Duuren, Jozef B.J.H.; de Wild, Paul J.; Starck, Sören; Bradtmöller, Christian; Selzer, Mirjam; Mehlmann, Kerstin; Schneider, Roland; Kohlstedt, Michael; Poblete‐Castr, Ignacio; Stolzenberger, Jessica; Barton, Nadja; Fritz, Michel; Scholl, Stephan; Venus, Joachim; Wittmann, ChristophLignin is an abundant and heterogeneous waste byproduct of the cellulosic industry, which has the potential of being transformed into valuable biochemicals via microbial fermentation. In this study, we applied a fast-pyrolysis process using softwood lignin resulting in a two-phase bio-oil containing monomeric and oligomeric aromatics without syringol. We demonstrated that an additional hydrodeoxygenation step within the process leads to an enhanced thermochemical conversion of guaiacol into catechol and phenol. After steam bath distillation, Pseudomonas putida KT2440-BN6 achieved a percent yield of cis, cis-muconic acid of up to 95 mol% from catechol derived from the aqueous phase. We next established a downstream process for purifying cis, cis-muconic acid (39.9 g/L) produced in a 42.5 L fermenter using glucose and benzoate as carbon substrates. On the basis of the obtained values for each unit operation of the empirical processes, we next performed a limited life cycle and cost analysis of an integrated biotechnological and chemical process for producing adipic acid and then compared it with the conventional petrochemical route. The simulated scenarios estimate that by attaining a mixture of catechol, phenol, cresol, and guaiacol (1:0.34:0.18:0, mol ratio), a titer of 62.5 (g/L) cis, cis-muconic acid in the bioreactor, and a controlled cooling of pyrolysis gases to concentrate monomeric aromatics in the aqueous phase, the bio-based route results in a reduction of CO2-eq emission by 58% and energy demand by 23% with a contribution margin for the aqueous phase of up to 88.05 euro/ton. We conclude that the bio-based production of adipic acid from softwood lignins brings environmental benefits over the petrochemical procedure and is cost-effective at an industrial scale. Further research is essential to achieve the proposed cis, cis-muconic acid yield from true lignin-derived aromatics using whole-cell biocatalysts. © 2020 Wiley Periodicals, Inc.
- ItemAssessing the organic fraction of municipal solid wastes for the production of lactic acid(Amsterdam [u.a.] : Elsevier, 2019) López-Gómez, J. Pablo; Latorre-Sánchez, Marcos; Unger, Peter; Schneider, Roland; Coll Lozano, Caterina; Venus, JoachimWith an estimated yearly production of about 140 Mt in the EU, conventionally, the organic fraction of municipal solid wastes (OFMSW) has been disposed in landfills with negative environmental effects. Nonetheless, the chemical composition of this residue make it a substrate with great bioconversion potential. In this study, OFMSW from Spanish municipal treatment plants, were evaluated for the production of LA. Samples were identified according to the sorting mechanisms employed for their collection in: (A) separately collected, (B) non-separately collected and (C) separately collected+paper/cardboard. Enzymatic hydrolysis was used to produce hydrolysates A, B and C accordingly. Hydrolysate A showed the highest total sugars and glucose content with values of 70 and 55 g·L−1, respectively. Following the characterisation, a screening showed that growth of B. coagulans was possible in all three hydrolysates. Furthermore, lab scale fermentations showed that LA final concentrations could reach around 60 g·L−1, with yields from total sugars of above 0.60 g·g−1. A technical scale fermentation of the hydrolysate A resulted in a final LA concentration of 60.7 g·L−1, a yield of 0.71 g·g−1 with a productivity of 2.68 g·L−1·h−1. Overall, it was estimated that 0.23 g of LA could be produced from one g of dry OFMSW.
