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    Estimating Canopy Parameters Based on the Stem Position in Apple Trees Using a 2D LiDAR
    (Basel : MDPI AG, 2019) Tsoulias, Nikos; Paraforos, Dimitrios S.; Fountas, Spyros; Zude-Sasse, Manuela
    Data of canopy morphology are crucial for cultivation tasks within orchards. In this study, a 2D light detection and range (LiDAR) laser scanner system was mounted on a tractor, tested on a box with known dimensions (1.81 m × 0.6 m × 0.6 m), and applied in an apple orchard to obtain the 3D structural parameters of the trees (n = 224). The analysis of a metal box which considered the height of four sides resulted in a mean absolute error (MAE) of 8.18 mm with a bias (MBE) of 2.75 mm, representing a root mean square error (RMSE) of 1.63% due to gaps in the point cloud and increased incident angle with enhanced distance between laser aperture and the object. A methodology based on a bivariate point density histogram is proposed to estimate the stem position of each tree. The cylindrical boundary was projected around the estimated stem positions to segment each individual tree. Subsequently, height, stem diameter, and volume of the segmented tree point clouds were estimated and compared with manual measurements. The estimated stem position of each tree was defined using a real time kinematic global navigation satellite system, (RTK-GNSS) resulting in an MAE and MBE of 33.7 mm and 36.5 mm, respectively. The coefficient of determination (R2) considering manual measurements and estimated data from the segmented point clouds appeared high with, respectively, R2 and RMSE of 0.87 and 5.71% for height, 0.88 and 2.23% for stem diameter, as well as 0.77 and 4.64% for canopy volume. Since a certain error for the height and volume measured manually can be assumed, the LiDAR approach provides an alternative to manual readings with the advantage of getting tree individual data of the entire orchard.
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    Heat stress risk in European dairy cattle husbandry under different climate change scenarios – uncertainties and potential impacts
    (Göttingen : Copernicus, 2019) Hempel, Sabrina; Menz, Christoph; Pinto, Severino; Galán, Elena; Janke, David; Estellés, Fernando; Müschner-Siemens, Theresa; Wang, Xiaoshuai; Heinicke, Julia; Zhang, Guoqiang; Amon, Barbara; del Prado, Agustín; Amon, Thomas
    In the last decades, a global warming trend was observed. Along with the temperature increase, modifications in the humidity and wind regime amplify the regional and local impacts on livestock husbandry. Direct impacts include the occurrence of climatic stress conditions. In Europe, cows are economically highly relevant and are mainly kept in naturally ventilated buildings that are most susceptible to climate change. The high-yielding cows are particularly vulnerable to heat stress. Modifications in housing management are the main measures taken to improve the ability of livestock to cope with these conditions. Measures are typically taken in direct reaction to uncomfortable conditions instead of in anticipation of a long-term risk for climatic stress. Measures that balance welfare, environmental and economic issues are barely investigated in the context of climate change and are thus almost not available for commercial farms. Quantitative analysis of the climate change impacts on animal welfare and linked economic and environmental factors is rare. Therefore, we used a numerical modeling approach to estimate the future heat stress risk in such dairy cattle husbandry systems. The indoor climate was monitored inside three reference barns in central Europe and the Mediterranean regions. An artificial neuronal network (ANN) was trained to relate the outdoor weather conditions provided by official meteorological weather stations to the measured indoor microclimate. Subsequently, this ANN model was driven by an ensemble of regional climate model projections with three different greenhouse gas concentration scenarios. For the evaluation of the heat stress risk, we considered the number and duration of heat stress events. Based on the changes in the heat stress events, various economic and environmental impacts were estimated. The impacts of the projected increase in heat stress risk varied among the barns due to different locations and designs as well as the anticipated climate change (considering different climate models and future greenhouse gas concentrations). There was an overall increasing trend in number and duration of heat stress events. At the end of the century, the number of annual stress events can be expected to increase by up to 2000, while the average duration of the events increases by up to 22 h compared to the end of the last century. This implies strong impacts on economics, environment and animal welfare and an urgent need for mid-term adaptation strategies. We anticipated that up to one-tenth of all hours of a year, correspondingly one-third of all days, will be classified as critical heat stress conditions. Due to heat stress, milk yield may decrease by about 2.8 % relative to the present European milk yield, and farmers may expect financial losses in the summer season of about 5.4 % of their monthly income. In addition, an increasing demand for emission reduction measures must be expected, as an emission increase of about 16 Gg of ammonia and 0.1 Gg of methane per year can be expected under the anticipated heat stress conditions. The cattle respiration rate increases by up to 60 %, and the standing time may be prolonged by 1 h. This causes health issues and increases the probability of medical treatments. The various impacts imply feedback loops in the climate system which are presently underexplored. Hence, future in-depth studies on the different impacts and adaptation options at different stress levels are highly recommended.
