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    Influence of Tree Species, Harvesting Method and Storage on Energy Demand and Wood Chip Quality When Chipping Poplar, Willow and Black Locust
    (Basel : MDPI AG, 2020) Pecenka, Ralf; Lenz, Hannes; Jekayinfa, Simeon Olatayo; Hoffmann, Thomas
    The cultivation of fast-growing wood (e.g., poplar, willow or black locust) in short rotation coppices and agroforestry systems presents an opportunity for producing biomass sustainably in the agricultural sector. Cost-efficient agricultural wood production requires the availability of high-performance machinery and methods with which high-quality wood chips can be produced at low cost. It is known from harvesting short rotation coppices in practice that both the wood chip quality and the performance of the harvesting machinery depend on a variety of factors (e.g., harvesting method, weather conditions, tree species). That is why this study examines in detail the influence of the tree species (different varieties of poplar, willow, black locust) and the wood condition (fresh, stored or dried, frozen) on the specific energy demand for comminution in a stationary drum chipper and on the particle size distribution of the wood chips produced. For all the tree species examined, the chipping of dried as well as frozen stems was connected with a significant increase in the specific energy demand for comminution. An increase of 31% has been measured if poplar stems are chipped in frozen conditions (max. 6.31 kWh t−1). Drying led to an increase of 59% for dried willow stems (max. 6.67 kWh t−1). Drying and frost had also an influence on the size and quality of the wood chips, but no globally significant connection could be established for the examined tree varieties.
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    Using SPOT-7 for Nitrogen Fertilizer Management in Oil Palm
    (Basel : MDPI AG, 2020) Yadegari, Mohammad; Shamshiri, Redmond R.; Shariff, Abdul Rashid Mohamed; Balasundram, Siva K.; Mahns, Benjamin
    Environmental concerns are growing about excessive applying nitrogen (N) fertilizers, especially in oil palm. Some conventional methods which are used to assess the amount of nutrient in oil palm are time-consuming, expensive, and involve frond destruction. Remote sensing as a non-destructive, affordable, and efficient method is widely used to detect the concentration of chlorophyll (Chl) from canopy plants using several vegetation indices (VIs) because there is an influential relation between the concentration of N in the leaves and canopy Chl content. The objectives of this research are to (i) evaluate and compare the performance of various vegetation indices (VIs) for measuring N status in oil palm canopy using SPOT-7 imagery (AIRBUS Defence & Space, Ottobrunn, Germany) to (ii) develop a regression formula that can predict the N content using satellite data to (iii) assess the regression formula performance on testing datasets by testing the coefficient of determination between the predicted and measured N contents. SPOT-7 was acquired in a 6-ha oil palm planted area in Pahang, Malaysia. To predict N content, 28 VIs based on the spectral range of SPOT-7 satellite images were evaluated. Several regression models were applied to determine the highest coefficient of determination between VIs and actual N content from leaf sampling. The modified soil-adjusted vegetation index (MSAVI) generated the highest coefficient of determination (R2 = 0.93). MTVI1 and triangular VI had the highest second and third coefficient of determination with N content (R2 = 0.926 and 0.923, respectively). The classification accuracy assessment of the developed model was evaluated using several statistical parameters such as the independent t-test, and p-value. The accuracy assessment of the developed model was more than 77%.