- ItemA simple biorefinery concept to produce 2g-lactic acid from Sugar Beet Pulp (SBP): A high-value target approach to valorize awaste stream(Basel : MDPI AG, 2020) De Oliveira, Regiane Alves; Schneider, Roland; Lunelli, Betânia Hoss; Rossell, Carlos Eduardo Vaz; Filho, Rubens Maciel; Venus, JoachimLactic acid is a high-value molecule with a vast number of applications. Its production in the biorefineries model is a possibility for this sector to aggregate value to its production chain. Thus, this investigation presents a biorefinery model based on the traditional sugar beet industry proposing an approach to produce lactic acid from a waste stream. Sugar beet is used to produce sugar and ethanol, and the remaining pulp is sent to animal feed. Using Bacillus coagulans in a continuous fermentation, 2781.01 g of lactic acid was produced from 3916.91 g of sugars from hydrolyzed sugar beet pulp, with a maximum productivity of 18.06 g L-1h-1. Without interfering in the sugar production, ethanol, or lactic acid, it is also possible to produce pectin and phenolic compounds in the biorefinery. The lactic acid produced was purified by a bipolar membrane electrodialysis and the recovery reached 788.80 g/L with 98% w/w purity. © 2020 by the authors.
- ItemProduction of Lactic Acid from Carob, Banana and Sugarcane Lignocellulose Biomass(Basel : MDPI AG, 2020) Azaizeh, Hassan; Abu Tayeh, Hiba N.; Schneider, Roland; Klongklaew, Augchararat; Venus, JoachimLignocellulosic biomass from agricultural residues is a promising feedstock for lactic acid (LA) production. The aim of the current study was to investigate the production of LA from different lignocellulosic biomass. The LA production from banana peduncles using strain Bacillus coagulans with yeast extract resulted in 26.6 g LA·L-1, and yield of 0.90 g LA·g-1 sugars. The sugarcane fermentation with yeast extract resulted in 46.5 g LA·L-1, and yield of 0.88 g LA·g-1 sugars. Carob showed that addition of yeast extract resulted in higher productivity of 3.2 g LA·L-1·h-1 compared to without yeast extract where1.95 g LA·L-1·h-1 was obtained. Interestingly, similar LA production was obtained by the end where 54.8 and 51.4 g·L-1 were obtained with and without yeast extract, respectively. A pilot scale of 35 L using carob biomass fermentation without yeast extract resulted in yield of 0.84 g LA·g-1 sugars, and productivity of 2.30 g LA·L-1·h-1 which indicate a very promising process for future industrial production of LA.
- ItemBatch and continuous lactic acid fermentation based on a multi-substrate approach(Basel : MDPI AG, 2020) Olszewska-Widdrat, Agata; Alexandri, Maria; López-Gómez, José Pablo; Schneider, Roland; Venus, JoachimThe utilisation of waste materials and industrial residues became a priority within the bioeconomy concept and the production of biobased chemicals. The aim of this study was to evaluate the feasibility to continuously produce L-lactic acid from different renewable substrates, in a multi-substrate strategy mode. Based on batch experiments observations, Bacillus coagulans A534 strain was able to continuously metabolise acid whey, sugar beet molasses, sugar bread, alfalfa press green juice and tapioca starch. Additionally, reference experiments showed its behaviour in standard medium. Continuous fermentations indicated that the highest productivity was achieved when molasses was employed with a value of 10.34 g·L−1·h−1, while the lactic acid to sugar conversion yield was 0.86 g·g−1 . This study demonstrated that LA can be efficiently produced in continuous mode regardless the substrate, which is a huge advantage in comparison to other platform chemicals. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemValorising agro-industrial wastes within the circular bioeconomy concept: The case of defatted rice bran with emphasis on bioconversion strategies(Basel : MDPI AG, 2020) Alexandri, Maria; López-Gómez, José Pablo; Olszewska-Widdrat, Agata; Venus, JoachimThe numerous environmental problems caused by the extensive use of fossil resources have led to the formation of the circular bioeconomy concept. Renewable resources will constitute the cornerstone of this new, sustainable model, with biomass presenting a huge potential for the production of fuels and chemicals. In this context, waste and by-product streams from the food industry will be treated not as "wastes" but as resources. Rice production generates various