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    Assessing the organic fraction of municipal solid wastes for the production of lactic acid
    (Amsterdam [u.a.] : Elsevier, 2019) López-Gómez, J. Pablo; Latorre-Sánchez, Marcos; Unger, Peter; Schneider, Roland; Coll Lozano, Caterina; Venus, Joachim
    With an estimated yearly production of about 140 Mt in the EU, conventionally, the organic fraction of municipal solid wastes (OFMSW) has been disposed in landfills with negative environmental effects. Nonetheless, the chemical composition of this residue make it a substrate with great bioconversion potential. In this study, OFMSW from Spanish municipal treatment plants, were evaluated for the production of LA. Samples were identified according to the sorting mechanisms employed for their collection in: (A) separately collected, (B) non-separately collected and (C) separately collected+paper/cardboard. Enzymatic hydrolysis was used to produce hydrolysates A, B and C accordingly. Hydrolysate A showed the highest total sugars and glucose content with values of 70 and 55 g·L−1, respectively. Following the characterisation, a screening showed that growth of B. coagulans was possible in all three hydrolysates. Furthermore, lab scale fermentations showed that LA final concentrations could reach around 60 g·L−1, with yields from total sugars of above 0.60 g·g−1. A technical scale fermentation of the hydrolysate A resulted in a final LA concentration of 60.7 g·L−1, a yield of 0.71 g·g−1 with a productivity of 2.68 g·L−1·h−1. Overall, it was estimated that 0.23 g of LA could be produced from one g of dry OFMSW.
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    Rapid determination of lime requirement by mid-infrared spectroscopy: A promising approach for precision agriculture
    (Weinheim : Wiley-VCH, 2019) Leenen, Matthias; Welp, Gerhard; Gebbers, Robin; Pätzold, Stefan
    Mid-infrared spectroscopy (MIRS) has proven to be a cost-effective, high throughput measurement technique for soil analysis. After multivariate calibration mid-infrared spectra can be used to predict various soil properties, some of which are related to lime requirement (LR). The objective of this study was to test the performance of MIRS for recommending variable rate liming on typical Central European soils in view of precision agriculture applications. In Germany, LR of arable topsoils is commonly derived from the parameters organic matter content (SOM), clay content, and soil pH (CaCl2) as recommended by the Association of German Agricultural Analytical and Research Institutes (VDLUFA). We analysed a total of 458 samples from six locations across Germany, which all revealed large within-field soil heterogeneity. Calcareous topsoils were observed at some positions of three locations (79 samples). To exclude such samples from LR determination, peak height at 2513 cm−1 of the MIR spectrum was used for identification. Spectra-based identification was accurate for carbonate contents > 0.5%. Subsequent LR derivation (LRSPP) from MIRS-PLSR predictions of SOM, clay, and pH (CaCl2) for non-calcareous soil samples using the VDLUFA look-up tables was successful for all locations (R2 = 0.54–0.82; RMSE = 857–1414 kg CaO ha−1). Alternatively, we tested direct LR prediction (LRDP) by MIRS-PLSR and also achieved satisfactory performance (R2 = 0.52–0.77; RMSE = 811–1420 kg CaO ha−1; RPD = 1.44–2.08). Further improvement was achieved by refining the VDLUFA tables towards a stepless algorithm. It can be concluded that MIRS provides a promising approach for precise LR estimation on heterogeneous arable fields. Large sample numbers can be processed with low effort which is an essential prerequisite for variable rate liming in precision agriculture. © 2019 The Authors. Journal of Plant Nutrition and Soil Science published by WILEY-VCH Verlag GmbH & Co. KGaA
