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- ItemOn the Buoyancy Subrange in Stratified Turbulence(Basel : MDPI AG, 2020) Avsarkisov, VictorThis study is motivated by the importance of the stratified turbulence in geophysical flows. We present a theoretical analysis of the buoyancy subrange based on the theory of strongly stratified turbulence. Some important turbulent scales and their relations are explored. Scaling constants of the buoyancy subrange scaling laws for both kinetic and potential energy spectra are derived and analyzed. It is found that these constants are functions of the horizontal Froude number Frh . For the potential energy spectrum, the scaling constant also depends on the turbulent flux coefficient of Γ .
- ItemEffectivity and Cost Efficiency of a Tax on Nitrogen Fertilizer to Reduce GHG Emissions from Agriculture(Basel : MDPI AG, 2020) Meyer-Aurich, Andreas; Nadi Karatay, Yusuf; Nausediene, Ausra; Kirschke, DieterThe use of nitrogen (N) fertilizer substantially contributes to greenhouse gas (GHG) emissions due to N2O emissions from agricultural soils and energy-intensive fertilizer manufacturing. Thus, a reduction of mineral N fertilizer use can contribute to reduced GHG emissions. Fertilizer tax is a potential instrument to provide incentives to apply less fertilizer and contribute to the mitigation of GHG emissions. This study provides model results based on a production function analysis from field experiments in Brandenburg and Schleswig-Holstein, with respect to risk aversion by calculating certainty equivalents for different levels of risk aversion. The model results were used to identify effective and cost-efficient options considering farmers’ risk aversion to reduce N fertilizer, and to compare the potential and cost of GHG mitigation with different N fertilizer tax schemes. The results show that moderate N tax levels are effective in reducing N fertilizer levels, and thus, in curbing GHG emissions at costs below 100 €/t CO2eq for rye, barley and canola. However, in wheat production, N tax has limited effects on economically optimal N use due to the effects of N fertilizer on crop quality, which affect the sale prices of wheat. The findings indicate that the level of risk aversion does not have a consistent impact on the reduction of N fertilizer with a tax, even though the level of N fertilizer use is generally lower for risk-averse agents. The differences in N fertilizer response might have an impact on the relative advantage of different crops, which should be taken into account for an effective implementation of a tax on N fertilizer.
- ItemInfluence of Tree Species, Harvesting Method and Storage on Energy Demand and Wood Chip Quality When Chipping Poplar, Willow and Black Locust(Basel : MDPI AG, 2020) Pecenka, Ralf; Lenz, Hannes; Jekayinfa, Simeon Olatayo; Hoffmann, ThomasThe cultivation of fast-growing wood (e.g., poplar, willow or black locust) in short rotation coppices and agroforestry systems presents an opportunity for producing biomass sustainably in the agricultural sector. Cost-efficient agricultural wood production requires the availability of high-performance machinery and methods with which high-quality wood chips can be produced at low cost. It is known from harvesting short rotation coppices in practice that both the wood chip quality and the performance of the harvesting machinery depend on a variety of factors (e.g., harvesting method, weather conditions, tree species). That is why this study examines in detail the influence of the tree species (different varieties of poplar, willow, black locust) and the wood condition (fresh, stored or dried, frozen) on the specific energy demand for comminution in a stationary drum chipper and on the particle size distribution of the wood chips produced. For all the tree species examined, the chipping of dried as well as frozen stems was connected with a significant increase in the specific energy demand for comminution. An increase of 31% has been measured if poplar stems are chipped in frozen conditions (max. 6.31 kWh t−1). Drying led to an increase of 59% for dried willow stems (max. 6.67 kWh t−1). Drying and frost had also an influence on the size and quality of the wood chips, but no globally significant connection could be established for the examined tree varieties.
