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- ItemOxaliplatin-DNA adduct formation in white blood cells of cancer patients(London : Nature Publishing Group, 2008) Pieck, A.C.; Drescher, A.; Wiesmann, K.G.; Messerschmidt, J.; Weber, G.; Strumberg, D.; Hilger, R.A.; Scheulen, M.E.; Jaehde, U.In this study, we investigated the kinetics of oxaliplatin-DNA adduct formation in white blood cells of cancer patients in relation to efficacy as well as oxaliplatin-associated neurotoxicity. Thirty-seven patients with various solid tumours received 130 mg m−2 oxaliplatin as a 2-h infusion. Oxaliplatin-DNA adduct levels were measured in the first cycle using adsorptive stripping voltammetry. Platinum concentrations were measured in ultrafiltrate and plasma using a validated flameless atomic absorption spectrometry method. DNA adduct levels showed a characteristic time course, but were not correlated to platinum pharmacokinetics and varied considerably among individuals. In patients showing tumour response, adduct levels after 24 and 48 h were significantly higher than in nonresponders. Oxaliplatin-induced neurotoxicity was more pronounced but was not significantly different in patients with high adduct levels. The potential of oxaliplatin-DNA adduct measurements as pharmacodynamic end point should be further investigated in future trials.
- ItemSuccessful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis(London : BioMed Central, 2007) Carrol, Enitan D.; Salway, Fiona; Pepper, Stuart D.; Saunders, Emma; Mankhambo, Limangeni A.; Ollier, William E.; Hart, C. Anthony; Day, PhillipBackground: The challenge of gene expression studies is to reliably quantify levels of transcripts, but this is hindered by a number of factors including sample availability, handling and storage. The PAXgeneâ„¢ Blood RNA System includes a stabilizing additive in a plastic evacuated tube, but requires 2.5 mL blood, which makes routine implementation impractical for paediatric use. The aim of this study was to modify the PAXgeneâ„¢ Blood RNA System kit protocol for application to small, sick chidren, without compromising RNA integrity, and subsequently to perform quantitative analysis of ICAM and interleukin-6 gene expression. Aliquots of 0.86 mL PAXgeneâ„¢ reagent were put into microtubes and 0.3 mL whole blood added to maintain the same recommended proportions as in the PAXgeneâ„¢ evacuated tube system. RNA quality was assessed using the Agilent BioAnalyser 2100 and an in-house TaqManâ„¢ assay which measures GAPDH transcript integrity by determining 3' to 5' ratios. qPCR analysis was performed on an additional panel of 7 housekeeping genes. Three reference genes (HPRT1, YWHAZ and GAPDH) were identified using the GeNORM algorithm, which were subsequently used to normalising target gene expression levels. ICAM-1 and IL-6 gene expression were measured in 87 Malawian children with invasive pneumococcal disease. Results: Total RNA yield was between 1,114 and 2,950 ng and the BioAnalyser 2100 demonstrated discernible 18s and 28s bands. The cycle threshold values obtained for the seven housekeeping genes were between 15 and 30 and showed good consistency. Median relative ICAM and IL-6 gene expression were significantly reduced in non-survivors compared to survivors (ICAM: 3.56 vs 4.41, p = 0.04, and IL-6: 2.16 vs 6.73, p = 0.02). Conclusion: We have successfully modified the PAXgeneâ„¢ blood collection system for use in small children and demonstrated preservation of RNA integrity and successful quantitative real-time PCR analysis.
- ItemIntegration der Positronen-Emissions-Tomographie in die Strahlentherapie mit hochenergetischen Photonen(Dresden : Forschungszentrum Dresden-Rossendorf, 2009) Kunath, Daniela[no abstract available]
- ItemNeuartiges Konzept zum Tissue Engineering von langen Röhrenknochen auf der Basis biologisierter Scaffolds - Teilprojekt PHB-Fäden : (Unterprojekt Abt. MF-Erzeugung schmelzgesponnener PHB-Fäden für die textile Verarbeitung) ; Schlussbericht zum Projekt ; im Rahmen des Förderprogramms Innoregio/INNtex(Hannover : Technische Informationsbibliothek (TIB), 2007) Peitzsch, Lutz; Vogel, Roland; Brünig, Harald; Schmack, Gerhilt[no abstract available]
- ItemEntwicklung einer nicht-invasiven Diagnosemethode zur Diabetes-Früherkennung mittels Nah-Infrarot-Spektrometrie : Forschungsbericht zum Vorhaben ; BMBF-Programm "Innovationswettbewerb zur Förderung der Medizintechnik" ; Projekt: Diabetes-Früherkennung mittel NIR-Spektrometrie ; Laufzeit: 01.06.2002 - 30.09.2004(Hannover : Technische Informationsbibliothek (TIB), 2005) Heise, H.-Michael; Stücker, Markus; Ihrig, Dieter F.; Licht, Michael[no abstract available]
- ItemAnwendung des in-beam PET Therapiemonitorings auf Präzisionsbestrahlungen mit Helium-Ionen(Dresden : Forschungszentrum Dresden-Rossendorf, 2008) Fiedler, Fine[no abstract available]
- ItemSubstratinduzierte Veränderungen von Zellen im Kontakt mit Polymermaterialien : Schlussbericht(Dresden : Institut für Polymerforschung Dresden, 2002) IPF[no abstract available]
- ItemKompetenzzentrum für Materialien im Blut- und Gewebekontakt : Abschlussbericht(Hannover : Technische Informationsbibliothek (TIB), 2006) IPF[no abstract available]