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- ItemNitrous oxide emissions from winter oilseed rape cultivation(Amsterdam [u.a.] : Elsevier, 2017) Ruser, Reiner; Fuß, Roland; Andres, Monique; Hegewald, Hannes; Kesenheimer, Katharina; Köbke, Sarah; Räbiger, Thomas; Quinones, Teresa Suarez; Augustin, Jürgen; Christen, Olaf; Dittert, Klaus; Kage, Henning; Lewandowski, Iris; Prochnow, Annette; Stichnothe, Heinz; Flessa, HeinzWinter oilseed rape (Brassica napus L., WOSR) is the major oil crop cultivated in Europe. Rapeseed oil is predominantly used for production of biodiesel. The framework of the European Renewable Energy Directive requires that use of biofuels achieves GHG savings of at least 50% compared to use of fossil fuel starting in 2018. However, N2O field emissions are estimated using emission factors that are not specific for the crop and associated with strong uncertainty. N2O field emissions are controlled by N fertilization and dominate the GHG balance of WOSR cropping due to the high global warming potential of N2O. Thus, field experiments were conducted to increase the data basis and subsequently derive a new WOSR-specific emission factor. N2O emissions and crop yields were monitored for three years over a range of N fertilization intensities at five study sites representative of German WOSR production. N2O fluxes exhibited the typical high spatial and temporal variability in dependence on soil texture, weather and nitrogen availability. The annual N2O emissions ranged between 0.24 kg and 5.48 kg N2O-N ha−1 a−1. N fertilization increased N2O emissions, particularly with the highest N treatment (240 kg N ha−1). Oil yield increased up to a fertilizer amount of 120 kg N ha−1, higher N-doses increased grain yield but decreased oil concentrations in the seeds. Consequently oil yield remained constant at higher N fertilization. Since, yield-related emission also increased exponentially with N surpluses, there is potential for reduction of the N fertilizer rate, which offers perspectives for the mitigation of GHG emissions. Our measurements double the published data basis of annual N2O flux measurements in WOSR. Based on this extended dataset we modeled the relationship between N2O emissions and fertilizer N input using an exponential model. The corresponding new N2O emission factor was 0.6% of applied fertilizer N for a common N fertilizer amount under best management practice in WOSR production (200 kg N ha−1 a−1). This factor is substantially lower than the linear IPCC Tier 1 factor (EF1) of 1.0% and other models that have been proposed. © 2017
- ItemDNA and RNA extraction and quantitative real-time PCR-based assays for biogas biocenoses in an interlaboratory comparison(Basel : MDPI, 2016) Lebuhn, Michael; Derenkó, Jaqueline; Rademacher, Antje; Helbig, Susanne; Munk, Bernhard; Pechtl, Alexander; Stolze, Yvonne; Prowe, Steffen; Schwarz, Wolfgang H.; Schlüter, Andreas; Liebl, Wolfgang; Klocke, MichaelFive institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extraction system based on physical and chemical lysis with excellent extraction efficiency yielded highly reproducible results among the partners and clearly outperformed a traditional CTAB/chloroform/isoamylalcohol based method. Analytical purpose, sample texture, consistency and upstream pretreatment steps determine the modifications that should be applied to achieve maximum efficiency in the trade-off between extract purity and nucleic acid recovery rate. RNA extraction was much more variable, and the destination of the extract determines the method to be used. RNA stabilization with quaternary ammonium salts was an as satisfactory approach as flash freezing in liquid N2. Due to co-eluted impurities, spectrophotometry proved to be of limited value for nucleic acid qualification and quantification in extracts obtained with the kit, and picoGreen® based quantification was more trustworthy. Absorbance at 230 nm can be extremely high in the presence of certain chaotropic guanidine salts, but guanidinium isothiocyanate does not affect (q)PCR. Absolute quantification by qPCR requires application of a reliable internal standard for which correct PCR efficiency and Y-intercept values are important and must be reported.
