Search Results
A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits
Neuromuscular circuits (NMCs) are vital for voluntary movement, and effective models of NMCs are needed to understand the pathogenesis of, as well as to identify effective treatments for, multiple diseases, including Duchenne's muscular dystrophy and amyotrophic lateral sclerosis. Microfluidics are ideal for recapitulating the central and peripheral compartments of NMCs, but myotubes often detach before functional NMCs are formed. In addition, microfluidic systems are often limited to a single experimental unit, which significantly limits their application in disease modeling and drug discovery. Here, we developed a microfluidic platform (MFP) containing over 100 experimental units, making it suitable for medium-throughput applications. To overcome detachment, we incorporated a reactive polymer surface allowing customization of the environment to culture different cell types. Using this approach, we identified conditions that enable long-term co-culture of human motor neurons and myotubes differentiated from human induced pluripotent stem cells inside our MFP. Optogenetics demonstrated the formation of functional NMCs. Furthermore, we developed a novel application of the rabies tracing assay to efficiently identify NMCs in our MFP. Therefore, our MFP enables large-scale generation and quantification of functional NMCs for disease modeling and pharmacological drug targeting. © 2019 The Authors
Effect of pore geometry on ultra-densified hydrogen in microporous carbons
Our investigations into molecular hydrogen (H2) confined in microporous carbons with different pore geometries at 77 K have provided detailed information on effects of pore shape on densification of confined H2 at pressures up to 15 MPa. We selected three materials: a disordered, phenolic resin-based activated carbon, a graphitic carbon with slit-shaped pores (titanium carbide-derived carbon), and single-walled carbon nanotubes, all with comparable pore sizes of <1 nm. We show via a combination of in situ inelastic neutron scattering studies, high-pressure H2 adsorption measurements, and molecular modelling that both slit-shaped and cylindrical pores with a diameter of ∼0.7 nm lead to significant H2 densification compared to bulk hydrogen under the same conditions, with only subtle differences in hydrogen packing (and hence density) due to geometric constraints. While pore geometry may play some part in influencing the diffusion kinetics and packing arrangement of hydrogen molecules in pores, pore size remains the critical factor determining hydrogen storage capacities. This confirmation of the effects of pore geometry and pore size on the confinement of molecules is essential in understanding and guiding the development and scale-up of porous adsorbents that are tailored for maximising H2 storage capacities, in particular for sustainable energy applications.
The impact of atmospheric boundary layer, opening configuration and presence of animals on the ventilation of a cattle barn
Naturally ventilated livestock buildings (NVLB) represent one of the most significant sources of ammonia emissions. However, even the dispersion of passive gas in an NVLB is still not well understood. In this paper, we present a detailed investigation of passive pollutant dispersion in a model of a cattle barn using the wind tunnel experiment method. We simulated the pollution of the barn by a ground-level planar source. We used the time-resolved particle image velocimetry (TR-PIV) and the fast flame ionisation detector (FFID) to study the flow and dispersion processes at high spatial and temporal resolution. We employed the Proper Orthogonal Decomposition (POD) and Oscillating Patterns Decomposition (OPD) methods to detect the coherent structures of the flow. The results show that the type of atmospheric boundary layer (ABL) and sidewall opening height have a significant impact on the pollutant dispersion in the barn, while the presence of animals and doors openings are insignificant under conditions of winds perpendicular to the sidewall openings. We found that the dynamic coherent structures, developed by the Kelvin-Helmholtz instability, contribute to the pollutant transport in the barn. We demonstrate that in any of the studied cases the pollutant was not well mixed within the barn and that a significant underestimation (up to by a factor 3) of the barn ventilation might be obtained using, e.g. tracer gas method. © 2020 The Authors
S-wave seismic imaging of near-surface sediments using tailored processing strategies