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    Prospects and challenges of translational corneal bioprinting
    (Basel : MDPI AG, 2020) Fuest, Matthias; Yam, Gary Hin-Fai; Mehta, Jodhbir S.; Campos, Daniela F.Duarte
    Corneal transplantation remains the ultimate treatment option for advanced stromal and endothelial disorders. Corneal tissue engineering has gained increasing interest in recent years, as it can bypass many complications of conventional corneal transplantation. The human cornea is an ideal organ for tissue engineering, as it is avascular and immune-privileged. Mimicking the complex mechanical properties, the surface curvature, and stromal cytoarchitecure of the in vivo corneal tissue remains a great challenge for tissue engineering approaches. For this reason, automated biofabrication strategies, such as bioprinting, may offer additional spatial control during the manufacturing process to generate full-thickness cell-laden 3D corneal constructs. In this review, we discuss recent advances in bioprinting and biomaterials used for in vitro and ex vivo corneal tissue engineering, corneal cell-biomaterial interactions after bioprinting, and future directions of corneal bioprinting aiming at engineering a full-thickness human cornea in the lab. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Batch and continuous lactic acid fermentation based on a multi-substrate approach
    (Basel : MDPI AG, 2020) Olszewska-Widdrat, Agata; Alexandri, Maria; López-Gómez, José Pablo; Schneider, Roland; Venus, Joachim
    The utilisation of waste materials and industrial residues became a priority within the bioeconomy concept and the production of biobased chemicals. The aim of this study was to evaluate the feasibility to continuously produce L-lactic acid from different renewable substrates, in a multi-substrate strategy mode. Based on batch experiments observations, Bacillus coagulans A534 strain was able to continuously metabolise acid whey, sugar beet molasses, sugar bread, alfalfa press green juice and tapioca starch. Additionally, reference experiments showed its behaviour in standard medium. Continuous fermentations indicated that the highest productivity was achieved when molasses was employed with a value of 10.34 g·L−1·h−1, while the lactic acid to sugar conversion yield was 0.86 g·g−1 . This study demonstrated that LA can be efficiently produced in continuous mode regardless the substrate, which is a huge advantage in comparison to other platform chemicals. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    The role of petrimonas mucosa ING2-E5at in mesophilic biogas reactor systems as deduced from multiomics analyses
    (Basel : MDPI AG, 2020) Maus, Irena; Tubbesing, Tom; Wibberg, Daniel; Heyer, Robert; Hassa, Julia; Tomazetto, Geizecler; Huang, Liren; Bunk, Boyke; Spröer, Cathrin; Benndorf, Dirk; Zverlov, Vladimir; Pühler, Alfred; Klocke, Michael; Sczyrba, Alexander; Schlüter, Andreas
    Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes
    (Basel : MDPI AG, 2020) Klang, Johanna; Szewzyk, Ulrich; Bock, Daniel; Theuerl, Susanne
    In this study the response of biogas-producing microbiomes to a profound feedstock change was investigated. The microbiomes were adapted to the digestion of either 100% sugar beet, maize silage, or of the silages with elevated amounts of total ammonium nitrogen (TAN) by adding ammonium carbonate or animal manure. The feedstock exchange resulted in a short-range decrease or increase in the biogas yields according to the level of chemical feedstock complexity. Fifteen taxa were found in all reactors and can be considered as generalists. Thirteen taxa were detected in the reactors operated with low TAN and six in the reactors with high TAN concentration. Taxa assigned to the phylum Bacteroidetes and to the order Spirochaetales increased with the exchange to sugar beet silage, indicating an affinity to easily degradable compounds. The recorded TAN-sensitive taxa (phylum Cloacimonetes) showed no specific affinity to maize or sugar beet silage. The archaeal community remained unchanged. The reported findings showed a smooth adaptation of the microbial communities, without a profound negative impact on the overall biogas production indicating that the two feedstocks, sugar beet and maize silage, potentially do not contain chemical compounds that are difficult to handle during anaerobic digestion.
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    Valorising agro-industrial wastes within the circular bioeconomy concept: The case of defatted rice bran with emphasis on bioconversion strategies
    (Basel : MDPI AG, 2020) Alexandri, Maria; López-Gómez, José Pablo; Olszewska-Widdrat, Agata; Venus, Joachim
    The numerous environmental problems caused by the extensive use of fossil resources have led to the formation of the circular bioeconomy concept. Renewable resources will constitute the cornerstone of this new, sustainable model, with biomass presenting a huge potential for the production of fuels and chemicals. In this context, waste and by-product streams from the food industry will be treated not as "wastes" but as resources. Rice production generates various by-product streams which currently are highly unexploited, leading to environmental problems especially in the countries that are the main producers. The main by-product streams include the straw, the husks, and the rice bran. Among these streams, rice bran finds applications in the food industry and cosmetics, mainly due to its high oil content. The high demand for rice bran oil generates huge amounts of defatted rice bran (DRB), the main by-product of the oil extraction process. The sustainable utilisation of this by-product has been a topic of research, either as a food additive or via its bioconversion into value-added products and chemicals. This review describes all the processes involved in the efficient bioconversion of DRB into biotechnological products. The detailed description of the production process, yields and productivities, as well as strains used for the production of bioethanol, lactic acid and biobutanol, among others, are discussed. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Engineering robust cellulases for tailored lignocellulosic degradation cocktails
    (Basel : MDPI AG, 2020) Contreras, Francisca; Pramanik, Subrata; Rozhkova, Aleksandra M.; Zorov, Ivan N.; Korotkova, Olga; Sinitsyn, Arkady P.; Schwaneberg, Ulrich; Davari, Mehdi D.
    Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass requires a synergistic action of several cellulases and hemicellulases. Cellulases depolymerize cellulose, the main polymer of the lignocellulosic biomass, to its building blocks. The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of bioethanol. The main challenges include low activity, product inhibition, and the need to perform fine-tuning of a cellulase cocktail for each type of biomass. Protein engineering and directed evolution are powerful technologies to improve enzyme properties such as increased activity, decreased product inhibition, increased thermal stability, improved performance in non-conventional media, and pH stability, which will lead to a production of more efficient cocktails. In this review, we focus on recent advances in cellulase cocktail production, its current challenges, protein engineering as an efficient strategy to engineer cellulases, and our view on future prospects in the generation of tailored cellulases for biofuel production. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Impact of process parameters and bulk properties on quality of dried hops
    (Basel : MDPI AG, 2020) Raut, Sharvari; Gersdorff, Gardis J.E. von; Münsterer, Jakob; Kammhuber, Klaus; Hensel, Oliver; Sturm, Barbara
    Hops are critical to the brewing industry. In commercial hop drying, a large bulk of hops is dried in multistage kilns for several hours. This affects the drying behavior and alters the amount and chemical composition of the hop oils. To understand these changes, hops of the var. Hallertauer Tradition were dried in bulks of 15, 25 and 35 kg/m2 at 60◦C and 0.35 m/s. Additionally, bulks of 25 kg/m2 were also dried at 65◦C and 0.45 m/s to assess the effect of change in temperature and velocity, respectively. The results obtained show that bulk weights significantly influence the drying behavior. Classification based on the cone size reveals 45.4% medium cones, 41.2% small cones and 8.6% large cones. The highest ∆E value of 6.3 and specific energy consumption (113,476 kJ/kgH2O) were observed for the 15 kg/m2 bulk. Increasing the temperature from 60◦C to 65◦C increased the oil yield losses by about 7% and myrcene losses by 22%. The results obtained show that it is important to define and consider optimum bulk and process parameters, to optimize the hop drying process to improve the process efficiency as well the product quality. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Microbiome diversity and community-level change points within manure-based small biogas plants
    (Basel : MDPI AG, 2020) Theuerl, Susanne; Klang, Johanna; Hülsemann, Benedikt; Mächtig, Torsten; Hassa, Julia
    Efforts to integrate biogas plants into bioeconomy concepts will lead to an expansion of manure-based (small) biogas plants, while their operation is challenging due to critical characteristics of some types of livestock manure. For a better process understanding, in this study, three manure-based small biogas plants were investigated with emphasis on microbiome diversity. Due to varying digester types, feedstocks, and process conditions, 16S rRNA gene amplicon sequencing showed differences in the taxonomic composition. Dynamic variations of each investigated biogas plant microbiome over time were analyzed by terminal restriction fragment length polymorphism (TRFLP), whereby nonmetric multidimensional scaling (NMDS) revealed two well-running systems, one of them with a high share of chicken manure, and one unstable system. By using Threshold Indicator Taxa Analysis (TITAN), community-level change points at ammonium and ammonia concentrations of 2.25 g L-1 and 193 mg L-1 or volatile fatty acid concentrations of 0.75 g L-1were reliably identified which are lower than the commonly reported thresholds for critical process stages based on chemical parameters. Although a change in the microbiome structure does not necessarily indicate an upcoming critical process stage, the recorded community-level change points might be a first indication to carefully observe the process. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.