- ItemUsing SPOT-7 for Nitrogen Fertilizer Management in Oil Palm(Basel : MDPI AG, 2020) Yadegari, Mohammad; Shamshiri, Redmond R.; Shariff, Abdul Rashid Mohamed; Balasundram, Siva K.; Mahns, BenjaminEnvironmental concerns are growing about excessive applying nitrogen (N) fertilizers, especially in oil palm. Some conventional methods which are used to assess the amount of nutrient in oil palm are time-consuming, expensive, and involve frond destruction. Remote sensing as a non-destructive, affordable, and efficient method is widely used to detect the concentration of chlorophyll (Chl) from canopy plants using several vegetation indices (VIs) because there is an influential relation between the concentration of N in the leaves and canopy Chl content. The objectives of this research are to (i) evaluate and compare the performance of various vegetation indices (VIs) for measuring N status in oil palm canopy using SPOT-7 imagery (AIRBUS Defence & Space, Ottobrunn, Germany) to (ii) develop a regression formula that can predict the N content using satellite data to (iii) assess the regression formula performance on testing datasets by testing the coefficient of determination between the predicted and measured N contents. SPOT-7 was acquired in a 6-ha oil palm planted area in Pahang, Malaysia. To predict N content, 28 VIs based on the spectral range of SPOT-7 satellite images were evaluated. Several regression models were applied to determine the highest coefficient of determination between VIs and actual N content from leaf sampling. The modified soil-adjusted vegetation index (MSAVI) generated the highest coefficient of determination (R2 = 0.93). MTVI1 and triangular VI had the highest second and third coefficient of determination with N content (R2 = 0.926 and 0.923, respectively). The classification accuracy assessment of the developed model was evaluated using several statistical parameters such as the independent t-test, and p-value. The accuracy assessment of the developed model was more than 77%.
- ItemBase Neutralizing Capacity of Agricultural Soils in a Quaternary Landscape of North-East Germany and Its Relationship to Best Management Practices in Lime Requirement Determination(Basel : MDPI AG, 2020) Vogel, Sebastian; Bönecke, Eric; Kling, Charlotte; Kramer, Eckart; Lück, Katrin; Nagel, Anne; Philipp, Golo; Rühlmann, Jörg; Schröter, Ingmar; Gebbers, RobinDespite being a natural soil-forming process, soil acidification is a major agronomic challenge under humid climate conditions, as soil acidity influences several yield-relevant soil properties. It can be counterbalanced by the regular application of agricultural lime to maintain or re-establish soil fertility and to optimize plant growth and yield. To avoid underdose as well as overdose, lime rates need to be calculated carefully. The lime rate should be determined by the optimum soil pH (target pH) and the response of the soil to lime, which is described by the base neutralizing capacity (BNC). Several methods exist to determine the lime requirement (LR) to raise the soil pH to its optimum. They range from extremely time-consuming equilibration methods, which mimic the natural processes in the soil, to quick tests, which rely on some approximations and are designed to provide farmers with timely and cost-efficient data. Due to the higher analytical efforts, only limited information is available on the real BNC of particular soils. In the present paper, we report the BNC of 420 topsoil samples from Central Europe (north-east Germany), developed on sediments from the last ice age 10,000 years ago under Holocene conditions. These soils are predominantly sandy and low in humus, but they exhibit a huge spatial variability in soil properties on a small scale. The BNC was determined by adding various concentrations of Ca(OH)2 and fitting an exponential model to derive a titration curve for each sample. The coefficients of the BNC titration curve were well correlated with soil properties affecting soil acidity and pH buffer capacity, i.e., pH, soil texture and soil organic matter (SOM). From the BNC model, the LRs (LRBNC) were derived and compared with LRVDLUFA based on the standard protocol in Germany as established by the Association of German Agricultural Analytic and Research Institutes (VDLUFA). The LRBNC and LRVDLUFA correlated well but the LRVDLUFA were generally by approximately one order of magnitude higher. This is partly due to the VDLUFA concept to recommend a maintenance or conservation liming, even though the pH value is in the optimum range, to keep it there until the next lime application during the following rotation. Furthermore, the VDLUFA method was primarily developed from field experiments where natural soil acidification and management practices depressed the effect of lime treatment. The BNC method, on the other hand, is solely based on laboratory analysis with standardized soil samples. This indicates the demand for further research to develop a sound scientific algorithm that complements LRBNC with realistic values of annual Ca2+ removal and acidification by natural processes and N fertilization.