by-product streams which currently are highly unexploited, leading to environmental problems especially in the countries that are the main producers. The main by-product streams include the straw, the husks, and the rice bran. Among these streams, rice bran finds applications in the food industry and cosmetics, mainly due to its high oil content. The high demand for rice bran oil generates huge amounts of defatted rice bran (DRB), the main by-product of the oil extraction process. The sustainable utilisation of this by-product has been a topic of research, either as a food additive or via its bioconversion into value-added products and chemicals. This review describes all the processes involved in the efficient bioconversion of DRB into biotechnological products. The detailed description of the production process, yields and productivities, as well as strains used for the production of bioethanol, lactic acid and biobutanol, among others, are discussed. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemOrganic fraction of municipal solid waste for the production of L-lactic acid with high optical purity(2020) López-Gómez, José Pablo; Alexandri, Maria; Schneider, Roland; Latorre-Sánchez, Marcos; Coll Lozano, Caterina; Venus, JoachimThe organic fraction of municipal solid waste (OFMSW) is an abundant biowaste with great potential in the bioeconomy model. Previous reports have demonstrated that OFMSW hydrolysates are good substrates for lactic acid (LA) production. However, LA can exist in two enantiomeric forms (L- and D-) and most commercial LA applications require a high enantiomeric purity, typically of the L-isomer. Due to natural occurring bacteria in the waste, a mixture of D- and L-LA can form in the substrate, reducing the final enantiomeric purity of the product and limiting its commercial application. In the research reported in this article, hydrolysates from OFMSW were evaluated for the production L-LA with high enantiomeric purity. Firstly, a pre-treatment with monopolar electrodialysis membranes was implemented to remove the unfavourable D-LA in the hydrolysate. This step allowed the reduction in LA concentration and subsequent fermentations of the hydrolysate resulted in enantiomeric purities over 98%. At the pilot scale, a fermentation of the pre-treated hydrolysate, by B. coagulans A166, resulted in a final LA concentration of 61.1 g L−1 and a yield of 0.94 g g−1. The downstream of the process resulted on a LA recovery of 51.5% and a L-LA optical purity of 98.7%.
- ItemDirect production of lactic acid based on simultaneous saccharification and fermentation of mixed restaurant food waste(Amsterdam : Elsevier, 2016) Pleissner, Daniel; Demichelis, Francesca; Mariano, Silvia; Fiore, Silvia; Gutiérrez, Ivette Michelle Navarro; Schneider, Roland; Venus, JoachimThis study introduces to a one-step process for the fermentative production of L(+)-lactic acid from mixed restaurant food waste. Food waste was used as carbon and nitrogen source in simultaneous saccharification and fermentation (SSF) using Lactobacillus sp. or Streptococcus sp. strains for L(+)-lactic acid production. Waste consisted of (w/w) 33.5% starch, 14.8% proteins, 12.9% fat and 8.5% free sugars. Lactobacillus sp. strains showed a productivity of 0.27–0.53 g L−1 h−1 and a yield of 0.07–0.14 g g−1 of theoretically available sugars, while Streptococcus sp. more efficiently degraded the food waste material and produced lactic acid at a maximum rate of 2.16 g L−1 h−1 and a yield of 0.81 g g−1. For SSF, no enzymes were added or other hydrolytic treatments were carried out. Outcomes revealed a linear relationship between lactic acid concentration and solid-to-liquid ratio when Streptococcus sp. was applied. Statistically, from a 20% (w/w) dry food waste blend 52.4 g L−1 lactic acid can be produced. Experimentally, 58 g L−1 was achieved in presence of 20% (w/w), which was the highest solid-to-liquid ratio that could be treated using the equipment applied. Irrespective if SSF was performed at laboratory or technical scale, or under non-sterile conditions, Streptococcus sp. efficiently liquefied food waste and converted the released nutrients directly into lactic acid without considerable production of other organic acids, such as acetic acid. Downstream processing including micro- and nanofiltration, electrodialysis, chromatography and distillation gave a pure 702 g L−1 L(+)-lactic acid formulation.