- ItemUncertainty in the measurement of indoor temperature and humidity in naturally ventilated dairy buildings as influenced by measurement technique and data variability(Amsterdam : Elsevier, 2017) Hempel, Sabrina; König, Marcel; Menz, Christoph; Janke, David; Amon, Barbara; Banhazi, Thomas M.; Estellés, Fernando; Amon, ThomasThe microclimatic conditions in dairy buildings affect animal welfare and gaseous emissions. Measurements are highly variable due to the inhomogeneous distribution of heat and humidity sources (related to farm management) and the turbulent inflow (associated with meteorologic boundary conditions). The selection of the measurement strategy (number and position of the sensors) and the analysis methodology adds to the uncertainty of the applied measurement technique. To assess the suitability of different sensor positions, in situations where monitoring in the direct vicinity of the animals is not possible, we collected long-term data in two naturally ventilated dairy barns in Germany between March 2015 and April 2016 (horizontal and vertical profiles with 10 to 5 min temporal resolution). Uncertainties related to the measurement setup were assessed by comparing the device outputs under lab conditions after the on-farm experiments. We found out that the uncertainty in measurements of relative humidity is of particular importance when assessing heat stress risk and resulting economic losses in terms of temperature-humidity index. Measurements at a height of approximately 3 m–3.5 m turned out to be a good approximation for the microclimatic conditions in the animal occupied zone (including the air volume close to the emission active zone). However, further investigation along this cross-section is required to reduce uncertainties related to the inhomogeneous distribution of humidity. In addition, a regular sound cleaning (and if possible recalibration after few months) of the measurement devices is crucial to reduce the instrumentation uncertainty in long-term monitoring of relative humidity in dairy barns. © 2017 The Authors
- ItemEstimating Canopy Parameters Based on the Stem Position in Apple Trees Using a 2D LiDAR(Basel : MDPI AG, 2019) Tsoulias, Nikos; Paraforos, Dimitrios S.; Fountas, Spyros; Zude-Sasse, ManuelaData of canopy morphology are crucial for cultivation tasks within orchards. In this study, a 2D light detection and range (LiDAR) laser scanner system was mounted on a tractor, tested on a box with known dimensions (1.81 m × 0.6 m × 0.6 m), and applied in an apple orchard to obtain the 3D structural parameters of the trees (n = 224). The analysis of a metal box which considered the height of four sides resulted in a mean absolute error (MAE) of 8.18 mm with a bias (MBE) of 2.75 mm, representing a root mean square error (RMSE) of 1.63% due to gaps in the point cloud and increased incident angle with enhanced distance between laser aperture and the object. A methodology based on a bivariate point density histogram is proposed to estimate the stem position of each tree. The cylindrical boundary was projected around the estimated stem positions to segment each individual tree. Subsequently, height, stem diameter, and volume of the segmented tree point clouds were estimated and compared with manual measurements. The estimated stem position of each tree was defined using a real time kinematic global navigation satellite system, (RTK-GNSS) resulting in an MAE and MBE of 33.7 mm and 36.5 mm, respectively. The coefficient of determination (R2) considering manual measurements and estimated data from the segmented point clouds appeared high with, respectively, R2 and RMSE of 0.87 and 5.71% for height, 0.88 and 2.23% for stem diameter, as well as 0.77 and 4.64% for canopy volume. Since a certain error for the height and volume measured manually can be assumed, the LiDAR approach provides an alternative to manual readings with the advantage of getting tree individual data of the entire orchard.
- ItemExpressing stemflow commensurate with its ecohydrological importance(Amsterdam [u.a.] : Elsevier Science, 2018) Carlyle-Moses, Darryl E.; Iida, Shin'ichi; Germer, Sonja; Llorens, Pilar; Michalzik, Beate; Nanko, Kazuki; Tischer, Alexander; Levia, Delphis F.Despite some progress, the importance of stemflow remains obscured partly due to computations emphasizing canopy interception loss. We advocate for two metrics—the stand-scale funneling ratio and the stand-scale infiltration funneling ratio—to more accurately portray stemflow inputs and increase comparability across ecosystems. These metrics yield per unit area stemflow inputs orders of magnitude greater than what would have been delivered by throughfall or precipitation alone. We recommend that future studies employ these stand-scale funnelling metrics to express stemflow commensurate with its ecohydrological importance and better conceptualize the role of stemflow in plant-soil interactions, permitting advances in critical zone science. © 2018 The Authors
- ItemThe MiLA tool: Modeling greenhouse gas emissions and cumulative energy demand of energy crop cultivation in rotation(Amsterdam : Elsevier, 2016) Peter, Christiane; Specka, Xenia; Aurbacher, Joachim; Peter Kornatz, Peter Kornatz; Herrmann, Christiane; Heiermann, Monika; Müller, Janine; Nendel, ClaasCrop rotations are part of current agricultural practice, since they and their effects can contribute to a sustainable agricultural cropping system. However, in current Life Cycle Assessment (LCA) studies, crop rotation effects are insufficiently considered, since these effects are difficult to measure. LCA studies from crop production typically take only one vegetation period into account. As a result, the consideration of how the assessed crop is influenced by the previous crop (crop rotation effects) including: (1) nutrient carryover, (2) reduction in operational requirements and (3) different intensity and timing of farming activities, is outside of the system boundary. However, ignoring these effects may lead to incorrect interpretation of LCA results and consequently to poor agricultural management as well as poor policy decisions. A new LCA tool called the “Model for integrative Life Cycle Assessment in Agriculture (MiLA)” is presented in this work. MiLA has been developed to assess GHG emissions and cumulative energy demands (CED) of cropping systems by taking the characteristics of crop cultivation in rotation into account. This tool enables the user to analyze cropping systems at farm level in order to identify GHG mitigation options and energy-efficient cropping systems. The tool was applied to a case study, including two crop rotations in two different regions in Germany with the goal of demonstrating the effectiveness of this tool on LCA results. Results show that including crop rotation effects can influence the GHG emission result of the individual crop by − 34% up to + 99% and the CED by − 16 up to + 89%. Expanding the system boundary by taking the whole crop rotation into account as well as providing the results based on different functional units improves LCA of energy crop production and helps those making the assessment to draw a more realistic picture of the interactions between crops while increasing the reliability of the LCA results.