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    Performance assessment of a solar dryer system using small parabolic dish and alumina/oil nanofluid: Simulation and experimental study
    (Basel : MDPI AG, 2019) Arkian, Amir Hossein; Najafi, Gholamhassan; Gorjian, Shiva; Loni, Reyhaneh; Bellos, Evangelos; Yusaf, Talal
    In this study, a small dish concentrator with a cylindrical cavity receiver was experimentally investigated as the heat source of a dryer. The system was examined for operation with pure thermal oil and Al2O3/oil nanofluid as the working fluids in the solar system. Moreover, the design, the development, and the evaluation of the dried mint plant are presented in this work. Also, the solar dryer system was simulated by the SolidWorks and ANSYS CFX software. On the other side, the color histogram of the wet and dried mint samples based on the RGB method was considered. The results revealed that the different temperatures of the solar working fluids at the inlet and outlet of the cavity receiver showed similar trend data compared to the variation of the solar radiation during the experimental test. Moreover, it is found that the cavity heat gain and thermal efficiency of the solar system was improved by using the nanofluid as the solar working fluid. Furthermore, the required time for mint drying had decreased by increasing the drying temperature and increasing air speed. The highest drying time was measured equal to 320 min for the condition of the air speed equal to 0.5 m/s and the drying temperature of 30 ◦C. A good agreement was observed between the calculated numerical results and measured experimental data. Finally, based on the color histogram of the wet and dried mint samples, it was concluded that intensity amount of the red color of the mint increased with the drying process compared to intensity amount of the red color of the wet mint sample. © 2019 by the authors.
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    The Impacts of Water Pricing and Non-Pricing Policies on Sustainable Water Resources Management: A Case of Ghorveh Plain at Kurdistan Province, Iran
    (Basel : MDPI, 2019) Asaadi, Mohammad Ali; Mortazavi, Seyed Abolghasem; Zamani, Omid; Najafi, Gholam Hassan; Yusaf, Talal; Hoseini, Seyed Salar
    As with other regions of Iran, due to excessive extraction of groundwater for intense agricultural activity, Ghorveh plain, a water-scarce irrigation district in the west of Iran, has faced a serious water crisis during the last decade. The present study investigates the impacts of two scenario policies, namely, non-price policy (as a supply-oriented policy) and water pricing policies (as a demand-oriented policy) on agricultural sector of Ghorveh Plain, using positive mathematical programming (PMP). The model was calibrated by using farm-level data for the crop years in 2016–2017. Our findings indicate that applying water supply constraint policy will change the land use and cropping pattern to the crops with higher water productivity. The increase of water resource constraints can lead to the increase of water economic return which indicates a rising value of water resources shortage, warning the producers of the agriculture sector to allocate water to the crops with higher economic value under the water resources shortage conditions. In addition, the findings underline that in a situation where the price of irrigation water is low due to the low elasticity of water demand in the agriculture sector, formulating the economic instruments such as rising water prices does not solely suffice to achieve sustainable water resource management. However, mixed scenarios emphasized that the water distribution policies should be aligned with the increases in water cost. © 2019 by the authors.
