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End date: 2021 × Start date: 2020 × DDC: 624 ×

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    Superelasticity of Plasma- and Synthetic Membranes Resulting from Coupling of Membrane Asymmetry, Curvature, and Lipid Sorting
    (Weinheim : Wiley-VCH, 2021) Steinkühler, Jan; Fonda, Piermarco; Bhatia, Tripta; Zhao, Ziliang; Leomil, Fernanda S. C.; Lipowsky, Reinhard; Dimova, Rumiana
    Biological cells are contained by a fluid lipid bilayer (plasma membrane, PM) that allows for large deformations, often exceeding 50% of the apparent initial PM area. Isolated lipids self-organize into membranes, but are prone to rupture at small (<2–4%) area strains, which limits progress for synthetic reconstitution of cellular features. Here, it is shown that by preserving PM structure and composition during isolation from cells, vesicles with cell-like elasticity can be obtained. It is found that these plasma membrane vesicles store significant area in the form of nanotubes in their lumen. These act as lipid reservoirs and are recruited by mechanical tension applied to the outer vesicle membrane. Both in experiment and theory, it is shown that a “superelastic” response emerges from the interplay of lipid domains and membrane curvature. This finding allows for bottom-up engineering of synthetic biomaterials that appear one magnitude softer and with threefold larger deformability than conventional lipid vesicles. These results open a path toward designing superelastic synthetic cells possessing the inherent mechanics of biological cells.
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    Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice
    (Weinheim : Wiley-VCH, 2021) Clemen, Ramona; Freund, Eric; Mrochen, Daniel; Miebach, Lea; Schmidt, Anke; Rauch, Bernhard H.; Lackmann, Jan‐Wilm; Martens, Ulrike; Wende, Kristian; Lalk, Michael; Delcea, Mihaela; Bröker, Barbara M.; Bekeschus, Sander
    Reactive oxygen species (ROS/RNS) are produced during inflammation and elicit protein modifications, but the immunological consequences are largely unknown. Gas plasma technology capable of generating an unmatched variety of ROS/RNS is deployed to mimic inflammation and study the significance of ROS/RNS modifications using the model protein chicken ovalbumin (Ova vs oxOva). Dynamic light scattering and circular dichroism spectroscopy reveal structural modifications in oxOva compared to Ova. T cells from Ova-specific OT-II but not from C57BL/6 or SKH-1 wild type mice presents enhanced activation after Ova addition. OxOva exacerbates this activation when administered ex vivo or in vivo, along with an increased interferon-gamma production, a known anti-melanoma agent. OxOva vaccination of wild type mice followed by inoculation of syngeneic B16F10 Ova-expressing melanoma cells shows enhanced T cell number and activation, decreased tumor burden, and elevated numbers of antigen-presenting cells when compared to their Ova-vaccinated counterparts. Analysis of oxOva using mass spectrometry identifies three hot spots regions rich in oxidative modifications that are associated with the increased T cell activation. Using Ova as a model protein, the findings suggest an immunomodulating role of multi-ROS/RNS modifications that may spur novel research lines in inflammation research and for vaccination strategies in oncology.
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    In Situ Fabrication of Freestanding Single-Atom-Thick 2D Metal/Metallene and 2D Metal/ Metallene Oxide Membranes: Recent Developments
    (Weinheim : Wiley-VCH, 2021) Ta, Huy Q.; Mendes, Rafael G.; Liu, Yu; Yang, Xiaoqin; Luo, Jingping; Bachmatiuk, Alicja; Gemming, Thomas; Zeng, Mengqi; Fu, Lei; Liu, Lijun; Rümmeli, Mark H.
    In recent years, two-dimensional (2D) materials have attracted a lot of research interest as they exhibit several fascinating properties. However, outside of 2D materials derived from van der Waals layered bulk materials only a few other such materials are realized, and it remains difficult to confirm their 2D freestanding structure. Despite that, many metals are predicted to exist as 2D systems. In this review, the authors summarize the recent progress made in the synthesis and characterization of these 2D metals, so called metallenes, and their oxide forms, metallene oxides as free standing 2D structures formed in situ through the use of transmission electron microscopy (TEM) and scanning TEM (STEM) to synthesize these materials. Two primary approaches for forming freestanding monoatomic metallic membranes are identified. In the first, graphene pores as a means to suspend the metallene or metallene oxide and in the second, electron-beam sputtering for the selective etching of metal alloys or thick complex initial materials is employed to obtain freestanding single-atom-thick 2D metal. The data show a growing number of 2D metals/metallenes and 2D metal/ metallene oxides having been confirmed and point to a bright future for further discoveries of these 2D materials.
