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End date: 2021 × Start date: 2021 × DDC: 540 × DDC: 660 × Type: Article × WGL Institute: DWI ×

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    Polymeric Membranes With Sufficient Thermo‐Mechanical Stability to Deploy Temperature Enhanced Backwash
    (Weinheim : Wiley-VCH, 2021) Aumeier, Benedikt M.; Vollmer, Fabian; Lenfers, Simon; Yüce, Süleyman; Wessling, Matthias
    The alternative membrane cleaning method Temperature Enhanced Backwash exploits elevated temperatures of typically 125 °C to realize high shear rate. This exceeds usual operating temperatures by far. Therefore, the thermo-mechanical properties of polymeric membranes were investigated. A repeated load cycle testing was suited and sensitive to detect the failure of membrane material and potting. All tested PES membranes showed to be stable during the repeated load cycle testing. The potting adhesive may be decisive, thus, a tensile test at 125 °C is proposed. © 2021 The Authors. Chemie Ingenieur Technik published by Wiley-VCH GmbH
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    A Photoclick-Based High-Throughput Screening for the Directed Evolution of Decarboxylase OleT
    (Weinheim : Wiley-VCH, 2021) Markel, Ulrich; Lanvers, Pia; Sauer, Daniel F.; Wittwer, Malte; Dhoke, Gaurao V.; Davari, Mehdi D.; Schiffels, Johannes; Schwaneberg, Ulrich
    Enzymatic oxidative decarboxylation is an up-and-coming reaction yet lacking efficient screening methods for the directed evolution of decarboxylases. Here, we describe a simple photoclick assay for the detection of decarboxylation products and its application in a proof-of-principle directed evolution study on the decarboxylase OleT. The assay was compatible with two frequently used OleT operation modes (directly using hydrogen peroxide as the enzyme's co-substrate or using a reductase partner) and the screening of saturation mutagenesis libraries identified two enzyme variants shifting the enzyme's substrate preference from long chain fatty acids toward styrene derivatives. Overall, this photoclick assay holds promise to speed-up the directed evolution of OleT and other decarboxylases. © 2020 The Authors. Published by Wiley-VCH GmbH
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    Avoiding the Center-Symmetry Trap: Programmed Assembly of Dipolar Precursors into Porous, Crystalline Molecular Thin Films
    (Weinheim : Wiley-VCH, 2021) Nefedov, Alexei; Haldar, Ritesh; Xu, Zhiyun; Kühner, Hannes; Hofmann, Dennis; Goll, David; Sapotta, Benedikt; Hecht, Stefan; Krstić, Marjan; Rockstuhl, Carsten; Wenzel, Wolfgang; Bräse, Stefan; Tegeder, Petra; Zojer, Egbert; Wöll, Christof
    Liquid-phase, quasi-epitaxial growth is used to stack asymmetric, dipolar organic compounds on inorganic substrates, permitting porous, crystalline molecular materials that lack inversion symmetry. This allows material fabrication with built-in electric fields. A new programmed assembly strategy based on metal-organic frameworks (MOFs) is described that facilitates crystalline, noncentrosymmetric space groups for achiral compounds. Electric fields are integrated into crystalline, porous thin films with an orientation normal to the substrate. Changes in electrostatic potential are detected via core-level shifts of marker atoms on the MOF thin films and agree with theoretical results. The integration of built-in electric fields into organic, crystalline, and porous materials creates possibilities for band structure engineering to control the alignment of electronic levels in organic molecules. Built-in electric fields may also be used to tune the transfer of charges from donors loaded via programmed assembly into MOF pores. Applications include organic electronics, photonics, and nonlinear optics, since the absence of inversion symmetry results in a clear second-harmonic generation signal.