- ItemDesign, implementation, evaluation and application of a 32-channel radio frequency signal generator for thermal magnetic resonance based anti-cancer treatment(Basel : MDPI AG, 2020) Han, Haopeng; Eigentler, Thomas Wilhelm; Wang, Shuailin; Kretov, Egor; Winter, Lukas; Hoffmann, Werner; Grass, Eckhard; Niendorf, ThoralfThermal Magnetic Resonance (ThermalMR) leverages radio frequency (RF)-induced heating to examine the role of temperature in biological systems and disease. To advance RF heating with multi-channel RF antenna arrays and overcome the shortcomings of current RF signal sources, this work reports on a 32-channel modular signal generator (SGPLL). The SGPLL was designed around phase-locked loop (PLL) chips and a field-programmable gate array chip. To examine the system properties, switching/settling times, accuracy of RF power level and phase shifting were characterized. Electric field manipulation was successfully demonstrated in deionized water. RF heating was conducted in a phantom setup using self-grounded bow-tie RF antennae driven by the SGPLL. Commercial signal generators limited to a lower number of RF channels were used for comparison. RF heating was evaluated with numerical temperature simulations and experimentally validated with MR thermometry. Numerical temperature simulations and heating experiments controlled by the SGPLL revealed the same RF interference patterns. Upon RF heating similar temperature changes across the phantom were observed for the SGPLL and for the commercial devices. To conclude, this work presents the first 32-channel modular signal source for RF heating. The large number of coherent RF channels, wide frequency range and accurate phase shift provided by the SGPLL form a technological basis for ThermalMR controlled hyperthermia anti-cancer treatment. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemPlasma treatment limits cutaneous squamous cell carcinoma development in vitro and in vivo(Basel : MDPI AG, 2020) Pasqual-Melo, Gabriella; Nascimento, Thiago; Sanches, Larissa Juliani; Blegniski, Fernanda Paschoal; Bianchi, Julya Karen; Sagwal, Sanjeev Kumar; Berner, Julia; Schmidt, Anke; Emmert, Steffen; Weltmann, Klaus-Dieter; Woedtke, Thomas von; Gandhirajan, Rajesh Kumar; Cecchini, Alessandra Lourenço; Bekeschus, SanderCutaneous squamous cell carcinoma (SCC) is the most prevalent cancer worldwide, increasing the cost of healthcare services and with a high rate of morbidity. Its etiology is linked to chronic ultraviolet (UV) exposure that leads to malignant transformation of keratinocytes. Invasive growth and metastasis are severe consequences of this process. Therapy-resistant and highly aggressive SCC is frequently fatal, exemplifying the need for novel treatment strategies. Cold physical plasma is a partially ionized gas, expelling therapeutic doses of reactive oxygen and nitrogen species that were investigated for their anticancer capacity against SCC in vitro and SCC-like lesions in vivo. Using the kINPen argon plasma jet, a selective growth-reducing action of plasma treatment was identified in two SCC cell lines in 2D and 3D cultures. In vivo, plasma treatment limited the progression of UVB-induced SSC-like skin lesions and dermal degeneration without compromising lesional or non-lesional skin. In lesional tissue, this was associated with a decrease in cell proliferation and the antioxidant transcription factor Nrf2 following plasma treatment, while catalase expression was increased. Analysis of skin adjacent to the lesions and determination of global antioxidant parameters confirmed the local but not systemic action of the plasma anticancer therapy in vivo. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemIdentification of two kinase inhibitors with synergistic toxicity with low-dose hydrogen peroxide in colorectal cancer cells in vitro(Basel : MDPI AG, 2020) Freund, Eric; Liedtke, Kim-Rouven; Miebach, Lea; Wende, Kristian; Heidecke, Amanda; Kaushik, Nagendra Kumar; Choi, Eun Ha; Partecke, Lars-Ivo; Bekeschus, SanderColorectal carcinoma is among the most common types of cancers. With this disease, diffuse scattering in the abdominal area (peritoneal carcinosis) often occurs before diagnosis, making surgical removal of the entire malignant tissue impossible due to a large number of tumor nodules. Previous treatment options include radiation and its combination with intraperitoneal heat-induced chemotherapy (HIPEC). Both options have strong side effects and are often poor in therapeutic efficacy. Tumor cells