- ItemAn alternative to field retting: Fibrous materials based on wet preserved hemp for the manufacture of composites(Basel : MDPI AG, 2019) Gusovius, H.-J.; Lühr, C.; Hoffmann, T.; Pecenka, R.; Idler, C.A process developed at the Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB) for the supply and processing of wet-preserved fiber plants opens up new potential uses for such resources. The processing of industrial hemp into fiber materials and products thereof is undergoing experimental research along the value-added chain from the growing process through to the manufacturing of product samples. The process comprises the direct harvesting of the field-fresh hemp and the subsequent anaerobic storage of the entire plant material. Thus, process risk due to unfavorable weather conditions is prevented in contrast to common dew retting procedures. The effects of the anaerobic storage processes on the properties of the bast part of the plant material are comparable to the results of common retting procedures. Harvest storage, as well as further mechanical processing, leads to different geometrical properties compared to the bast fibers resulting from traditional post harvesting treatment and decortication. The fiber raw material obtained in this way is well suited to the production of fiberboards and the reinforcement of polymer or mineral bonded composites. The objective of this paper is to present recent research results on final products extended by a comprehensive overview of the whole supply chain in order to enable further understanding of the result influencing aspects of prior process steps.
- ItemMilk fatty acids estimated by mid-infrared spectroscopy and milk yield can predict methane emissions in dairy cows(Berlin ; Heidelberg : Springer, 2018-5-2) Engelke, Stefanie W.; Daş, Gürbüz; Derno, Michael; Tuchscherer, Armin; Berg, Werner; Kuhla, Björn; Metges, Cornelia C.Ruminant enteric methane emission contributes to global warming. Although breeding low methane-emitting cows appears to be possible through genetic selection, doing so requires methane emission quantification by using elaborate instrumentation (respiration chambers, SF6 technique, GreenFeed) not feasible on a large scale. It has been suggested that milk fatty acids are promising markers of methane production. We hypothesized that methane emission can be predicted from the milk fatty acid concentrations determined by mid-infrared spectroscopy, and the integration of energy-corrected milk yield would improve the prediction. Therefore, we examined relationships between methane emission of cows measured in respiration chambers and milk fatty acids, predicted by mid-infrared spectroscopy, to derive diet-specific and general prediction equations based on milk fatty acid concentrations alone and with the additional consideration of energy-corrected milk yield. Cows were fed diets differing in forage type and linseed supplementation to generate a large variation in both CH4 emission and milk fatty acids. Depending on the diet, equations derived from regression analysis explained 61 to 96% of variation of methane emission, implying the potential of milk fatty acid data predicted by mid-infrared spectroscopy as novel proxy for direct methane emission measurements. When data from all diets were analyzed collectively, the equation with energy-corrected milk yield (CH4 (L/day) = − 1364 + 9.58 × energy-corrected milk yield + 18.5 × saturated fatty acids + 32.4 × C18:0) showed an improved coefficient of determination of cross-validation R2 CV = 0.72 compared to an equation without energy-corrected milk yield (R2 CV = 0.61). Equations developed for diets supplemented by linseed showed a lower R2 CV as compared to diets without linseed (0.39 to 0.58 vs. 0.50 to 0.91). We demonstrate for the first time that milk fatty acid concentrations predicted by mid-infrared spectroscopy together with energy-corrected milk yield can be used to estimate enteric methane emission in dairy cows. © 2018, The Author(s).
