2020, Langer, Judith, de Aberasturi, Dorleta Jimenez, Aizpurua, Javier, Alvarez-Puebla, Ramon A., Auguié, Baptiste, Baumberg, Jeremy J., Bazan, Guillermo C., Bell, Steven E.J., Boisen, Anja, Brolo, Alexandre G., Choo, Jaebum, Cialla-May, Dana, Deckert, Volker, Fabris, Laura, Faulds, Karen, de Abajo, F. Javier García, Goodacre, Royston, Graham, Duncan, Haes, Amanda J., Haynes, Christy L., Huck, Christian, Itoh, Tamitake, Käll, Mikael, Kneipp, Janina, Kotov, Nicholas A., Kuang, Hua, Le Ru, Eric C., Lee, Hiang Kwee, Li, Jian-Feng, Ling, Xing Yi, Maier, Stefan A., Mayerhöfer, Thomas, Moskovits, Martin, Murakoshi, Kei, Nam, Jwa-Min, Nie, Shuming, Ozaki, Yukihiro, Pastoriza-Santos, Isabel, Perez-Juste, Jorge, Popp, Juergen, Pucci, Annemarie, Reich, Stephanie, Ren, Bin, Schatz, George C., Shegai, Timur, Schlücker, Sebastian, Tay, Li-Lin, Thomas, K. George, Tian, Zhong-Qun, Van Duyne, Richard P., Vo-Dinh, Tuan, Wang, Yue, Willets, Katherine A., Xu, Chuanlai, Xu, Hongxing, Xu, Yikai, Yamamoto, Yuko S., Zhao, Bing, Liz-Marzán, Luis M.

The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article.

2017, Vanhecke, Dimitri, Kuhn, Dagmar A., de Aberasturi, Dorleta Jimenez, Balog, Sandor, Milosevic, Ana, Urban, Dominic, Peckys, Diana, de Jonge, Niels, Parak, Wolfgang J., Petri-Fink, Alke, Rothen-Rutishauser, Barbara

Little is known about the simultaneous uptake of different engineered nanoparticle types, as it can be expected in our daily life. In order to test such co-exposure effects, murine macrophages (J774A.1 cell line) were incubated with gold (AuNPs) and iron oxide nanoparticles (FeOxNPs) either alone or combined. Environmental scanning electron microscopy revealed that single NPs of both types bound within minutes on the cell surface but with a distinctive difference between FeOxNPs and AuNPs. Uptake analysis studies based on laser scanning microscopy, transmission electron microscopy, and inductively coupled plasma optical emission spectrometry revealed intracellular appearance of both NP types in all exposure scenarios and a time-dependent increase. This increase was higher for both AuNPs and FeOxNPs during co-exposure. Cells treated with endocytotic inhibitors recovered after co-exposure, which additionally hinted that two uptake mechanisms are involved. Cross-talk between uptake pathways is relevant for toxicological studies: Co-exposure acts as an uptake accelerant. If the goal is to maximize the cellular uptake, e.g., for the delivery of pharmaceutical agents, this can be beneficial. However, co-exposure should also be taken into account in the case of risk assessment of occupational settings. The demonstration of co-exposure-invoked pathway interactions reveals that synergetic nanoparticle effects, either positive or negative, must be considered for nanotechnology and nanomedicine in particular to develop to its full potential.