Reflection seismic imaging using horizontally-polarized S-waves (SH) can increase resolution and it could be cost-efficient compared to the common use of P-waves. However, since S-wave application often delivers varying data quality, appropriate processing schemes are required for particular imaging and interpretation purposes. In this paper, we present four tailored processing strategies that are applied to SH-wave data acquired in an overdeepened Quaternary basin in the Alpine foreland, the Tannwald Basin. The applied processing schemes consist of (1) processing using a short automatic gain control window that enhances structural details and highlights small-scale structures, (2) offset restriction indicating that relative small offsets are sufficient for adequate imaging, which offers reduced field operation costs, (3) coherency-enhancement that reveals large-scale structures for interpretation, and (4) adapted amplitude scaling that enables structural comparison of P-wave and S-wave seismic sections. With respect to P-wave data measured on the same profile, we demonstrate the benefits of the S-wave seismic reflection method. P-waves offer robust imaging results, but S-waves double the resolution, better depict shallow reflections, and may image reflectors in areas where the P-wave struggles. At least for the Tannwald Basin, S-wave imaging is also more cost-efficient than P-wave imaging. © 2020 The Authors
A survey on Bluetooth multi-hop networks
Bluetooth was firstly announced in 1998. Originally designed as cable replacement connecting devices in a point-to-point fashion its high penetration arouses interest in its ad-hoc networking potential. This ad-hoc networking potential of Bluetooth is advertised for years - but until recently no actual products were available and less than a handful of real Bluetooth multi-hop network deployments were reported. The turnaround was triggered by the release of the Bluetooth Low Energy Mesh Profile which is unquestionable a great achievement but not well suited for all use cases of multi-hop networks. This paper surveys the tremendous work done on Bluetooth multi-hop networks during the last 20 years. All aspects are discussed with demands for a real world Bluetooth multi-hop operation in mind. Relationships and side effects of different topics for a real world implementation are explained. This unique focus distinguishes this survey from existing ones. Furthermore, to the best of the authors’ knowledge this is the first survey consolidating the work on Bluetooth multi-hop networks for classic Bluetooth technology as well as for Bluetooth Low Energy. Another individual characteristic of this survey is a synopsis of real world Bluetooth multi-hop network deployment efforts. In fact, there are only four reports of a successful establishment of a Bluetooth multi-hop network with more than 30 nodes and only one of them was integrated in a real world application - namely a photovoltaic power plant. © 2019 The Authors
On the geometric stability of an inorganic nanowire and an organic ligand shell
The break-up of a nanowire with an organic ligand shell into discrete droplets is analysed in terms of the Rayleigh-Plateau instability. Explicit account is taken of the effect of the organic ligand shell upon the energetics and kinetics of surface diffusion in the wire. Both an initial perturbation analysis and a full numerical analysis of the evolution in wire morphology are conducted, and the governing non-dimensional groups are identified. The perturbation analysis is remarkably accurate in obtaining the main features of the instability, including the pinch-off time and the resulting diameter of the droplets. It is conjectured that the surface energy of the wire and surrounding organic shell depends upon both the mean and deviatoric invariants of the curvature tensor. Such a behaviour allows for the possibility of a stable nanowire such that the Rayleigh-Plateau instability is not energetically favourable. A stability map illustrates this. Maps are also constructed for the final droplet size and pinch-off time as a function of two non-dimensional groups that characterise the energetics and kinetics of diffusion in the presence of the organic shell. These maps can guide future experimental activity on the stabilisation of nanowires by organic ligand shells. © 2018 The Authors
A new method to measure real-world respiratory tract deposition of inhaled ambient black carbon
In this study, we present the development of a mobile system to measure real-world total respiratory tract deposition of inhaled ambient black carbon (BC). Such information can be used to supplement the existing knowledge on air pollution-related health effects, especially in the regions where the use of standard methods and intricate instrumentation is limited. The study is divided in two parts. Firstly, we present the design of portable system and methodology to evaluate the exhaled air BC content. We demonstrate that under real-world conditions, the proposed system exhibit negligible particle losses, and can additionally be used to determine the minute ventilation. Secondly, exemplary experimental data from the system is presented. A feasibility study was conducted in the city of La Paz, Bolivia. In a pilot experiment, we found that the cumulative total