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    Chemokine‐Capturing Wound Contact Layer Rescues Dermal Healing
    (Weinheim : Wiley-VCH, 2021) Schirmer, Lucas; Atallah, Passant; Freudenberg, Uwe; Werner, Carsten
    Excessive inflammation often impedes the healing of chronic wounds. Scavenging of chemokines by multiarmed poly(ethylene glycol)-glycosaminoglycan (starPEG-GAG) hydrogels has recently been shown to support regeneration in a diabetic mouse chronic skin wound model. Herein, a textile-starPEG-GAG composite wound contact layer (WCL) capable of selectively sequestering pro-inflammatory chemokines is reported. Systematic variation of the local and integral charge densities of the starPEG-GAG hydrogel component allows for tailoring its affinity profile for biomolecular signals of the wound milieu. The composite WCL is subsequently tested in a large animal (porcine) model of human wound healing disorders. Dampening excessive inflammatory signals without affecting the levels of pro-regenerative growth factors, the starPEG-GAG hydrogel-based WCL treatment induced healing with increased granulation tissue formation, angiogenesis, and deposition of connective tissue (collagen fibers). Thus, this biomaterials technology expands the scope of a new anti-inflammatory therapy toward clinical use.
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    Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion
    (Weinheim : Wiley-VCH, 2020) Bekeschus, Sander; Clemen, Ramona; Nießner, Felix; Sagwal, Sanjeev Kumar; Freund, Eric; Schmidt, Anke
    Medical technologies from physics are imperative in the diagnosis and therapy of many types of diseases. In 2013, a novel cold physical plasma treatment concept was accredited for clinical therapy. This gas plasma jet technology generates large amounts of different reactive oxygen and nitrogen species (ROS). Using a melanoma model, gas plasma technology is tested as a novel anticancer agent. Plasma technology derived ROS diminish tumor growth in vitro and in vivo. Varying the feed gas mixture modifies the composition of ROS. Conditions rich in atomic oxygen correlate with killing activity and elevate intratumoral immune-infiltrates of CD8+ cytotoxic T-cells and dendritic cells. T-cells from secondary lymphoid organs of these mice stimulated with B16 melanoma cells ex vivo show higher activation levels as well. This correlates with immunogenic cancer cell death and higher calreticulin and heat-shock protein 90 expressions induced by gas plasma treatment in melanoma cells. To test the immunogenicity of gas plasma treated melanoma cells, 50% of mice vaccinated with these cells are protected from tumor growth compared to 1/6 and 5/6 mice negative control (mitomycin C) and positive control (mitoxantrone), respectively. Gas plasma jet technology is concluded to provide immunoprotection against malignant melanoma both in vitro and in vivo.
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    Understanding Surface Modifications Induced via Argon Plasma Treatment through Secondary Electron Hyperspectral Imaging
    (Weinheim : Wiley-VCH, 2021) Farr, Nicholas; Thanarak, Jeerawan; Schäfer, Jan; Quade, Antje; Claeyssens, Frederik; Green, Nicola; Rodenburg, Cornelia
    Understanding the effects that sterilization methods have on the surface of a biomaterial is a prerequisite for clinical deployment. Sterilization causes alterations in a material's surface chemistry and surface structures that can result in significant changes to its cellular response. Here we compare surfaces resulting from the application of the industry standard autoclave sterilisation to that of surfaces resulting from the use of low-pressure Argon glow discharge within a novel gas permeable packaging method in order to explore a potential new biomaterial sterilisation method. Material surfaces are assessed by applying secondary electron hyperspectral imaging (SEHI). SEHI is a novel low-voltage scanning electron microscopy based characterization technique that, in addition to capturing topographical images, also provides nanoscale resolution chemical maps by utilizing the energy distribution of emitted secondary electrons. Here, SEHI maps are exploited to assess the lateral distributions of diverse functional groups that are effected by the sterilization treatments. This information combined with a range of conventional surface analysis techniques and a cellular metabolic activity assay reveals persuasive reasons as to why low-pressure argon glow discharge should be considered for further optimization as a potential terminal sterilization method for PGS-M, a functionalized form of poly(glycerol sebacate) (PGS).
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    Introducing the Open Energy Ontology: Enhancing data interpretation and interfacing in energy systems analysis
    (Amsterdam : Elsevier ScienceDirect, 2021) Booshehri, Meisam; Emele, Lukas; Flügel, Simon; Förster, Hannah; Frey, Johannes; Frey, Ulrich; Glauer, Martin; Hastings, Janna; Hofmann, Christian; Hoyer-Klick, Carsten; Hülk, Ludwig; Kleinau, Anna; Knosala, Kevin; Kotzur, Leander; Kuckertz, Patrick; Mossakowski, Till; Muschner, Christoph; Neuhaus, Fabian; Pehl, Michaja; Robinius, Martin; Sehn, Vera; Stappel, Mirjam
    Heterogeneous data, different definitions and incompatible models are a huge problem in many domains, with no exception for the field of energy systems analysis. Hence, it is hard to re-use results, compare model results or couple models at all. Ontologies provide a precisely defined vocabulary to build a common and shared conceptualisation of the energy domain. Here, we present the Open Energy Ontology (OEO) developed for the domain of energy systems analysis. Using the OEO provides several benefits for the community. First, it enables consistent annotation of large amounts of data from various research projects. One example is the Open Energy Platform (OEP). Adding such annotations makes data semantically searchable, exchangeable, re-usable and interoperable. Second, computational model coupling becomes much easier. The advantages of using an ontology such as the OEO are demonstrated with three use cases: data representation, data annotation and interface homogenisation. We also describe how the ontology can be used for linked open data (LOD).