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    Relevance of interactions between starch-based coatings and plum fruit surfaces: A physical-chemical analysis
    (Basel : MDPI AG, 2019) Basiak, Ewelina; Geyer, Martin; Debeaufort, Frédéric; Lenart, Andrzej; Linke, Manfred
    In order to extend the shelf life of the fruit, improve appearance, and to keep all nutrition properties of the plum from diminishing, edible coatings comprised of wheat starch and wheat starch–whey protein isolate (in ratio 80/20) were created. Stand-alone films were produced to assess properties which helped to understand the phenomena occurring on the surface level of coated plums. The properties of coatings based on starch are similar to starch coatings containing oil because the natural epicuticular wax layer of plums merges with coating materials. Adding oil doubled the contact angle value and the dispersive component of the surface tension. The workings of adhesion and cohesion, spreading coefficient, water absorption, water content, and solubility in water of the films decreased. Similar processes were observed on the fruits’ surface. In appearance, the coating process is similar to polishing the plum surface for removing crystalline wax. The color parameters of coated fruits did not significantly change. Newly formed bonds or interactions established between starch, whey proteins, water, glycerol, and oil are displayed by Fourier transform infrared (FTIR) analysis. This work revealed how the interactions between the epicuticular wax on the fruit’s surface and the hydrocolloid-based coatings affect the efficiency of the coatings. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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    Comparison of ammonia emissions related to nitrogen use efficiency of livestock production in Europe
    (Amsterdam [u.a.] : Elsevier Science, 2019) Groenestein, C.M.; Hutchings, N.J.; Haenel, H.D.; Amon, B.; Menzi, H.; Mikkelsen, M.H.; Misselbrook, T.H.; van Bruggen, C.; Kupper, T.; Webb, J.
    The increasing global demand for food and the environmental effects of reactive nitrogen losses in the food production chain, increase the need for efficient use of nitrogen (N). Of N harvested in agricultural plant products, 80% is used to feed livestock. Because the largest atmospheric loss of reactive nitrogen from livestock production systems is ammonia (NH3), the focus of this paper is on N lost as NH3 during the production of animal protein. The focus of this paper is to understand the key factors explaining differences in Nitrogen Use Efficiency (NUE) of animal production among various European countries. Therefore we developed a conceptual framework to describe the NUE defined as the amount of animal-protein N per N in feed and NH3–N losses in the production of milk, beef, pork, chicken meat and eggs in The Netherlands, Switzerland, United Kingdom, Germany, Austria and Denmark. The framework describes how manure management and animal-related parameters (feed, metabolism) relate to NH3 emissions and NUE. The results showed that the animal product with the lowest NUE had the largest NH3 emissions and vice versa, which agrees with the reciprocal relationship between NUE and NH3 within the conceptual framework. Across animal products for the countries considered, about 20% of the N in feed is lost as NH3. The significant smallest proportion (12%) of NH3–N per unit of Nfeed is from chicken production. The proportions for other products are 17%, 19%, 20% and 22% for milk, pork, eggs and beef respectively. These differences were not significantly different due to the differences among countries. For all countries, NUE was lowest for beef and highest for chicken. The production of 1 kg N in beef required about 5 kg N in feed, of which 1 kg N was lost as NH3–N. For the production of 1 kg N in chicken meat, 2 kg N in feed was required and 0.2 kg was lost as NH3. The production of 1 kg N in milk required 4 kg N in feed with 0.6 kg NH3–N loss, the same as pork and eggs, but those needed 3 and 3.5 kg N in feed per kg N in product respectively. Except for beef, the differences among these European countries were mainly caused by differences in manure management practices and their emission factors, rather than by animal-related factors including feed and digestibility influencing the excreted amount of ammoniacal N (TAN). For beef, both aspects caused important differences. Based on the results, we encourage the expression of N losses as per N in feed or per N in product, in addition to per animal place, when comparing production efficiency and NUE. We consider that disaggregating emission factors into a diet/animal effect and a manure management effect would improve the basis for comparing national NH3 emission inventories. © 2018 The Authors
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    Methane prediction based on individual or groups of milk fatty acids for dairy cows fed rations with or without linseed
    (New York, NY [u.a.] : Elsevier, 2019) Engelke, Stefanie W.; Daş, Gürbüz; Derno, Michael; Tuchscherer, Armin; Wimmers, Klaus; Rychlik, Michael; Kienberger, Hermine; Berg, Werner; Kuhla, Björn; Metges, Cornelia C.