respiratory tract deposition dose over 1-h commuting trip would result in approximately 2.6 μg of BC. This is up to 5 times lower than the values obtained from conjectural approach (e.g. using physical parameters from previously reported worksheets). Measured total respiratory tract deposited BC fraction varied from 39% to 48% during walking and commuting inside a micro-bus, respectively. To the best of our knowledge, no studies focusing on experimental determination of real-world deposition dose of BC have been performed in developing regions. This can be especially important because the BC mass concentration is significant and determines a large fraction of particle mass concentration. In this work, we propose a potential method, recommendations, as well as the limitations in establishing an easy and relatively cheap way to estimate the respiratory tract deposition of BC. In this study we present a novel method to measure real-world respiratory tract deposition dose of Black Carbon. Results from a pilot study in La Paz, Bolivia, are presented. © 2019 The Authors
Monitoring the thermally induced transition from sp3-hybridized into sp2-hybridized carbons
The preparation of carbons for technical applications is typically based on a treatment of a precursor, which is transformed into the carbon phase with the desired structural properties. During such treatment the material passes through several different structural stages, for example, starting from precursor molecules via an amorphous phase into crystalline-like phases. While the structure of non-graphitic and graphitic carbon has been well studied, the transformation stages from molecular to amorphous and non-graphitic carbon are still not fully understood. Disordered carbon often contains a mixture of sp3-, sp2-and sp1-hybridized bonds, whose analysis is difficult to interpret. We systematically address this issue by studying the transformation of purely sp3-hybridized carbons, that is, nanodiamond and adamantane, into sp2-hybridized non-graphitic and graphitic carbon. The precursor materials are thermally treated at different temperatures and the transformation stages are monitored. We employ Raman spectroscopy, WAXS and TEM to characterize the structural changes. We correlate the intensities and positions of the Raman bands with the lateral crystallite size La estimated by WAXS analysis. The behavior of the D and G Raman bands characteristic for sp2-type material formed by transforming the sp3-hybridized precursors into non-graphitic and graphitic carbon agrees well with that observed using sp2-structured precursors.
Breakdown of continuum models for spherical probe adhesion tests on micropatterned surfaces
The adhesion of fibrillar dry adhesives, mimicking nature's principles of contact splitting, is commonly characterized by using axisymmetric probes having either a flat punch or spherical geometry. When using spherical probes, the adhesive pull-off force measured depends strongly on the compressive preload applied when making contact and on the geometry of the probe. Together, these effects complicate comparisons of the adhesive performance of micropatterned surfaces measured in different experiments. In this work we explore these issues, extending previous theoretical treatments of this problem by considering a fully compliant backing layer with an array of discrete elastic fibrils on its surface. We compare the results of the semi-analytical model presented to existing continuum theories, particularly with respect to determining a measurement system- and procedure-independent metric for the local adhesive strength of the fibrils from the global pull-off force. It is found that the discrete nature of the interface plays a dominant role across a broad range of relevant system parameters. Accordingly, a convenient tool for simulation of a discrete array is provided. An experimental procedure is recommended for use in conjunction with this tool in order to extract a value for the local adhesive strength of the fibrils, which is independent of the other system properties (probe radius, backing layer thickness, and preload) and thus is suitable for comparison across experimental studies.
Probability of success studies for geothermal projects in clastic reservoirs: From subsurface data to geological risk analysis
In the realisation of a geothermal project, an important step is the quantification of the geological risk of a well not achieving the economically necessary cut-off values with respect to temperature and flowrate/drawdown. In this paper, we present a new method for calculating this risk via a probability of success study by using all available types of hydraulic data, including porosity values derived from core samples or borehole logs. This method has been developed for geothermal projects in fluvial sandstones of the North German Basin but can be applied to any clastic, not fracture-dominated reservoir worldwide. © 2019 The Authors