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    Ion dynamics in Al-Stabilized Li7La3Zr2O12 single crystals – Macroscopic transport and the elementary steps of ion hopping
    (Amsterdam : Elsevier, 2020) Posch, Patrick; Lunghammer, Sarah; Berendts, Stefan; Ganschow, Steffen; Redhammer, Günther J.; Wilkening, Alexandra; Lerch, Martin; Gadermaier, Bernhard; Rettenwander, Daniel; Wilkening, H. Martin R.
    Li7La3Zr2O12 (LLZO) garnet-type ceramics are considered as very promising candidates for solid electrolytes and have been extensively studied in the past few years. Several studies report on an increase in ionic conductivity by doping with ions, such as Al3+ and Ga3+, to stabilize the cubic modification of LLZO. Unfortunately, so far ion dynamics have mainly been studied using powdered samples. Such studies may suffer from chemical heterogeneities concerning Al distribution. Here, we took advantage of Al-stabilized LLZO single crystals to throw light on the elementary steps of ion hopping. We used 7Li nuclear magnetic resonance (NMR) spin-lattice relaxation measurements and conductivity spectroscopy to probe dynamic parameters from both a microscopic and macroscopic point of view. At 293 K the total conductivity turned out to be 0.082 mS cm−1, which is remarkably good for LLZO exhibiting an Al-content of only 0.37 wt%. Most importantly, 7Li NMR spin-lock transients revealed two overlapping diffusion-induced processes. Overall, activation energies from spin-lock NMR excellently agree with that from conductivity measurements; both techniques yield values around 0.36 eV. The corresponding diffusion coefficients deduced from NMR and conductivity measurements almost coincide. The magnetic spin fluctuations sensed by NMR provide an in-depth look at the elementary jump processes, which can barely be revealed by macroscopic impedance spectroscopy providing average values. In particular, we were able to precisely measure the local hopping barrier (0.20 eV) characterizing forward-backward jumps between the sites 24d and 96h. © 2019 The Author(s)
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    Corrigendum to “Dynamics of rural livelihoods and rainfall variability in Northern Ethiopian Highlands” [Clim. Risk Manage. 25 (2019) 100195](S2212096318300378)(10.1016/j.crm.2019.100195)
    (Amsterdam [u.a.] : Elsevier, 2020) Adamseged, Muluken E.; Frija, Aymen; Thiel, Andreas
    The authors regret for not properly acknowledging the contribution of the Consortium Research Program (CRP) on Livestock, led by ILRI with contribution of ICARDA. Funding from the CGIAR Research Program on Livestock (flagship 5 on “Livestock livelihoods and agri-food systems flagship”) is acknowledged for their support of staff time of Dr. Aymen Frija. The authors thank all donors and organizations who globally support the work of the CGIAR Research Program on Livestock through their contributions to the CGIAR system. The authors would like to apologise for any inconvenience caused. © 2020 The Author(s)
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    Understanding adaptive capacity of smallholder African indigenous vegetable farmers to climate change in Kenya
    (Amsterdam [u.a.] : Elsevier, 2020) Chepkoech, Winifred; Mungai, Nancy W.; Stöber, Silke; Lotze-Campen, Hermann
    Understanding the adaptive capacity (AC) of farmers is crucial to planning effective adaptation. Action to promote farmers’ AC is required because climate change (CC) is resulting in unpredictable alterations in weather patterns. Based on the sustainable livelihoods framework (SLF), this study explored how access to natural, physical, financial, social and human capitals enhances the AC. Quantitative data from 269 African indigenous vegetable (AIV) farmers in three selected agro-climatic zones in Kenya were analysed. Four indicators in each capital were selected based on previous studies and judgments collected from an expert online ranking survey (n = 35). The Kruskal-Wallis H test and an independent sample t-test were used to test the independence of AC scores and access to the different resources. The findings showed that the majority of farmers (53%) had a moderate AC, while fewer (32%) and (15%) had low or high AC levels respectively. Disparities in adaptive capacity scores were recorded between respondents in terms of their age, marital status and location. Farmers had high access to social capital but low access to financial, natural and human capitals. Female farmers showed lower capacities in the areas of financial, human and natural resources, while their male counterparts had low access to some human and social capitals. Resilient interventions that target individuals with low adaptive capacities are required. © 2020 The Authors