often grow and proliferate dysregulated, with enzymes of the protein kinase family often playing a crucial role. The present study investigated whether a combination of protein kinase inhibitors and low-dose induction of oxidative stress (using hydrogen peroxide, H2O2) has an additive cytotoxic effect on murine, colorectal tumor cells (CT26). Protein kinase inhibitors from a library of 80 substances were used to investigate colorectal cancer cells for their activity, morphology, and immunogenicity (immunogenic cancer cell death, ICD) upon mono or combination. Toxic compounds identified in 2D cultures were confirmed in 3D cultures, and additive cytotoxicity was identified for the substances lavendustin A, GF109203X, and rapamycin. Toxicity was concomitant with cell cycle arrest, but except HMGB1, no increased expression of immunogenic markers was identified with the combination treatment. The results were validated for GF109203X and rapamycin but not lavendustin A in the 3D model of different colorectal (HT29, SW480) and pancreatic cancer cell lines (MiaPaca, Panc01). In conclusion, our in vitro data suggest that combining oxidative stress with chemotherapy would be conceivable to enhance antitumor efficacy in HIPEC. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemCombination treatment with cold physical plasma and pulsed electric fields augments ros production and cytotoxicity in lymphoma(Basel : MDPI AG, 2020) Wolff, Christina M.; Kolb, Juergen F.; Weltmann, Klaus-Dieter; Woedtke, Thomas von; Bekeschus, SanderNew approaches in oncotherapy rely on the combination of different treatments to enhance the efficacy of established monotherapies. Pulsed electric fields (PEFs) are an established method (electrochemotherapy) for enhancing cellular drug uptake while cold physical plasma is an emerging and promising anticancer technology. This study aimed to combine both technologies to elucidate their cytotoxic potential as well as the underlying mechanisms of the effects observed. An electric field generator (0.9–1.0 kV/cm and 100-μs pulse duration) and an atmospheric pressure argon plasma jet were employed for the treatment of lymphoma cell lines as a model system. PEF but not plasma treatment induced cell membrane permeabilization. Additive cytotoxicity was observed for the metabolic activity and viability of the cells while the sequence of treatment in the combination played only a minor role. Intriguingly, a parallel combination was more effective compared to a 15-min pause between both treatment regimens. A combination effect was also found for lipid peroxidation; however, none could be observed in the cytosolic and mitochondrial reactive oxygen species (ROS) production. The supplementation with either antioxidant, a pan-caspase-inhibitor or a ferroptosis inhibitor, all partially rescued lymphoma cells from terminal cell death, which contributes to the mechanistic understanding of this combination treatment. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- ItemLong-term Risk Assessment for Medical Application of Cold Atmospheric Pressure Plasma(Basel : MDPI AG, 2020) Rutkowski, Rico; Daeschlein, Georg; Woedtke, Thomas von; Smeets, Ralf; Gosau, Martin; Metelmann, Hans-RobertDespite increasing knowledge gained based on multidisciplinary research, plasma medicine still raises various questions regarding specific effects as well as potential risks. With regard to significant statements about in vivo applicability that cannot be prognosticated exclusively based on in vitro data, there is still a deficit of clinical data. This study included a clinical follow-up of five probands who had participated five years previously in a study on the influence of cold atmospheric pressure plasma (CAP) on the wound healing of CO2 laser-induced skin lesions. The follow-up included a complex imaging diagnostic involving dermatoscopy, confocal laser scanning microscopy (CLSM) and hyperspectral imaging (HSI). Hyperspectral analysis showed no relevant microcirculatory differences between plasma-treated and non-plasma-treated areas. In summary of all the findings, no malignant changes, inflammatory reactions or pathological changes in cell architecture could be detected in the plasma-treated areas. These unique in vivo long-term data contribute to a further increase in knowledge about important safety aspects in regenerative plasma medicine. However, to confirm these findings and secure indication-specific dose recommendations, further clinical studies are required. © 2020 by the authors.