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- ItemGround reaction forces and external hip joint moments predict in vivo hip contact forces during gait(Amsterdam [u.a.] : Elsevier Science, 2022) Alves, Sónia A.; Polzehl, Jörg; Brisson, Nicholas M.; Bender, Alwina; Agres, Alison N.; Damm, Philipp; Duda, Georg N.Younger patients increasingly receive total hip arthroplasty (THA) as therapy for end-stage osteoarthritis. To maintain the long-term success of THA in such patients, avoiding extremely high hip loads, i.e., in vivo hip contact force (HCF), is considered essential. However, in vivo HCFs are difficult to determine and their direct measurement is limited to instrumented joint implants. It remains unclear whether external measurements of ground reaction forces (GRFs), a non-invasive, markerless and clinic-friendly measure can estimate in vivo HCFs. Using data from eight patients with instrumented hip implants, this study determined whether GRF time series data, alone or combined with other scalar variables such as hip joint moments (HJMs) and lean muscle volume (LMV), could predict the resultant HCF (rHCF) impulse using a functional linear modeling approach. Overall, single GRF time series data did not predict in vivo rHCF impulses. However, when GRF time series data were combined with LMV of the gluteus medius or sagittal HJM using a functional linear modeling approach, the in vivo rHCF impulse could be predicted from external measures only. Accordingly, this approach can predict in vivo rHCF impulses, and thus provide patients with useful insight regarding their gait behavior to avoid hip joint overloading.
- ItemVisible-NIR ‘point’ spectroscopy in postharvest fruit and vegetable assessment: The science behind three decades of commercial use(Amsterdam [u.a.] : Elsevier Science, 2020) Walsh, Kerry B.; Blasco, José; Zude-Sasse, Manuela; Sun, XudongThe application of visible (Vis; 400–750 nm) and near infrared red (NIR; 750–2500 nm) region spectroscopy to assess fruit and vegetables is reviewed in context of ‘point’ spectroscopy, as opposed to multi- or hyperspectral imaging. Vis spectroscopy targets colour assessment and pigment analysis, while NIR spectroscopy has been applied to assessment of macro constituents (principally water) in fresh produce in commercial practice, and a wide range of attributes in the scientific literature. This review focusses to key issues relevant to the widespread implementation of Vis-NIR technology in the fruit sector. A background to the concepts and technology involved in the use of Vis-NIR spectroscopy is provided and instrumentation for in-field and in-line applications, which has been available for two and three decades, respectively, is described. A review of scientific effort is made for the period 2015 - February 2020, in terms of the application areas, instrumentation, chemometric methods and validation procedures, and this work is critiqued through comparison to techniques in commercial use, with focus to wavelength region, optical geometry, experimental design, and validation procedures. Recommendations for future research activity in this area are made, e.g., application development with consideration of the distribution of the attribute of interest in the product and the matching of optically sampled and reference method sampled volume; instrumentation comparisons with consideration of repeatability, optimum optical geometry and wavelength range). Recommendations are also made for reporting requirements, viz. description of the application, the reference method, the composition of calibration and test populations, chemometric reporting and benchmarking to a known instrument/method, with the aim of maximising useful conclusions from the extensive work being done around the world.
- ItemDiversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids(Amsterdam [u.a.] : Elsevier Science, 2021) Schauer, B.; Szostak, M.P.; Ehricht, R.; Monecke, S.; Feßler, A.T.; Schwarz, S.; Spergser, J.; Krametter-Frötscher, R.; Loncaric, I.Information about livestock carrying methicillin-resistant coagulase-negative staphylococci and mammaliicocci (MRCoNS/MRM) is scarce. The study was designed to gain knowledge of the prevalence, the phenotypic and genotypic antimicrobial resistance and the genetic diversity of MRCoNS/MRM originating from ruminants and New World camelids. In addition, a multi-locus sequence typing scheme for the characterization of Mammaliicoccus (formerly Staphylococcus) sciuri was developed. The study was conducted from April 2014 to January 2017 at the University Clinic for Ruminants and the Institute of Microbiology at the University of Veterinary Medicine Vienna. Seven hundred twenty-three nasal swabs originating from ruminants and New World camelids with and without clinical signs were examined. After isolation, MRCoNS/MRM were identified by MALDI-TOF, rpoB sequencing and typed by DNA microarray-based analysis and PCR. Antimicrobial susceptibility testing was conducted by agar disk diffusion. From all 723 nasal swabs, 189 MRCoNS/MRM were obtained. Members of the Mammaliicoccus (M.) sciuri group were predominant (M. sciuri (n = 130), followed by M. lentus (n = 43), M. fleurettii (n = 11)). In total, 158 out of 189 isolates showed phenotypically a multi-resistance profile. A seven-loci multi-locus sequence typing scheme for M. sciuri was developed. The scheme includes the analysis of internal segments of the house-keeping genes ack, aroE, ftsZ, glpK, gmk, pta1 and tpiA. In total, 28 different sequence types (STs) were identified among 92 selected M. sciuri isolates. ST1 was the most prevalent ST (n = 35), followed by ST 2 (n = 15), ST3 and ST5 (each n = 5), ST4 (n = 3), ST6, ST7, ST8, ST9, ST10 and ST11 (each n = 2).