    Milk fatty acids (MFA) are a proxy for the prediction of CH4 emission from cows, and prediction differs with diet. Our objectives were (1) to compare the effect of diets on the relation between MFA profile and measured CH4 production, (2) to predict CH4 production based on 6 data sets differing in the number and type of MFA, and (3) to test whether additional inclusion of energy-corrected milk (ECM) yield or dry matter intake (DMI) as explanatory variables improves predictions. Twenty dairy cows were used. Four diets were used based on corn silage (CS) or grass silage (GS) without (L0) or with linseed (LS) supplementation. Ten cows were fed CS-L0 and CS-LS and the other 10 cows were fed GS-L0 and GS-LS in random order. In feeding wk 5 of each diet, CH4 production (L/d) was measured in respiration chambers for 48 h and milk was analyzed for MFA concentrations by gas chromatography. Specific CH4 prediction equations were obtained for L0-, LS-, GS-, and CS-based diets and for all 4 diets collectively and validated by an internal cross-validation. Models were developed containing either 43 identified MFA or a reduced set of 7 groups of biochemically related MFA plus C16:0 and C18:0. The CS and LS diets reduced CH4 production compared with GS and L0 diets, respectively. Methane yield (L/kg of DMI) reduction by LS was higher with CS than GS diets. The concentrations of C18:1 trans and n-3 MFA differed among GS and CS diets. The LS diets resulted in a higher proportion of unsaturated MFA at the expense of saturated MFA. When using the data set of 43 individual MFA to predict CH4 production (L/d), the cross-validation coefficient of determination (R2 CV) ranged from 0.47 to 0.92. When using groups of MFA variables, the R2 CV ranged from 0.31 to 0.84. The fit parameters of the latter models were improved by inclusion of ECM or DMI, but not when added to the data set of 43 MFA for all diets pooled. Models based on GS diets always had a lower prediction potential (R2 CV = 0.31 to 0.71) compared with data from CS diets (R2 CV = 0.56 to 0.92). Models based on LS diets produced lower prediction with data sets with reduced MFA variables (R2 CV = 0.62 to 0.68) compared with L0 diets (R2 CV = 0.67 to 0.80). The MFA C18:1 cis-9 and C24:0 and the monounsaturated FA occurred most often in models. In conclusion, models with a reduced number of MFA variables and ECM or DMI are suitable for CH4 prediction, and CH4 prediction equations based on diets containing linseed resulted in lower prediction accuracy. © 2019 American Dairy Science Association
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    Metaproteome analysis reveals that syntrophy, competition, and phage-host interaction shape microbial communities in biogas plants
    (London : Biomed Central, 2019) Heyer, R.; Schallert, K.; Siewert, C.; Kohrs, F.; Greve, J.; Maus, I.; Klang, J.; Klocke, M.; Heiermann, M.; Hoffmann, M.; Püttker, S.; Calusinska, M.; Zoun, R.; Saake, G.; Benndorf, D.; Reichl, U.
    Background: In biogas plants, complex microbial communities produce methane and carbon dioxide by anaerobic digestion of biomass. For the characterization of the microbial functional networks, samples of 11 reactors were analyzed using a high-resolution metaproteomics pipeline. Results: Examined methanogenesis archaeal communities were either mixotrophic or strictly hydrogenotrophic in syntrophy with bacterial acetate oxidizers. Mapping of identified metaproteins with process steps described by the Anaerobic Digestion Model 1 confirmed its main assumptions and also proposed some extensions such as syntrophic acetate oxidation or fermentation of alcohols. Results indicate that the microbial communities were shaped by syntrophy as well as competition and phage-host interactions causing cell lysis. For the families Bacillaceae, Enterobacteriaceae, and Clostridiaceae, the number of phages exceeded up to 20-fold the number of host cells. Conclusion: Phage-induced cell lysis might slow down the conversion of substrates to biogas, though, it could support the growth of auxotrophic microbes by cycling of nutrients. © 2019 The Author(s).