- ItemRisk efficiency of irrigation to cereals in northeast Germany with respect to nitrogen fertilizer(Amsterdam : Elsevier, 2016) Meyer-Aurich, Andreas; Gandorfer, Markus; Trost, Benjamin; Ellmer, Frank; Baumecker, MichaelThe potential role of irrigation of cereals as a response to climate change is under debate. Especially under temperate continental conditions empirical evidence of crop yield response to irrigation in interaction with nitrogen fertilizer supply is rare. Besides mean yield effects, irrigation reduces yield variance, which may be an incentive for farmers to use irrigation. This paper investigates the risk-efficiency of irrigation in cereal production in a temperate continental climate, based on data from a long term field experiment on a sandy soil. Irrigation and no irrigation of winter rye (Secale cereale) and winter barley (Hordeum vulgare) were investigated in three different nitrogen (N) fertilizer levels. Crop yield response data (1995–2010) to irrigation and N fertilizer were used to calculate net returns, certainty equivalents (CE) for different levels of risk aversion and the conditional value at risk (CVaR) as a downside risk indicator in two price scenarios. The scenarios were calculated with a total cost and a partial budget approach. Irrigation was found to be profit-maximizing in all partial budget calculations, which sometimes required higher N input to be profit-maximizing. Irrigation and N fertilizer reduction were identified as risk mitigation strategies, even though their impact was limited. Irrigation reduced the downside risk only in the partial budget calculations. The analysis based on the CE did not show improved risk efficiency with irrigated management options. In contrast, reduced fertilizer input proved to be risk efficient at specific levels of risk aversion. The price expectations of winter rye and winter barley had a much higher impact on the ranking of the management options than risk aversion based on the crop yield variances. At low crop prices for all levels of risk aversion, irrigation of winter barley and winter rye was only economically justified if fixed costs for irrigation were not taken into account. At high crop prices, irrigation of winter barley was also justified based on the total cost calculation. However, this advantage was only given at a very low level of risk aversion. With increasing levels of risk aversion irrigation was not efficient based on the CE in the total cost accounting scenario. In conclusion, irrigation of cereals can contribute to downside risk mitigation and increased profits, if fixed costs for irrigation are covered. However, this conclusion holds only when irrigation is combined with an increased N intensity. If total costs need to be accounted for, irrigation in cereals is not an appropriate risk reduction strategy and a reduction of N input is more effective.
- ItemBiochar research activities and their relation to development and environmental quality. A meta-analysis(Berlin ; Heidelberg : Springer, 2017-6-6) Mehmood, Khalid; Chávez Garcia, Elizabeth; Schirrmann, Michael; Ladd, Brenton; Kammann, Claudia; Wrage-Mönnig, Nicole; Siebe, Christina; Estavillo, Jose M.; Fuertes-Mendizabal, Teresa; Cayuela, Mariluz; Sigua, Gilbert; Spokas, Kurt; Cowie, Annette L.; Novak, Jeff; Ippolito, James A.; Borchard, NilsBiochar is the solid product that results from pyrolysis of organic materials. Its addition to highly weathered soils changes physico-chemical soil properties, improves soil functions and enhances crop yields. Highly weathered soils are typical of humid tropics where agricultural productivity is low and needs to be raised to reduce human hunger and poverty. However, impact of biochar research on scientists, politicians and end-users in poor tropical countries remains unknown; assessing needs and interests on biochar is essential to develop reliable knowledge transfer/translation mechanisms. The aim of this publication is to present results of a meta-analysis conducted to (1) survey global biochar research published between 2010 and 2014 to assess its relation to human development and environmental quality, and (2) deduce, based on the results of this analysis, priorities required to assess and promote the role of biochar in the development of adapted and sustainable agronomic methods. Our main findings reveal for the very first time that: (1) biochar research associated with less developed countries focused on biochar production technologies (26.5 ± 0.7%), then on biochars’ impact on chemical soil properties (18.7 ± 1.2%), and on plant productivity (17.1 ± 2.6%); (2) China dominated biochar research activities among the medium developed countries focusing on biochar production technologies (26.8 ± 0.5%) and on use of biochar as sorbent for organic and inorganic compounds (29.1 ± 0.4%); and (3) the majority of biochar research (69.0±2.9%) was associated with highly developed countries that are able to address a higher diversity of questions. Evidently, less developed countries are eager to improve soil fertility and agricultural productivity, which requires transfer and/or translation of biochar knowledge acquired in highly developed countries. Yet, improving local research capacities and encouraging synergies across scientific disciplines and countries are crucial to foster development of sustainable agronomy in less developed countries. © 2017, The Author(s).